RESUMO
A new apparatus for the study of hind-limb inflammation in the rat is described. It measures with high precision the diameters of the hind paws and tibiotarsal joints at anatomically well defined reference points. Inflammation induced by subplantar carrageenin injection and by inoculation of adjuvant in the tail is accompanied by readily detectable and pronounced increases in diameters. Results obtained with this rapid and simple method are reliable and the method offers practical advantages over volume measurements.
Assuntos
Articulação do Tornozelo/anatomia & histologia , Pé/anatomia & histologia , Membro Posterior/anatomia & histologia , Inflamação/patologia , Animais , Articulação do Tornozelo/patologia , Artrite Reumatoide/patologia , Biometria/métodos , Carragenina , Eletrônica , Pé/patologia , Membro Posterior/patologia , Masculino , RatosRESUMO
The moderate incidence of arthritis in Wistar rats after inoculation of a suspension of Mycobacterium butyricum in oil increased to nearly 100% by two modifications. Inoculation with 4 week- as compared to 1 week-cultured. M. butyricum raised the incidence from 34 to 63%. Inoculation of this more arthritogenic material into the F1-descendants of M. butyricum sensitive parents raised the incidence of 96%. Also the severity of the arthritis in rats selected in that manner was higher than in non-selected rats. Treatment of arthritic rats of either source with phenylbutazone resulted in a comparable dose-related reduction of the diameters of the hind-limbs. Mating of rats with various degrees of arthritic response showed that sensitivity transmission to the F1-descendants was optimal with the most sensitive parents. Although the exact nature of the transmission has not been defined, the uniform and higher sensitivity of thus selected rats is of great value for quantitative pharmacological studies.
Assuntos
Artrite Reumatoide/genética , Modelos Animais de Doenças , Ratos Endogâmicos , Adjuvantes Imunológicos , Animais , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/transmissão , Meios de Cultura , Masculino , Mycobacterium , Fenilbutazona/uso terapêutico , RatosRESUMO
A standardized ultraviolet erythema test in guinea-pigs is described in detail. The erythema reaction to 120 sec of UV irradiation was assessed after 1, 2 and 4 h. A direct quantitative comparison of the anti-erythema effect was made between phenylbutazone, indometacin, tolmetin, ketoprofen and alpha-methyl-4-(2-thienylcarbonyl)benzeneacetic acid (suprofen) in a specially designed experiment. All compounds were given by oral gavage 1 h before irradiation. Among the compounds studied suprofen was the most potent antagonist of ultraviolet-induced erythema in guinea-pigs, i.e. about 45 times tolmetin, 25 times phenylbutazone, 10 times indometacin and 5 times ketoprofen activity.
