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1.
Environ Sci Technol ; 58(4): 1823-1831, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-38235527

RESUMO

Air pollution causes morbidity and excess mortality. In the epithelial lining fluid of the respiratory tract, air pollutants trigger a chemical reaction sequence that causes the formation of noxious hydroxyl radicals that drive oxidative stress. For hitherto unknown reasons, individuals with pre-existing inflammatory disorders are particularly susceptible to air pollution. Through detailed multiphase chemical kinetic analysis, we show that the commonly elevated concentrations of endogenous nitric oxide in diseased individuals can increase the production of hydroxyl radicals via peroxynitrite formation. Our findings offer a molecular rationale of how adverse health effects and oxidative stress caused by air pollutants may be exacerbated by inflammatory disorders.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Humanos , Poluentes Atmosféricos/análise , Óxido Nítrico/análise , Óxido Nítrico/farmacologia , Material Particulado/análise , Cinética , Estresse Oxidativo , Poluição do Ar/análise , Radical Hidroxila/análise , Radical Hidroxila/farmacologia
2.
Environ Sci Technol ; 57(34): 12642-12653, 2023 08 29.
Artigo em Inglês | MEDLINE | ID: mdl-37587684

RESUMO

Exposure to ambient air pollution is a major risk factor for human health. Inhalation of air pollutants can enhance the formation of reactive species in the epithelial lining fluid (ELF) of the respiratory tract and can lead to oxidative stress and oxidative damage. Here, we investigate the chemical modification of proteins by reactive species from air pollution and endogenous biological sources using an extended version of the multiphase chemical kinetic model KM-SUB-ELF 2.0 with a detailed mechanism of protein modification. Fine particulate matter (PM2.5) and nitrogen dioxide (•NO2) act synergistically and increase the formation of nitrotyrosine (Ntyr), a common biomarker of oxidative stress. Ozone (O3) is found to be a burden on the antioxidant defense system but without substantial influence on the Ntyr concentration. In simulations with low levels of air pollution, the Ntyr concentration in the ELF is consistent with the range of literature values for bronchoalveolar lavage fluid from healthy individuals. With high levels of air pollution, however, we obtain strongly elevated Ntyr concentrations. Our model analysis shows how chemical reactions of air pollutants can modify proteins and thus their functionality in the human body, elucidating a molecular pathway that may explain air pollutant effects on human health.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Humanos , Tirosina , Estresse Oxidativo
3.
Environ Sci Pollut Res Int ; 30(6): 16749-16755, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36550248

RESUMO

Redox-active substances in fine particulate matter (PM) contribute to inhalation health risks through their potential to generate reactive oxygen species in epithelial lung lining fluid (ELF). The ELF's air-liquid interface (ALI) can play an important role in the phase transfer and multi-phase reactions of redox-active PM constituents. We investigated the influence of interfacial processes and properties by scrubbing of coated nano-particles with simulated ELF in a nebulizing mist chamber. Weakly water-soluble redox-active organics abundant in ambient fine PM were reproducibly loaded into ELF via ALI mixing. The resulting oxidative potential (OP) of selected quinones and other PAH derivatives were found to exceed the OP resulting from bulk mixing of the same amounts of redox-active substances and ELF. Our results indicate that the OP of PM components depends not only on the PM substance properties but also on the ELF interface properties and uptake mechanisms. OP measurements based on bulk mixing of phases may not represent the effective OP in the human lung.


Assuntos
Poluentes Atmosféricos , Material Particulado , Humanos , Material Particulado/análise , Poluentes Atmosféricos/análise , Espécies Reativas de Oxigênio , Oxirredução , Estresse Oxidativo
4.
Environ Sci Technol ; 55(20): 14069-14079, 2021 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-34609853

RESUMO

Air pollution is a major risk factor for human health. Chemical reactions in the epithelial lining fluid (ELF) of the human respiratory tract result in the formation of reactive oxygen species (ROS), which can lead to oxidative stress and adverse health effects. We use kinetic modeling to quantify the effects of fine particulate matter (PM2.5), ozone (O3), and nitrogen dioxide (NO2) on ROS formation, interconversion, and reactivity, and discuss different chemical metrics for oxidative stress, such as cumulative production of ROS and hydrogen peroxide (H2O2) to hydroxyl radical (OH) conversion. All three air pollutants produce ROS that accumulate in the ELF as H2O2, which serves as reservoir for radical species. At low PM2.5 concentrations (<10 µg m-3), we find that less than 4% of all produced H2O2 is converted into highly reactive OH, while the rest is intercepted by antioxidants and enzymes that serve as ROS buffering agents. At elevated PM2.5 concentrations (>10 µg m-3), however, Fenton chemistry overwhelms the ROS buffering effect and leads to a tipping point in H2O2 fate, causing a strong nonlinear increase in OH production. This shift in ROS chemistry and the enhanced OH production provide a tentative mechanistic explanation for how the inhalation of PM2.5 induces oxidative stress and adverse health effects.


Assuntos
Poluentes Atmosféricos , Humanos , Peróxido de Hidrogênio , Radical Hidroxila , Material Particulado , Espécies Reativas de Oxigênio
5.
RSC Adv ; 9(20): 11077-11081, 2019 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-35520271

RESUMO

Cerium oxide nanoparticles (CeNPs) have been shown to exhibit antioxidant capabilities, but their efficiency in scavenging reactive oxygen species (ROS) and the underlying mechanisms are not yet well understood. In this study, cerium dioxide nanoparticles (CeNPs) and nanorods (CeNRs) were found to exhibit much stronger scavenging activity than ·OH generation in phosphate buffered saline (PBS) and surrogate lung fluid (SLF). The larger surface area and higher defect density of CeNRs may lead to higher ·OH scavenging activity than for CeNPs. These insights are important to understand the redox activity of cerium nanomaterials and provide clues to the role of CeNPs in biological and environmental processes.

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