Effect of toxoplasmic infection on the biodistribution of a brain radiopharmaceutical.

Purpose: This study evaluated if Toxoplasma gondii infection and the drug-associated infection modifies the brain radiopharmaceutical Ethylene Cystine Diethylester Dihydrochloride (99mTc-ECD) biodistribution in mice.Materials and methods: A total of 18 mice were divided into 3 groups. Control group (C) received distilled water and 99mTc-ECD; Infected group (I) received T. gondii strain and 99mTc-ECD; Infected and Treated group (IT), in addition to infection, received association of Pyrimethamine and Sulfadiazine and 99mTc-ECD. The T. gondii strain used in this study was TgCkRrRN3. Forty minutes after administration of the 99mTc-ECD, all animals were euthanized, and blood and brain samples were isolated. The counting of the radioactivity percentage per gram of tissue (%ATI/g) was calculated, and statistical analysis was performed by t-test, with a level of significance of p < .05.Results: There was a significant increase in %ATI/g between groups C and I on brain (0.35 ± 0.02 and 0.45 ± 0.04; p = .041) and on blood (0.80 ± 0.09 and 1.14 ± 0.31; p = .049). A significant decrease in %ATI/g occurred between groups C and IT on blood (0.80 ± 0.09 and 0.54 ± 0.08; p = .001) and on brain (0.35 ± 0.02 and 0.22 ± 0.04; p = .049).Conclusions: Combined therapy of anti-Toxoplasma drugs in infected mice reduced the uptake of 99mTc-ECD, probably due to its binding to plasma proteins.

Seroprevalence of toxoplasmosis in pregnant women in a city in Rio Grande do Norte state, Brazil

Toxoplasmosis is caused by Toxoplasma gondii, which is particularly relevant in pregnant women, due to the risk of congenital infection. The aim of this study was to study the serological profile of pregnant women and analyze some factors related to infection in this group. A descriptive and retrospective study was performed analyzing the results of 356 pregnant women in the Mixed Health Unit of Jaçanã-RN from 2009 to 2014. Results of serological tests for IgG and IgM anti-T. gondii antibodies were provided by LACEN-RN. The chi-square test (χ2) was calculated being considered statistically significant for p<0.05. The results showed that: 59% of the pregnant women tested presented serology IgG anti-T. gondii reagent (IgG+) and IgM anti-T. gondii non-reactive (IgM-), 0.6% reagent for both (IgG+ and IgM+) and 40.4% susceptible to infection (IgG- and IgM-). 71.3% of the women were tested in the first trimester of pregnancy; the 20-29 years of age range was the most prevalent (62.5%) and the 11-19 years of age range was the most susceptible (47%). The rural women had the highest incidence of positive serology (60.2%) and those from the urban area were the most susceptible (40.7%). There was no statistical association between serology and the age group (p=0.282) and origin (p=0.881) variables. There is need for investment in prenatal care and health education for the population about toxoplasmosis