[Changes in the lipolytic enzymatic activity of the blood in rats maintained on an atherogenic diet]. / Izmenenie aktivonosti lipoliticheskikh fermentov v plazme krovi krys pri soderzhanii ikh na aterogennom ratsione.

The paper discusses changes in the activity of lipolytic enzymes in blood plasma of rats before and after heparin administration in the course of experimental atherosclerosis development. This development was shown to be characterized by an abrupt rise of preheparin plasma level (comparatively to normal) and of monoacylglycerolipase and tributyrinase activity, with this rise being unchanged throughout the experiment. An increase in the postheparin plasma activity of heparin-dependent lipolytic enzymes, lipoprotein lipase, triacylglycerolipase, monoacylglycerolipase, and tributyrinase was seen only in the initial stage of the disease. After 30-40 weeks the activity of the enzymes was below normal. It is concluded that the increased activity of lipoproteid lipase and triacylglycerolipase is related to their activation in the blood channel.

[Lipid metabolism disorders in white rats following depression of the anticlotting system by an atherogenic diet]. / Narusheniia lipidnogo obmena u belykh krys pri depressii protivosvertyvaiushchei sistemy aterogennoi dietoi.

Experiments conducted with adult rats kept on an therogenic diet for 30 and 45 days demonstrated that with depressed function of the anticoagulation system and developing prethrombotic state of the organism there occur changes in the spectrum of the animals blood serum lipoproteids, namely--an increase of the pre-beta-lipoproteids fraction, the appearance of the chylomicrones fraction and a fall-off of the alpha-lipoproteids fraction. The total content of atherogenic lipoproteids (beta- and pre-beta-lipoproteids) rises by as much as 2-4 times. The depression of the anticoagulation function stemming from alimentary lipemia comes as a menace of forthcoming intravascular thrombogenesis.

[Use of potentiometric titration at constant pH for determination of the activity of lipoprotein lipase]. / Primenenie metoda potentsiometricheskogo titrovaniia pri postoiannom pH dlia opredeleniia aktivnosti lipoproteidlipazy

A method is developed for determination of the lipoprotein lipase activity. The method is based on the steady state potentiometric titration at constant pH value of higher fatty acids, liberated during the hydrolysis. An oil emulsion intralipid, activated by human blood serum, was used as a substrate. The sensitivity of the method was equal to 0.004-0.010 micronM of fatty acids per min. The reproducibility of the results was 2-5%. This simple and rapid method enabled to study kinetic of reactions, catalyzed by lipoprotein lipases.