[Phospholipase C negatively regulates tyrosine phosphorylation of EGF receptor in A-431 cells]. / Fosfolipaza C negativno reguliruet fosforilirovanie po tirozinu retseptora EFR v kletkakh A-431.

It is known that EGF induces tyrosine phosphorylation and internalization of the EGF receptor in A-431 cells. U73122, an inhibitor of phospholipase C, induces tyrosine phosphorylation of the EGF receptor and its association with phospholipase C still in nonstimulated cells. In U73122 treated cells EGF exerted no effect on these processes. Receptor-mediated endocytosis was not observed in A-431 cells treated with U73122. The reorganization of actin cytoskeleton was detected in U73122 cells.

[Hyperbaric oxygenation in inhalation poisoning with substances with varying mechanisms of action (styrene, toluene and carbon monoxide]. / Giperbaricheskaia oksigenatsiia pri ingaliatsionnoi intoksikatsii veshchestvami s razlichnym mekhanizmom deistviia (stirol, toluol i oksid ugleroda]

The disintoxicating mechanisms of hyperbaric oxygenation (HBO) on inhalation intoxication with substances from different chemical classes (aromatic hydrocarbons and carbon oxide) were investigated in rats. HBO was demonstrated to have a significant effect on the whole array of the studied parameters (physiological, morphological, and biochemical ones), on lipid peroxidation (LP) in particular. The levels of LP products in the liver, brain, blood became normal and the antioxidative system was stimulated as well. The most striking effect of HBO therapy on inhalation of toxic agents taken in the mean or maximum lethal concentrations was noted in the use of carbon oxide.

[Role of hyperbaric oxygenation in the mechanism of ammonium detoxication in resection of the liver in the presence of chronic hepatitis]. / Rol' giperbaricheskoi oksigenatsii v mekhanizme detoksikatsii ammiaka pri rezektsii pecheni na fone khronicheskogo gepatita.

Hyperbaric oxygenation (HBO) used in 170 white rats with chronic CCL4-hepatitis after resection of the liver was conducive to repair of the reversible (glutamine synthesis) and irreversible (urea synthesis) routes of ammonium binding in hepatocytes which are disordered in chronic hepatitis. Hyperbaric oxygen regulates the effect of resection of the liver on the glutamine- and urea-synthesizing function of the hepatocytes in chronic hepatitis. Therapeutic effect of HBO persists for 4 days of the posthypoxic period under conditions of posthyperoxic hypoxia.

[Role of oxygen in the vital activity of aerobic organisms]. / Poznanie roli kisloroda v zhiznedeiatel'nosti aérobnykh organizmov.

New experimental data that underlie construction of an adaptive-metabolic conception as to curative effect of hyperbaric oxygenation are systematized in the aspect of evolution. Oxygen under high pressure (300 kPa, 60 min) exerts a specific effect on functions of redox and antiredox systems and nonspecific effect determining adaptation mechanisms of functional (regulatory, effector), metabolic (bioenergy, detoxication, biosynthetic) and morphogenetic (cellular and intracellular regeneration) types at different (hyper-, normo- and hypobiotic) levels of vital activity as dependent on the metabolic activity of the functional structures under pathology conditions.

[Effects of hyperbaric oxygenation on activation of immunocompetent cells and lipid peroxidation under immunization by heterologous erythrocytes]. / Vliianie giperbaricheskoi oksigenatsii na aktivatsiiu immunokompetentnykh kletok i perekisnogo okisleniia lipidov pri immunizatsii geterologichnymi éritrotsitami.

On the basis of rats' experiments under conditions of HBO a close relationship was established between the intensity of immune processes and peroxide oxidation of lipids (POL). The study enabled to show a connection between immunoreactivity stimulation in spleen cells and certain levels of POL intensity and anti-oxidation protection as well. There were assessed diverse HBO effects on intact and immune organism and hyperbaric dependence of immunodepression on POL's intensification.

[The vitamin E concentration and lipid peroxidation status of patients with peptic ulcer undergoing laser therapy]. / Kontsentratsiia vitamina E i sostoianie perekisnogo okisleniia lipidov u bol'nykh iazvennoi bolezn'iu pri lazeroterapii.

The authors established the necessity that during laser therapy of patients with ulcer disease this treatment should be associated with the use of cholinolytic agents and antioxidants of the alpha-tocopherol type. As the authors' data indicate in ulcer disease a deficit of vitamin E and an enhancement of lipid peroxidation is present.

[Action of anti-arrhythmia agents in ischemia and hyperoxia]. / Deistvie nekotorykh protivoaritmicheskikh sredstv v usloviiakh ishemii i giperoksii.

During experiments (128 rabbits) it was shown that under ischemia and hyperoxia the pharmacodynamics of lidocaine, anaprilin and ethacizine changed in centrogenic and peripheral arrhythmias induced by aconitine. Hyperoxia significantly potentiated the antiarrhythmic effect of the drugs except lidocaine action in centrogenic arrhythmias.

[Enzyme activity and content of glycerophosphate shuttle metabolites in the cortex and medulla oblongata during hyperbaric oxygenation therapy of acute hemorrhage]. / Aktivnost' fermentov i soderzhanie metabolitov glitserofosfatnogo chelnochnogo tsikla v kore i prodolgovatom mozge pri giperbaricheskoi kislorodnoi terapii ostroi krovopoteri.

The experiments on cats have demonstrated that hyperbaric oxygenation (303.9 kPa, 60 min) in acute blood loss (24.0 +/- 0.8 ml/kg) has an essential effect on glycerophosphate shuttle mechanism: it stimulates mitochondrial glycerol-3-phosphate dehydrogenase in the sensorimotor cortex and medulla oblongata, providing glycerol-3-phosphate dehydrogenation, activates cytoplasm hydrogen delivery to mitochondrial respiratory chain, prevents glycerol-3-phosphate and NADH level increase and cytoplasm glycerol-3-phosphate dehydrogenase inhibition, limiting lactate production.

[Modeling of myocardial lesions of varying degrees of manifestation]. / Modelirovanie porazhenii miokarda razlichnoi stepeni vyrazhennosti.

Five variants of myocardial lesions of different degree were simulated in experiments on 8 dogs, 61 rabbits and 30 rats, namely micronecrosis, diffuse myocarditis, focal dystrophy, small- and large-focal myocardial infarction. The experimental models of heart lesions can be used for studying the pathophysiological mechanisms in cardiac pathology and evaluation of the therapeutic efficacy of new drugs in experimental pharmacology.

[Quantitative evaluation and prognosis of the biological effects of hyperoxia]. / O kolichestvennoi otsenke i prognozirovanii biologicheskikh éffektov giperoksii.

Experiments in 104 white mice subjected to hyperoxia at different regimens of oxygenation, carried out by means of mathematical modelling of three hyperoxic effects (bradypnea, convulsions, lethality) revealed the dependence of biological (physiological, toxic) effects of hyperoxia upon both components of the oxygenation regimen (oxygen pressure, time of exposure). The proposed concept of integral dose allows to determine pressure-exposure correlations corresponding to a given biological phenomenon and to prognosticate the effects of hyperoxia.

[Importance of adrenoreactive vessels in the mechanisms of the vasoconstrictor response to hyperoxia]. / Znachenie adrenoreaktivnosti sosudov v mekhanizmakh vazokonstrik-tornoi reaktsii na giperoksiiu.

Experiments in 116 white rats showed that high--pressure oxygen (3 beta 3.9 kPa--50 min) increased the adrenoreactivity of vessels under stimulation of alpha--adrenoreceptors with mesaton (phenylephrine) and lost this ability after blockade with pyroxan. The vasoconstricting response (the increase of blood pressure and of general peripheral resistance of vessels) seem to stem from interaction of high--pressure oxygen with post--synaptic alpha--adrenoreceptors of vessels.