RESUMO
We present the first nanoscale (down to approximately 50 x 50 nm(2)) detector displaying single-photon sensitivity and a nanosecond response. This type of nanodetector can also be operated in multiphoton mode, where the detection threshold can be set at N = 1, 2, 3, or 4 photons, thus allowing the mapping of photon number statistics on the nanoscale. Its operation principle based on that of hot-spot formation in superconducting nanowires allies the temporal resolution and sensitivity of superconducting single-photon detectors with subwavelength resolution and photon number discrimination. Such detectors can be of great interest for the study of nanophotonic devices at low temperature.
RESUMO
In order to identify genomic changes associated with a resistant phenotype acquisition, we used comparative genomic hybridization (CGH) to compare a human ovarian cell line, Igrov1, and four derived subcell lines, resistant to vincristine and presenting a reversion of malignant properties. Multicolor FISH (Multiplex-FISH and Spectral Karyotype) and conventional FISH are also used to elucidate the karyotype of parental cell line. The drug-resistant subcell lines displayed many chromosomal abnormalities suggesting the implication of different pathways leading to a multidrug resistance phenotype. However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)).
Assuntos
Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/genética , Resistencia a Medicamentos Antineoplásicos/genética , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/genética , Adenocarcinoma/patologia , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Aberrações Cromossômicas , Citogenética , Feminino , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Neoplasias Ovarianas/patologia , Fenótipo , Poliploidia , Vincristina/farmacologiaRESUMO
The velocity and humidity dependence of nanoscopic sliding friction has been studied on CrN and diamondlike carbon surfaces with an atomic force microscope. The surface wettability is found to be decisive. Partially hydrophilic surfaces show a logarithmic decrease of friction with increasing velocity, the slope of which varies drastically with humidity, whereas on partially hydrophobic surfaces we confirm the formerly reported logarithmic increase. A model for the thermally activated nucleation of water bridges between tip and sample asperities fully reproduces the experimental data.
