Use of coumarins as complementary medicine with an integrative approach against cervical cancer: background and mechanisms of action.

Cervical cancer is characterized by the cellular transformation caused by Human Papillomavirus (HPV), favoring cell proliferation, migration, invasion, and metastasis. Cervical cancer is conventionally treated with radiation therapy, and chemotherapy focused on the destruction of tumor cells. However, chemoresistance and low selectivity between tumor and non-tumor cells have been reported, causing side effects in patients. Metabolites of natural origin have shown selectivity against tumor cells, suggesting their use for reducing the side effects caused by drugs used in conventional therapy. Among these compounds, several natural coumarins stand out, such as auraptene, scopoletin, osthole, and praeruptorin, of which antiproliferative, anti-migratory, and anti-invasive activity have been reported. Auraptene, scopoletin, osthole, and praeruptorin show a cytotoxic or antiproliferative effect on cervical tumor cells, arresting the cell cycle by inducing the overexpression of negative regulators of the cell cycle, or inducing cell death by increasing the expression of pro-apoptotic proteins and decreasing that of anti-apoptotic proteins. On the other hand, auraptene, scopoletin, and praeruptorin inhibit the capacity for migration, invasion, and metastasis of cervical tumor cells, mainly by inhibiting the expression and activity of matrix metalloproteinase-2 and -9. The PI3K/Akt signal pathway appears to be central to the anti-tumor activity of the coumarins analyzed in this review. In addition, auraptene, osthole, and praeruptorin are useful in sensitizing tumor cells to radiotherapy or chemotherapeutic molecules, such as FOLFOX, cisplatin, or DOX. Coumarins offer an excellent possibility for developing new drugs as complementary medicine with an integrative approach against cervical cancer.

Artificial cooling as an alternative to increase productivity and welfare of steers under heat stress / Resfriamento artificial como alternativa para aumentar o bem-estar de novilhos sob estresse térmico

The effects of a cooling system on productive efficiency and welfare of Holstein steers were evaluated during the summer. Sixty steers in the finishing phase were randomly allotted to one of two treatment-groups. Animals of control group were only provided with shade (non-cooled group) and a group of animals under a cooling system were installed in the shaded area (cooled group), which were operated daily from 09am to 6pm during the 69d of the study. The averages of environmental temperature and relative humidity were 35.4ºC and 35.3 percent, respectively, with a temperature-humidity index average of 81.4 during the study. Individual body weight was recorded every two weeks, while body surface temperature and respiratory frequency were recorded three times per week. Blood samples were biweekly taken from coccygeal vein for determination of T3 and T4. The average daily gain in the cooled group gain (1.46kg/d) was similar (P=0.21) to non-cooled group (1.37kg/d). Body surface temperature (35.9ºC vs 38.7ºC) and respiratory frequency per minute (77 vs 104) were lower (P<0.01) in the cooled group than in non-cooled group, respectively. Triiodotironine levels were similar (P=0.30) in cooled (0.80ng/mL) and non-cooled (0.87ng/mL) groups, while, tiroxine level was lower (P<0.01) in cooled group (44.0ng/mL) compared to non-cooled group (56.6ng/mL). The cooling system did not improve productive parameters but the welfare in the cooled group was increased.

Avaliou-se um sistema de resfriamento para aumentar a eficiência produtiva e o bem-estar de novilhos durante o verão. Utilizaram-se 60 animais em fase de acabamento, distribuídos aleatoriamente em dois grupos: os do grupo-controle permaneceram somente sob sombra (grupo não resfriado) e os do grupo sob sistema de resfriamento, sob área sombreada, diariamente, das 9 às 18h, durante 69 dias de estudo. As médias registradas de temperatura ambiente e umidade relativa foram 35,4ºC e 35,3ºC, respectivamente, com índice de temperatura-umidade de 81,4 durante o período de estudo. O peso individual foi anotado a cada duas semanas, e a temperatura da superfície corporal e a frequência respiratória foram registrados três vezes por semana. Amostras de sangue foram tomadas da veia coccígea duas vezes por semana para determinação de T3 e T4. O ganho médio diário no grupo sob resfriamento (1,46kg/dia) foi similar (P=0,21) ao do grupo-controle (1,37kg/dia). A temperatura da superfície corporal (35,9ºC versus 38,7ºC) e a frequência respiratória/minuto (77 versus 104) foram mais baixas (P<0,01) no grupo sob resfriamento do que no grupo-controle (P<0,01), respectivamente. Níveis de triiodotironina foram similares (P=0,30) em animais com resfriamento (0,80ng/mL) e sem resfriamento (0,87ng/mL), enquanto o nível de tiroxina foi mais baixo (P<0,01) no grupo com resfriamento (44,0ng/mL versus 56,6ng/mL). O sistema de resfriamento não afetou parâmetros produtivos, porém o bem-estar dos animais do grupo com resfriamento foi melhor.

Salmonella serovars isolated from humans in São Paulo State, Brazil, 1996-2003.

Salmonellosis remains an important cause of diarrheal illness in humans in São Paulo State, Brazil. In this study were identified 3554 Salmonella isolates from human infections, during the period 1996-2003. Among 68 different serovars determined, S. Enteritidis was the most frequent one in gastrointestinal and extra-intestinal infections accounting for 67.4% of all isolates. S. Typhimurium and S. enterica subsp. enterica (4,5,12:i:-) were most frequently isolated from children aged < 1-4 year-old, in contrast, people with S. Enteritidis infections were most likely to be 20-50 year-old. In our geographic area the continued laboratorial surveillance of salmonellosis, including serotyping, has showed the trends in Salmonella serovars causing infections in humans throughout the time.

Salmonella serovars isolated from humans in São Paulo State, Brazil, 1996-2003 / Sorovares de Salmonella isolados de humanos no Estado de São Paulo, Brasil, 1996-2003

Salmonellosis remains an important cause of diarrheal illness in humans in São Paulo State, Brazil. In this study were identified 3554 Salmonella isolates from human infections, during the period 1996-2003. Among 68 different serovars determined, S. Enteritidis was the most frequent one in gastrointestinal and extra-intestinal infections accounting for 67.4 percent of all isolates. S. Typhimurium and S. enterica subsp. enterica (4,5,12:i:-) were most frequently isolated from children aged < 1-4 year-old, in contrast, people with S. Enteritidis infections were most likely to be 20-50 year-old. In our geographic area the continued laboratorial surveillance of salmonellosis, including serotyping, has showed the trends in Salmonella serovars causing infections in humans throughout the time.

A salmonelose permanece uma importante causa de doença diarréica em humanos no Estado de São Paulo, Brasil. Nesse estudo foram identificados 3554 isolados de Salmonella de infecções humanas, durante o período 1996-2003. Entre os 68 diferentes sorovares determinados, S. Enteritidis foi predominante em infecções gastrointestinais e extra-intestinais, responsável por 67.4 por cento de todos os isolados. S. Typhimurium e S. enterica subsp. enterica (4,5,12:i:-) foram mais freqüentemente isolados de crianças com idade de < 1 a 4 anos, em contraste, infecções por S. Enteritidis foram predominantes em pessoas entre 20 e 50 anos. A contínua vigilância laboratorial das salmoneloses, incluindo a sorotipagem, tem evidenciado os sorovares de Salmonella causando infecções humanas em nossa área geográfica ao longo do tempo.

Continuous performance tests: a comparison of modalities.

Continuous Performance Tests (CPTs) are popular instruments for the assessment of sustained attention and impulsivity in individuals thought to have attentional deficits. CPTs used in research and clinical practice rely on visual presentations of stimulus material with little consideration given to the effects on CPT performance when stimuli are presented in an auditory mode. This study examined CPT performance in normal adult subjects. The mode of presentation (visual or auditory) and the type of task (vigilance or distractibility) were varied and their effects on performance measured. Data were collected on 82 subjects; results indicated that auditory presentation of stimuli increased the difficulty of both tasks. Results also showed that distractibility tasks were more difficult than vigilance tasks.

Tratamiento quirúrgico de la esferocitosis hereditaria. / Surgical trealment of hereditary spherocytosis.

Denucnaimos 11 casos de esferocitosis hereditarios atendidos en el Hospital Nacional "GUILLERMO ALMENARA IRIGOYEN" de 1978 a 1986. Se hace una revisión bibliográfica sobre los aspectos genéticos, patogénos y terapéuticos de este transtorno hematológico.