Convergent and construct validity and test-retest reliability of the Caen Chronotype Questionnaire in six languages.

Chronotype questionnaires provide a simple and time-effective approach to assessing individual differences in circadian variations. Chronotype questionnaires traditionally focused on one dimension of chronotype, namely its orientation along a continuum of morningness and eveningness. The Caen Chronotype Questionnaire (CCQ) was developed to assess an additional dimension of chronotype that captures the extent to which individual functioning varies during the day (amplitude). The aim of this study was to provide a multilanguage validation of the CCQ in six world regions (Arabic, Dutch, German, Italian, Portuguese and Spanish). At Time 1, a total of 2788 participants agreed to take part in the study (Arabic, n = 731; Dutch, n = 538; German, n = 329; Italian, n = 473; Portuguese, n = 361; Spanish, n = 356). Participants completed an assessment of the CCQ together with the Morningness-Eveningness Questionnaire (MEQ; Horne & Ostberg 1976) as well as questions related to factors theoretically related to chronotype (age, shift work, physical activity, sleep parameters and coffee consumption). One month later, participants again completed the CCQ. Results showed that the two-factor structure (morningness-eveningness and amplitude) of the CCQ could be replicated in all six languages. However, measurement invariance could not be assumed regarding the factor loadings across languages, meaning that items loaded more on their factors in some translations than in others. Test-retest reliability of the CCQ ranged from unacceptable (German version) to excellent (Dutch, Portuguese). Convergent validity was established through small-medium effect size correlations between the morningness-eveningness dimension of the CCQ and the MEQ. Taken together, our findings generally support the use of the translated versions of the CCQ. Further validation work on the CCQ is required including convergent validation against physiological markers of sleep, health and well-being.

Interaction between graviception and carotid baroreflex function in humans during parabolic flight-induced microgravity.

The aim of the present study was to assess carotid baroreflex (CBR) function during acute changes in otolithic activity in humans. To address this question, we designed a set of experiments to identify the modulatory effects of microgravity on CBR function at a tilt angle of -2°, which was identified to minimize changes in central blood volume during parabolic flight. During parabolic flight at 0 and 1 g, CBR function curves were modeled from the heart rate (HR) and mean arterial pressure (MAP) responses to rapid pulse trains of neck pressure and neck suction ranging from +40 to -80 Torr; CBR control of HR (carotid-HR) and MAP (carotid-MAP) function curves, respectively. The maximal gain of both carotid-HR and carotid-MAP baroreflex function curves were augmented during microgravity compared with 1 g (carotid-HR, -0.53 to -0.80 beats·min-1·mmHg-1, P < 0.05; carotid-MAP, -0.24 to -0.30 mmHg/mmHg, P < 0.05). These findings suggest that parabolic flight-induced acute change of otolithic activity may modify CBR function and identifies that the vestibular system contributes to blood pressure regulation under fluctuations in gravitational forces. NEW & NOTEWORTHY The effect of acute changes in vestibular activity on arterial baroreflex function remains unclear. In the present study, we assessed carotid baroreflex function without changes in central blood volume during parabolic flight, which causes acute changes in otolithic activity. The sensitivity of both carotid heart rate and carotid mean arterial pressure baroreflex function was augmented in microgravity compared with 1 g, suggesting that the vestibular system contributes to blood pressure regulation in humans on Earth.

Effect of an acute increase in central blood volume on cerebral hemodynamics.

Systemic blood distribution is an important factor involved in regulating cerebral blood flow (CBF). However, the effect of an acute change in central blood volume (CBV) on CBF regulation remains unclear. To address our question, we sought to examine the CBF and systemic hemodynamic responses to microgravity during parabolic flight. Twelve healthy subjects were seated upright and exposed to microgravity during parabolic flight. During the brief periods of microgravity, mean arterial pressure was decreased (-26 ± 1%, P < 0.001), despite an increase in cardiac output (+21 ± 6%, P < 0.001). During microgravity, central arterial pulse pressure and estimated carotid sinus pressure increased rapidly. In addition, this increase in central arterial pulse pressure was associated with an arterial baroreflex-mediated decrease in heart rate (r = -0.888, P < 0.0001) and an increase in total vascular conductance (r = 0.711, P < 0.001). The middle cerebral artery mean blood velocity (MCA Vmean) remained unchanged throughout parabolic flight (P = 0.30). During microgravity the contribution of cardiac output to MCA Vmean was gradually reduced (P < 0.05), and its contribution was negatively correlated with an increase in total vascular conductance (r = -0.683, P < 0.0001). These findings suggest that the acute loading of the arterial and cardiopulmonary baroreceptors by increases in CBV during microgravity results in acute and marked systemic vasodilation. Furthermore, we conclude that this marked systemic vasodilation decreases the contribution of cardiac output to CBF. These findings suggest that the arterial and cardiopulmonary baroreflex-mediated peripheral vasodilation along with dynamic cerebral autoregulation counteracts a cerebral overperfusion, which otherwise would occur during acute increases in CBV.

Diurnal variation in gait characteristics and transition speed.

The aim of this study was to investigate the effect of time-of-day on Preferred Transition Speed (PTS) and spatiotemporal organization of walking and running movements. Twelve active male subjects participated in the study (age: 27.2 ± 4.9 years; height: 177.9 ± 5.4 cm; body mass: 75.9 ± 5.86 kg). First, PTS was determined at 08:00 h and 18:00 h. The mean of the two PTS recorded at the two times-of-day tested was used as a reference (PTSm). Then, subjects were asked to walk and run on a treadmill at three imposed speeds (PTSm, PTSm + 0.3 m.s(-1), and PTSm - 0.3 m.s(-1)) at 08:00 h and 18:00 h. Mean stride length, temporal stride, spatial stride variability, and temporal stride variability were used for gait analysis. The PTS observed at 08:00 h (2.10 ± 0.17 m.s(-1)) tends to be lower (p = 0.077) than that recorded at 18:00 h (2.14 ± 0.19 m.s(-1)). Stride lengths recorded while walking (p = 0.038) and running (p = 0.041) were shorter at 08:00 h than 18:00 h. No time-of-day effect was observed for stride frequency during walking and running trials. When walking, spatial stride variability (p = 0.020) and temporal stride variability (p = 0.028) were lower at 08:00 h than at 18:00 h. When running, no diurnal variation of spatial stride variability or temporal stride variability was detected.

Regional redistribution of blood flow in the external and internal carotid arteries during acute hypotension.

The present study examined to what extent an acute bout of hypotension influences blood flow in the external carotid artery (ECA) and the corresponding implications for blood flow regulation in the internal carotid artery (ICA). Nine healthy male participants were subjected to an abrupt decrease in arterial pressure via the thigh-cuff inflation-deflation technique. Duplex ultrasound was employed to measure beat-to-beat ECA and ICA blood flow. Compared with the baseline normotensive control, acute hypotension resulted in a heterogeneous blood flow response. ICA blood flow initially decreased following cuff release and then returned quickly to baseline levels. In contrast, the reduction in ECA blood flow persisted for 30 s following cuff release. Thus, the contribution of common carotid artery blood flow to the ECA circulation decreased during acute hypotension (-10 ± 4%, P < 0.001). This finding suggests that a preserved reduction in ECA blood flow, as well as dynamic cerebral autoregulation likely prevent a further decrease in intracranial blood flow during acute hypotension. The peripheral vasculature of the ECA may, thus, be considered an important vascular bed for intracranial cerebral blood flow regulation.

Morning anaerobic performance is not altered by vigilance impairment.

The aim of this study was to determine the role played by vigilance on the anaerobic performance recorded during a Wingate test performed at the bathyphase (nadir) of the circadian rhythmicity. Twenty active male participants performed a 60-s Wingate test at 6 a.m. during 3 test sessions in counter-balanced order the day after either (i) a normal reference night, (ii) a total sleep deprivation night, or (iii) a total sleep deprivation night associated with an extended simulated driving task from 9 p.m. to 5 a.m. During this task, the number of inappropriate line crossings (ILCs) was used to control and quantify the effective decrease in the level of vigilance. The main findings show that (i) vigilance of each participant was significantly altered (i.e., a drastic and progressive increase in ILCs is shown during the 7.5 hours of driving) by the sleep deprivation night associated with an extended driving task; (ii) the subjective evaluation of vigilance performed by self-rated scale revealed an increased impairment of the vigilance level between the normal reference night, the total sleep deprivation night and the total sleep deprivation night associated with an extended driving task; and (iii) the morning following this last condition, during the Wingate test, the recorded cycling biomechanical parameters (peak power, mean power and fatigue index values, power decrease, and cycling kinetic and kinematic patterns) were not significantly different from the two other conditions. Consequently, these results show that anaerobic performances recorded during a Wingate test performed at the bathyphase of the circadian rhythmicity are not altered by a drastic impairment in vigilance. These findings seem to indicate that vigilance is probably not a factor that contributes to circadian variations in anaerobic performance.

Validation of a chronotype questionnaire including an amplitude dimension.

The aim of this paper was to validate a French version of the Chronotype Questionnaire (Oginska, 2011, Pers. Individ. Dif. 50:1039-1043), which represents an interesting novelty in the psychometric assessment of chronotype, because it comprises not only an assessment of the morningness-eveningness (ME) dimension, but also a distinctness (DI) dimension (i.e., amplitude), which represents the range of diurnal variation. In study 1, we aimed to confirm the structure of the Chronotype Questionnaire, with two different samples, young adults (n = 338, mean ± SD = 18.70 ± 1.12 yrs, 244 men and 94 women) and old adults (n = 477, mean ± SD = 55.92 ± 11.9 yrs, 168 men and 310 women). The confirmatory factor analysis (CFA) indicated a poor fit in both samples as well as in the whole sample, which could be due to some inconsistencies in the original instrument, above all in the distinctness dimension. We therefore decided to revise the Chronotype Questionnaire, keeping the ME dimension, but refining the DI dimension. In study 2, with a new sample of 197 participants (mean ± SD = 22.71 ± 2.23 yrs, 105 men and 92 women), we examined the factor structure of the revised scale containing 18 items. The resulting questionnaire contained 16 items (i.e., 8 items on each scale), with item factor loadings higher than .45. In study 3, we aimed to confirm the factor structure of the instrument developed in study 2 as well as to examine its convergent validity, with a new sample of 158 participants (mean ± SD = 55.92 ± 11.9 yrs, 97 men and 61 women). Results of the CFA showed that a good fit of the model could be obtained with 16 items in the questionnaire. The new questionnaire derived from the original Chronotype Questionnaire was from now on called the Caen Chronotype Questionnaire (CCQ). Convergence validity was obtained with the Horne and Östberg questionnaire and the ME scale of the CCQ, showing a negative significant relationship (r = -.82). The CCQ showed promising psychometric qualities, and further research should aim to combine it with physiological variables.

Time-of-day effects on fatigue during a sustained anaerobic test in well-trained cyclists.

The aim of this study was to evaluate time-of-day effects on fatigue during a sustained anaerobic cycling exercise. Sixteen healthy male competitive cyclists were asked to perform a 60 s Wingate test against a braking load of 0.087 kg.kg body mass(-1) during two experimental sessions, which were set up either at 06:00 or 18:00 h in counterbalanced order. There was only one session per day with a recovery period of at least 36 h between the two sessions. Each subject was trained to perform the test. The body mass used to determine the braking load was that of the first test session for each subject and remained constant throughout the two test periods. During the test, peak power (PP), mean power during the first 30 s (MP30 s) and the full 60 s of the test (MP60 s), and fatigue (i.e., the decrease in power output values throughout the exercise) were analyzed. Results confirmed the existence of diurnal variation in anaerobic power output. PP, MP30 s, and MP60 s were significantly higher at 18:00 than 06:00 h, with gains equal to 8.2, 7.8, and 7.8%, respectively. Moreover, all the power output values recorded in the evening were higher than those recorded in the morning, indicating that fatigue induced by this exercise is not affected by time-of-day in male competitive cyclists. It is hypothesized that the freedom and complexity of pedalling could allow adaptations in movement patterns, as a function of time-of-day, in order to maintain higher performance in the evening. For practical considerations, the more complex the movements required to perform a sport, the more the time-of-day effect can be taken into account and adapted to by the trained athlete, particularly in cyclic sporting disciplines such as swimming, running, rowing, and kayaking.