Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Nanomaterials (Basel) ; 13(16)2023 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-37630959

RESUMO

Electrochemical and optical platforms are commonly employed in designing biosensors. However, one signal readout can easily lead to inaccuracies due to the effect of nonstandard test procedures, different operators, and experimental environments. We have developed a dual-signal protocol that combined two transducer principles in one aptamer-based biosensor by simultaneously performing electrochemical- and extraordinary optical transmission (EOT)-based plasmonic detection using gold nanopit arrays (AuNpA). Compared with full hole structures, we found that nanopits, that did not fully penetrate the gold film, not only exhibited a better plasmonic bandwidth and refractive index sensitivity both in the finite-difference time-domain simulation and in experiments by shielding the gold/quartz mode but also enlarged the electrochemical active surface area. Therefore, the periodic non-fully penetrating AuNpA were modified with ferrocene-labeled human serum albumin aptamer receptors. The formation of the receptor layer and human serum albumin binding complex induced a conformational change, which resulted in variation in the electron transfer between the electro-active ferrocene units and the AuNpA surface. Simultaneously, the binding event caused a surface plasmon polaritons wavelength shift corresponding to a change in the surface refractive index. Interestingly, although both transducers recorded the same binding process, they led to different limits of detection, dynamic ranges, and sensitivities. The electrochemical transducer showed a dynamic detection range from 1 nM to 600 µM, while the optical transducer covered high concentrations from 100 µM to 600 µM. This study not only provides new insights into the design of plasmonic nanostructures but also potentially opens an exciting avenue for dual-signal disease diagnosis and point-of-care testing applications.

2.
J Mater Chem B ; 11(40): 9639-9657, 2023 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-37610228

RESUMO

The combination of electrophysiology and neuroimaging methods allows the simultaneous measurement of electrical activity signals with calcium dynamics from single neurons to neuronal networks across distinct brain regions in vivo. While traditional electrophysiological techniques are limited by photo-induced artefacts and optical occlusion for neuroimaging, different types of transparent neural implants have been proposed to resolve these issues. However, reproducing proposed solutions is often challenging and it remains unclear which approach offers the best properties for long-term chronic multimodal recordings. We therefore created a streamlined fabrication process to produce, and directly compare, two types of transparent surface micro-electrocorticography (µECoG) implants: nano-mesh gold structures (m-µECoGs) versus a combination of solid gold interconnects and PEDOT:PSS-based electrodes (pp-µECoGs). Both implants allowed simultaneous multimodal recordings but pp-µECoGs offered the best overall electrical, electrochemical, and optical properties with negligible photo-induced artefacts to light wavelengths of interest. Showing functional chronic stability for up to four months, pp-µECoGs also allowed the simultaneous functional mapping of electrical and calcium neural signals upon visual and tactile stimuli during widefield imaging. Moreover, recordings during two-photon imaging showed no visible signal attenuation and enabled the correlation of network dynamics across brain regions to individual neurons located directly below the transparent electrical contacts.


Assuntos
Cálcio , Optogenética , Eletrodos Implantados , Optogenética/métodos , Eletrofisiologia/métodos , Ouro
3.
Nat Commun ; 11(1): 6134, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33262332

RESUMO

Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)-a malaria marker-in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays.


Assuntos
Técnicas Biossensoriais/métodos , Imunoensaio/métodos , L-Lactato Desidrogenase/sangue , Malária Falciparum/sangue , Proteínas de Protozoários/sangue , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/genética , Técnicas Biossensoriais/instrumentação , Ouro/química , Humanos , Imunoensaio/instrumentação , L-Lactato Desidrogenase/genética , Malária Falciparum/diagnóstico , Malária Falciparum/parasitologia , Nanopartículas Metálicas/química , Plasmodium falciparum/enzimologia , Plasmodium falciparum/genética , Plasmodium falciparum/isolamento & purificação , Proteínas de Protozoários/genética
4.
Nano Lett ; 20(7): 5243-5250, 2020 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-32520573

RESUMO

Diarylethene molecules are discussed as possible optical switches, which can reversibly transition between completely conjugated (closed) and nonconjugated (open) forms with different electrical conductance and optical absorbance, by exposure to UV and visible light. However, in general the opening reaction exhibits much lower quantum yield than the closing process, hindering their usage in optoelectronic devices. To enhance the opening process, which is supported by visible light, we employ the plasmonic field enhancement of gold films perforated with nanoholes. We show that gold nanohole arrays reveal strong optical transmission in the visible range (∼60%) and pronounced enhancement of field intensities, resulting in around 50% faster switching kinetics of the molecular species in comparison with quartz substrates. The experimental UV-vis measurements are verified with finite-difference time-domain simulation that confirm the obtained results. Thus, we propose gold nanohole arrays as transparent and conductive plasmonic material that accelerates visible-light-triggered chemical reactions including molecular switching.

5.
ACS Appl Mater Interfaces ; 11(50): 46451-46461, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31752486

RESUMO

Microelectrode arrays are widely used in different fields such as neurobiology or biomedicine to read out electrical signals from cells or biomolecules. One way to improve microelectrode applications is the development of novel electrode materials with enhanced or additional functionality. In this study, we fabricated macroelectrodes and microelectrode arrays containing gold penetrated by nanohole arrays as a conductive layer. We used this holey gold to optically excite surface plasmon polaritons, which lead to a strong increase in transparency, an effect that is further enhanced by the plasmon's interaction with cell culture medium. By varying the nanohole diameter in finite-difference time domain simulations, we demonstrate that the transmission can be increased to above 70% with its peak at a wavelength depending on the holey gold's lattice constant. Further, we demonstrate that the novel transparent microelectrode arrays are as suitable for recording cellular electrical activity as standard devices. Moreover, we prove using spectral measurements and finite-difference time domain simulations that plasmonically induced transmission peaks of holey gold red-shift upon sensing medium or cells in close vicinity (<30 nm) to the substrate. Thus, we establish plasmonic and transparent holey gold as a tunable material suitable for cellular electrical recordings and biosensing applications.


Assuntos
Técnicas Biossensoriais , Microeletrodos , Nanoestruturas/química , Ouro/química , Nanotecnologia , Ressonância de Plasmônio de Superfície
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...