RESUMO
A method of dosage of X Ag factor by laser immunonephelemetry is proposed. This technique, carried out with a rabbit antiserum in the presence of PEG and EDTA, presents the advantage to be sensitive, reproducible and mostly faster than the other immunological methods, such as Electroimmunodiffusion (EID) and Enzyme Linked Immuno-Sorbent Assay (ELISA). It constitutes a simple tool, applicable in the demonstration of an enzyme dysfunction or liver pathology. Finally, it could bring a supplement of information in monitoring antivitamin K therapy.
Assuntos
Antígenos/análise , Fator X/análise , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Imunoensaio/métodos , Imunodifusão/métodos , Lasers , Nefelometria e Turbidimetria/métodosRESUMO
The authors describe an adaptation on a semi- automatique coagulometer KC 10 of a manual assay of anti-Xa activity of the Heparin-Antithrombin III Complex close to the technique described by Yin and al. ( Hepaclot - Stago ). The results show a good linearity and a good reproducibility, with a better sensitivity than the manuel method. Freezing has no influence. Significant differences were observed between our assay and the manuel anti-IIa assay. These last results neither depend on the administration route nor on the heparin batches.
