Impact of disturbance on common scoter carrying capacity based on an energetic model.

Shallow coastal zones are intensely used by humans but simultaneously are biodiversity hotspots, with a crucial role in the life cycle of many marine species. The competition for food or space between humans and protected wildlife intensifies under pressure of an increased need for marine resources for human consumption. For successful management it is important to establish the key components driving such conflicts of interest. Here we focus on the protected common scoter (Melanitta nigra), a sea duck wintering in coastal habitats that are rich in food, but also among the most disturbed marine systems worldwide. Due to the scoters' shyness disturbance impacts the birds' ability to forage and poses a conflict for balancing bird conservation and economics, including a fishery on its main bivalve prey Spisula subtruncata. In this study, we use an energy budget model to quantify the consequences of depth, currents and disturbance on scoter energetics and carrying capacity. Energetics were described using physical parameters and field data on food availability and disturbance. Results reveal non-linear relationships and a threshold value for when a scoter can no longer maintain its energy balance. This is caused by limited foraging time, rather than food availability. From a conservation perspective, this implies that a precautionary principle should be used, because there will be no warning when an area becomes unsuitable. In addition, the model was applied to study the effects of disturbance from different kinds of shipping in a coastal area of the North Sea, north of The Netherlands. Cargo shipping has the largest impact on the carrying capacity, where there is spatial overlap of prey and an intensively used shipping lane. In other prey distribution situations shrimp vessels may cause most disturbance. Spisula-Ensis fisheries did not limit the potential carrying capacity due to the limited catches and number of fishing trips. Scoter protection should be aimed at flexible spatial management and on only those vessel types above a Spisula bed with a large number of trips, and above all should work from a precautionary principle given the critical thresholds for scoter presence.

Microplastic in a macro filter feeder: Humpback whale Megaptera novaeangliae.

Marine filter feeders are exposed to microplastic because of their selection of small particles as food source. Baleen whales feed by filtering small particles from large water volumes. Macroplastic was found in baleen whales before. This study is the first to show the presence of microplastic in intestines of a baleen whale (Megaptera novaeangliae). Contents of its gastrointestinal tract were sieved, dissolved in 10% potassium hydroxide and washed. From the remaining dried material, potential synthetic polymer particles were selected based on density and appearance, and analysed by Fourier transform infrared (FTIR) spectroscopy. Several polymer types (polyethylene, polypropylene, polyvinylchloride, polyethylene terephthalate, nylon) were found, in varying particle shapes: sheets, fragments and threads with a size of 1mm to 17cm. This diversity in polymer types and particle shapes, can be interpreted as a representation of the varying characteristics of marine plastic and the unselective way of ingestion by M. novaeangliae.

The effects of shoreface nourishments on Spisula and scoters in The Netherlands.

The coast of The Netherlands is protected by nourishing sand. Generally, two different techniques are used, beach nourishment and shoreface nourishment. The latter technique supplies sand at a water depth of about 5-8m in the surf zone, and has been used on a regular basis since 1997 with increasing volumes since 2001. Observations on the bivalve mollusc Spisula subtruncata that was abundant before 1997 and a key food species for wintering seaduck show a decline since 2001. This coincided with a decrease in the abundance of the Common Scoter Melanitta nigra, the most numerous wintering seaduck off the Dutch coast. These observations raised concern about shoreface nourishments. This study analyses the timing and locations of shoreface nourishments in combination with S. subtruncata abundance and spatial distribution. Against the expectation, no causal relationship was found between the decline of S. subtruncata and shoreface nourishments. Other causes, such as climate change, fisheries, unsuccessful settlement or predation of spatfall are more likely behind the decline of Spisula along the Dutch coast.

Resonance assignment strategies for the analysis of NMR spectra of proteins.

Determination of the high resolution solution structure of a protein using nuclear magnetic resonance (NMR) spectroscopy requires that resonances observed in the NMR spectra be unequivocally assigned to individual nuclei of the protein. With the advent of modern, two-dimensional NMR techniques arose methodologies for assigning the 1H resonances based on 2D, homonuclear 1H NMR experiments. These include the sequential assignment strategy and the main chain directed strategy. These basic strategies have been extended to include newer 3D homonuclear experiments and 2D and 3D heteronuclear resolved and edited methods. Most recently a novel, conceptually new approach to the problem has been introduced that relies on heteronuclear, multidimensional so-called triple resonance experiments for both backbone and sidechain resonance assignments in proteins. This article reviews the evolution of strategies for the assignment of resonances of proteins.

Structural water in oxidized and reduced horse heart cytochrome c.

The existence of structural water in the interior of both oxidized and reduced horse-heart cytochrome c in solution is demonstrated using nuclear magnetic resonance spectroscopy. Six water molecules have been located in ferrocytochrome c and five in ferricytochrome c, with residence times greater than a few hundred picoseconds. Two water molecules are located in the haem crevice, one of which is found to undergo a large change in position with a change of oxidation state. Both of these observations indicate that buried structural waters in the haem crevice have, by microscopic dielectric effects, significant roles in the setting of the solvent reorganization energy associated with electron transfer.