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1.
Bioresour Technol ; 190: 57-65, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25930941

RESUMO

This study focuses on the valorisation of crude glycerol and sunflower meal (SFM) from conventional biodiesel production plants for the separation of value-added co-products (antioxidant-rich extracts and protein isolate) and for enhancing biodiesel production through microbial oil synthesis. Microbial oil production was evaluated using three oleaginous yeast strains (Rhodosporidium toruloides, Lipomyces starkeyi and Cryptococcus curvatus) cultivated on crude glycerol and nutrient-rich hydrolysates derived from either whole SFM or SFM fractions that remained after separation of value-added co-products. Fed-batch bioreactor cultures with R. toruloides led to the production of 37.4gL(-1) of total dry weight with a microbial oil content of 51.3% (ww(-1)) when a biorefinery concept based on SFM fractionation was employed. The estimated biodiesel properties conformed with the limits set by the EN 14214 and ASTM D 6751 standards. The estimated cold filter plugging point (7.3-8.6°C) of the lipids produced by R. toruloides is closer to that of biodiesel derived from palm oil.


Assuntos
Biocombustíveis/microbiologia , Reatores Biológicos/microbiologia , Glicerol/metabolismo , Helianthus/microbiologia , Leveduras/metabolismo , Biodegradação Ambiental , Biocombustíveis/análise , Integração de Sistemas , Leveduras/classificação
2.
Bioresour Technol ; 185: 116-24, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25768413

RESUMO

The main drawbacks of biodiesel production are high reaction temperatures, stirring and time. These could be alleviated by aiding transesterification with alternative energy sources, i.e. ultrasound (US). In this study, biodiesel was obtained from Camelina sativa oil, aided with an ultrasonic probe (20kHz, 70% duty cycle, 50% amplitude). Design of experiments included the combination of sonication and agitation cycles, w/wo heating (50°C). To gain knowledge about the implications of the proposed methodology, conventional transesterification was optimized, resulting in higher needs on catalyst concentration and reaction time, compared to the proposed reaction. Although FAME content met EN 14103 standard, FAME yields were lower than those provided by US-assisted transesterification. Energy consumption measurements showed that ultrasound assisted transesterification required lower energy, temperature, catalyst and reaction time.


Assuntos
Biocombustíveis/efeitos da radiação , Brassicaceae/química , Ácidos Graxos/síntese química , Extratos Vegetais/química , Óleos de Plantas/química , Sonicação/métodos , Brassicaceae/efeitos da radiação , Esterificação/efeitos da radiação , Ácidos Graxos/efeitos da radiação , Ondas de Choque de Alta Energia , Extratos Vegetais/efeitos da radiação , Óleos de Plantas/efeitos da radiação
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