Surface topography of resorbable porcine collagen membranes, and their effect on early osteogenesis: An in vitro study.

OBJECTIVE: Guided tissue regeneration (GTR) is based on the use of different membranes that function as sealants and barriers in specific clinical situations. Among the several tissue production methods and origins, resorbable porcine-derived membranes are the most commonly used. Because these membranes are so diverse, and have several different clinical applications, doubts linger as to their effect in stimulating osteogenesis. The objective of this study was to make an in vitro evaluation of the viability and differentiation of osteoblastic cells cultured on the surface of the following collagen membranes: Jason® (Botiss Biomaterials), Collprotect® (Botiss Biomaterials), and Bio-Gide® (Geistlich). MATERIAL AND METHODS: Fragments of the 3 resorbable collagen membranes (5 × 5 mm) were used, and pre-osteoblastic SAOS-2 cells (ATCC, USA) were plated on their porous surfaces. Evaluation of the membranes was performed at 3, 5 and 7 days, considering the following parameters: (1) topographic analysis of the different surfaces by scanning electron microscope; (2) cellular viability by MTT, (3) quantification of type I collagen and osteopontin by Elisa. The quantitative analyses were carried out using a significance level of 5%. RESULTS: Collprotect® and Jason® membranes presented a rough surface with an irregular aspect on both sides, while double-layered Bio-Gide® had one layer with a smooth surface and the other with a rough surface along each respective length. The viability assays revealed that the cells cultured the cells grown on Collprotect® showed higher viability than those grown in Bio-Gide® or Jason®, especially after 5 and 7 days. After 3 and 5 days, evaluation of type I collagen showed that the cells plated on the Jason® and Collprotect® surfaces had greater collagen secretion than those plated on BioGide®. After 7 days, an increase in osteopontin levels was observed when the cells were plated on all the experimental membranes, compared with the control group. CONCLUSION: All the tested membranes were suitable for use in GTR clinical procedures. Their indication in specific regenerative cases depends on the mechanical and biological properties of their originating tissues, thus enabling better results and assertive choices by dental professionals.

Evaluation of a Titanium Surface Treated with Hydroxyapatite Nanocrystals on Osteoblastic Cell Behavior: An In Vitro Study.

PURPOSE: In the context of macrostructural and microstructural modifications to the design of dental implants, surface topography changes with different treatments have the purpose of accelerating bone formation. The aim of this study was to evaluate in vitro the influence of aggregated hydroxyapatite nanocrystals to surfaces treated with double acid etching (Nano) on osteoblastic cell behavior compared with a conventional double acid-etched surface (DE). MATERIALS AND METHODS: Commercially pure Grade 4 titanium discs (6 × 2 mm) were selected, and both cell proliferation and viability were assessed at 24, 48, and 72 hours using Trypan blue vital dye and MTT, respectively. The expression of type I collagen and osteopontin on such surfaces was evaluated using ELISA. Immunostaining for fibronectin was also performed. Quantitative data were analyzed statistically using two-way analysis of variance (ANOVA) followed by Bonferroni post-test with a 5% significance level. RESULTS: The results showed that in all evaluated time periods, cells expressed fibronectin on both surfaces. The cells presented greater morphologic spreading on the Nano surface when compared with the conventional DE surface in all assessed times. Increased cell proliferation and viability were detected in the Nano surface (P < .05) when compared with the conventional DE surface, especially after 72 hours. Osteopontin expression was higher after 24 hours in the Nano surface when compared with the conventional DE surface (P < .05). For type I collagen, a higher expression was observed with the Nano surface than with the DE surface, again after 72 hours (P < .05). CONCLUSION: This in vitro study showed that the treated Nano surface tested promoted increased cell proliferation and viability when compared with the control surface. Additionally, increased cell spreading as well as type I collagen and osteopontin secretion were observed, favoring the early events of osseointegration.

Peri-implantite: etiologia e tratamento / Periimplantitis: etiology and treatment

A peri-implantite tem sido descrita como uma alteração patológica dos tecidos ao redor dos implantes osseointegrados, sendo a microbiota e o trauma oclusal considerados seus principais fatores etiológicos. A profundidade de sondagem, o sangramento, a mucosa queratinizada, o fluxo do fluido sulcular e o infiltrado inflamatório servem como parâmetros para avaliar a saúde peri-implantar. O tratamento da peri-implantite inclui debridamento mecânico, instrução de higiene oral, destoxificação da superfície implantar, uso de antimicrobianos e terapias ressectivas e regenerativas. Uma correta intervenção parece ser necessária para a resolução da lesão peri-implantar.

The peri-implantitis has been described as a pathological alteration of tissues around dental implants, and the microbiota and occlusal trauma considered its main etiological factors. The probing depth, bleeding, keratinized tissue, the flow of sulcular fluid and inflammatory infiltrate serve as parameters to evaluate the peri-implant health. Treatment of peri-implant includes mechanical debridement, oral hygiene instruction, surface detoxification deploy, use of antibiotics and resective and regenerative therapies. A correct intervention seems necessary for the resolution of the peri-implant injury.

Implante dentário em alvéolo de extração com regeneração óssea guiada – cinco anos de acompanhamento tomográfico feixe cônico / A dental implant in the fresh extraction socket with guided-bone regeneration – a 5-year cone beam computerized tomographic follow-up

A exigência estética nos dias de hoje está junto, senão à frente, dos processos funcionais em reabilitação oral. A perda dentária precoce de dentes anteriores, quando relacionada a casos de severas perdas ósseas, estão entre os casos mais complexos para se atingir resultados positivos na Implantodontia, seja ela estética ou funcional. No presente caso, o paciente apresentou-se com fratura do elemento incisivo central superior esquerdo, com perda óssea considerável na face vestibular e necessitando de reabilitação com implantes osseointegrados e regeneração óssea guiada (ROG), acompanhado de membrana de colágeno reabsorvível. Após o período de osseointegração, foram realizados procedimentos de moldagem e condicionamento dos tecidos ao redor, através de instalação de provisório e posterior confecção de restauração final metalocerâmica. Após acompanhamento tomográfico do caso durante cinco anos, fica evidente a manutenção tecidual e óssea da região citada acima.

Nowadays, the esthetic demands are well-beyond the functional processes for oral rehabilitation. Premature tooth loss in the anterior zone, when related to severe bone loss, are the most complex cases to generate positive results on implant dentistry. In this case, the patient presented with fracture of the maxillary left central incisor, a considerable buccal bone loss, and in need of a dental implant and guided bone regeneration (GBR) with a resorbable collagen membrane. After the osseointegration period, impression procedures and peri-implant soft tissue conditioning were performed by using a provisional restoration before the defi nitive metalloceramic restoration. The 5-year CBCT follow-up revealed soft and hard tissue stable contours in the aforementioned region.

Influence of periodontal biotype on the presence of interdental papillae.

The absence of interdental papillae can be a negative influence in aesthetics. Periodontal biotype (PB) is one of the factors that can possibly influence this relation and has to be considered in periodontal diagnosis and treatment planning. The objective of this study was to evaluate the influence of the periodontal biotype on the presence and absence of interdental papillae. Forty-seven patients were included in this transversal clinical study. The PB, presence and height of interdental papilla, loss of papillary height, and the distance between the base of the contact point and bone crest (CP-BC) were evaluated. The chi-square test was used to verify the significance level of the PB distribution frequency between groups. The Mann-Whitney test was used to compare the CP-BC measures between the different PB groups, and the correlation test was used to verify the relation between the CP-BC distance and the presence of papillae. The thin PB group presented a significantly higher presence of papillae (71.1%) than did the thick PB group (59.6%, P < 0.05). An inverse and proportional correlation between the CP-BC distance and the presence of papillae was found. The authors concluded that the PB influenced the presence and height of interdental papillae.

Instalação de implante com um estágio cirúrgico visando manutenção de estética gengival / Installation of implant with a one surgical time seeking maintenance of gengival aesthetic

O objetivo deste estudo foi analisar através de revisão de literatura e relato de 02 casos clínicos se, as vantagens que se anunciam nos casos de colocação dos implantes imediatamente após as extrações dentárias tais como, redução do tempo de tratamento, preservação da crista óssea, melhora do resultado estético gengival, melhor definição no posicionamento do implante a ser colocado, são reais e viáveis

Perimplantite: etiologia, diagnóstico e tratamento: uma revisão da literatura / Peri-Implantitis: etiology, diagnostic and treatment: a literature review

Diversos estudos indicam a contaminação bacteriana como sendo o agente etiológico da perimplantite, pelo acúmulo de biofilme dento-bacteriano ao redor dos implantes dentais. O objetivo deste estudo foi realizar uma revisão da literatura corrente bem discutir os mecanismos que contribuem para a instalação e perpetuação da infecção perimplantar

Implantes osseointegráveis com coroas provisórias imediatas / Immediate loading of crows in dental implants: a clinical report

A redução no tempo de instalação tem sido objeto de vários estudos com o objetivo de otimizar o tratamento, melhorar a aceitação por parte do paciente, diminuir custo do tratamento, bem como preservar estruturas peri-implantares. a instalação de coroas provisórias imediatas tem sido relatada com grande sucesso. O bojetivo do presente estudo foi realizar uma revisão bibliográfica acerca do tema, bem como relatar um caso clínico com acompanhamento de dois anos

Incidência e severidade da periodontite, e sua importância para a perda do elemento dentário, em uma amostra de militares de duas unidades do Exército brasileiro / Incidence and severety of periodontitis and its importance for loss teeth in population's profile from two military organizations of the Brazilian Army

O objetivo deste estudo foi analisar uma amostra de militares de duas unidades do Exército Brasileiro, a fim de se obter informaçäo sobre a incidência e severidade da periodontite e também, de se avaliar o perfil da amostra, com suas necessidades de tratamento odontológico. Foram examinados 236 pacientes, registrando-se a presença de dentes cariados, extraídos e obturados. Foram feitas mediçöes de profundidade de bolsa, recessäo gengival e consequentemente, nível de perda de inserçäo