[Polymorphism A1166C of AGTR1 Gene and the State of Intrarenal Blood Flow in Patients with Essential Arterial Hypertension 1-2 Degrees].

AIM: to study relationship between genetic disorders and features of intrarenal blood flow in patients with essential arterial hypertension (AH) of 1-2 degree. MATERIALS AND METHODS: We examined 100 patients (60 women, 40 men) aged 35 to 58 years with 1-2­degree essential arterial hypertension (AH) and chronic kidney disease (CKD) stages I-III. Examination included triplex scanning of renal arteries on the ultrasound scanner Vivid-7 Dimension, genotyping of single-nucleotide polymorphism А1166С of the AGTR1 gene by real time polymerase chain reaction (PCR), estimation of glomerular filtration rate (GFR) using CKD-EPI formula. Patients were divided into 2 groups: group 1 included persons with I and II stage CKD (n=65, 25 men and 40 women), group 2 included patients with stages IIIA and IIIB CKD (n=35, 15 men and 20 women). RESULTS: Among patients of group 1 prevailed genotype AA, while among group 2 patients prevailed genotype AC. Speed of blood flow in interlobar renal arteries was higher in the group 1 compared with group 2, while in the group 2 time of acceleration of blood flow was higher than in the group 1. DISCUSSION: The data obtained are indicative of the decrease of systolic, diastolic, and averaged maximal blood flow velocity and the lengthening of acceleration time in patients with higher CKD stage. CONCLUSIONS: The presence in the genotype of patients with 1-2­degree AH of AGTR1 1166С allele may be considered a risk factor of early development of CKD. Lowering of speed characteristics of blood flow and lengthening of the acceleration time in patients with AH can be a criterion of hypertensive nephropathy development.

[The interrelationship of indices of hemocysteine and genetic polymorphisms conditioning disorders of folates metabolism in healthy population].

The study was carried out to investigate relationship of the level of homocysteine and genetic polymorphisms conditioning disorders of metabolism of folates in healthy population and of various age and gender groups. The study covered 168 donors: 98 males and 70 females. Two gender groups were singled out and in each of them age groups: 18-31, 32-45 and 46-60 years old. The analysis of concentration of homocysteine was implemented using immune chemiluminescence analysis. The polymerase chain reaction was applied for analyzing genetic polymorphisms associated with disorders of folate cycle by genes MTHFR (met hylentetrahydrofolatereductase) (polymorphisms MTHFR: 677 C>T and MTHFR: 1298 A>C), MTR (B12-dependent methionine-synthetase) (polymorphism MTR: 2756 A>G) and MTRR (methionine-synthetase-reductase) (polymorphism MTRR: 66 A>G). The level of homocysteine in blood of donors was reliably higher in male groups of 18-31 and 32-45 years old as against female groups comparable by age. The study established higher rate of occurrence of heterozygous genotypes bringing unfavorable types of polymorphisms of MTHFR and MTRR genes both in male and female groups. The study established higher rate of occurrence of unfavorable genotypes in examined patients in comparison with publications' data. The established statistically significant inverse correlation of concentration of homocysteine and genetic polymorphisms associated with disorders of folate cycle in individuals of young and middle age mainly males, permits to draw a conclusion about absence of their direct relationship. Therefore, implementation of genetic liability to increasing of homocysteine can occur under effect of external unfavorable factors.

[The active neuroprotective therapy of acute carotid ischemic stroke of moderate severity]. / Aktivnaia neiroprotektivnaia terapiia ostrogo karotidnogo ishemicheskogo insul'ta srednei stepeni tiazhesti.

Objective. To evaluate the efficacy of neuroprotective therapy in carotid ischemic stroke. Material and methods. An open comparative study of combined neuroprotective therapy in comparison with standard treatment has been carried out in 25 patients with carotid ischemic stroke of moderate severity in the acute period of disease. Patients received cytoflavin, gliatilin and actovegin, along with basic therapy, during 15 days. Patient's state was assessed every 5 days using the NIHSS. The Barthel index was estimated in the 21st day. Results and conclusion. By the end of treatment, the degree of neurological deficit was significantly lower in patients treated with the combination of the neuroprotectors. The better functional rehabilitation to the 21st day and the positive dynamics of inflammation indices and free-radical processes were shown in patients of the main group.

[Assessment of the level of immunoglobulin E in patients with pyo-inflammatory diseases and its influence on the course of the infectious process].

The level of immunoglobulin E (IgE) in patients with different pyo-inflammatory diseases was assessed and it was found to elevate in patients with acute appendicitis in 49% of cases, in patients with chronic relapsing furunculosis (CRF) in 41%, with chronic osteomyelitis--in 66.6%. In 8 out of 16 examined patients with the elevated level of IgE there were antibodies to toxocars in titers 1:800, 1:400. No correlation with the IgE level was found in assessing the level of IL-4 and gamma-IFN in blood serum of patients with CRF. In patients with acute appendicitis, CRF and chronic osteomyelitis the elevated level of IgE can be considered as a marker of unfavorable course of pyo-inflammatory diseases. The elevated level of IgE does not influence the oxygen-dependent mechanisms of bactericidal action of neutrophils.