A new di-recognition and di-functional nanosurface aptamer molecularly imprinted polymer probe for trace glyphosate with SERS/RRS/Abs trimode technique.

A new di-recognition nitrogen-doped carbon dot nanosurface aptamer molecularly imprinted polymer (CDNAg@MIPApt) nanocatalytic di-functional probe was prepared by microwave irradiation. The probe was utilized nitrogen-doped silver carbon dots (CDNAg) as the matrix, glyphosate (Gly) as the template molecule, α-methyl acrylate as the monomer, ethylene glycol dimethacrylate as the cross-linker, and aptamer as the biorecognition element. It could not only recognize Gly but also exhibits catalytic amplification function. It was found that CDNAg@MIPApt catalyzed the redox reaction of polyethylene glycol 400 (PEG400)-AgNO3 to generate silver nanoparticles (AgNPs). The AgNPs indicator component exhibit the effects of surface-enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs). In the presence of Gly, it binds to the surface imprinted site of CDNAg@MIPApt, to reduce AgNPs generation due to the catalytic activity of CDNAg@MIPApt decreasing. Thus, the SERS/RRS/Abs signal values decreased linearly. The linear ranges of SERS/RRS/Abs assay were 0.1-2.5 nM, 0.25-2.75 nM and 0.5-5 nM respectively. The detection limits were 0.034 nM, 0.071 nM and 0.18 nM Gly.

Aptamer SERS and RRS determination of trace lead ions using nitrogen-doped carbon dot to catalyze the new nano-gold reaction.

Nitrogen-doped carbon dots (CDN) were prepared by microwave hydrothermal method using ammonium citrate (AC) and ethylenediaminetetraacetic acid (EDTA) as precursor. It was characterized by transmission electron microscopy (TEM) and infrared spectroscopy (IR). The CDN was found to catalyze the reduction of HAuCl4 to produce gold nanoparticles (AuNP), among which fructose was an effective reducing agent. Using malachite green (MG) as a molecular probe, the surface enhanced Raman scattering (SERS) intensity at 1617 cm-1 and the resonance Rayleigh scattering (RRS) intensity at 375 nm increased linearly with increasing CDN concentration, respectively. The catalytic activity of CDN is inhibited because the aptamer (Apt) can be adsorbed on the surface of the catalyst CDN. The aptamer (Apt)-Pb2+ reaction and CDN-Apt adsorbing reaction were competitive reaction. When there is Pb2+ that binds more tightly to Apt, Apt is desorbed, and the catalytic ability of CDN is restored. Accordingly, an Apt-mediated nanocatalytic amplification SERS/RRS platform for quantitative detection of lead ions was constructed. For the SERS method, the linear range was 0.5-120 nmol/L with DL of 0.11 nmol/L. For the RRS method, the Pb2+ concentration was linear in the range of 50-400 nmol/L with the RRS intensity, and the DL was 15.32 nmol/L. The analysis platform uses CDN catalyzed nanoreactions to generate AuNP products with SERS activity as a substrate, thus overcoming the shortcomings of Pb2+ without scattering activity, and realizing the possibility of SERS and RRS detection of metal ions. It was used for the determination of Pb2+ in real samples with relative standard deviations were 0.94-2.71 % and recovery was 99.00-103.70 %, respectively. In addition, the mechanism of CDN nanoenzyme heterogeneous catalysis of nano-gold reactions was discussed.

Nanogold sol plasmon discattering assay for trace carbendazim in tea coupled aptamer with Au3+-glyoxal-carbon dot nanocatalytic reaction.

Carbendazim (CBZ) is a broad-spectrum fungicide, which is toxic to mammals. Therefore, it is very necessary to establish a sensitive detection for food safety. An experiment found that CDFe exhibited excellent catalysis for the nano-indicator reaction of HAuCl4-glyoxal to produce gold nanoparticles (AuNPs) and that the generated AuNPs have a very strong surface-enhanced Raman scattering (SERS) effect at 1613 cm-1 in the presence of Victoria blue B molecular probes, and resonance Rayleigh scattering (RRS) signals at 370 nm. The aptamer (Apt) suppressed the catalysis of CDFe to cause the SERS and RRS signals decreasing. With the addition of CBZ, the specific Apt reaction occurred to restore the catalysis of CDFe, and resulting in a linear increase in the signals of RRS and SERS. As a result, this new nanocatalytic amplification indicator reaction was coupled with a specific Apt reaction of carbendazim (CBZ), to construct a new CDFe catalytic amplification-aptamer SERS/RRS discattering assay for ultratrace CBZ, which was used to analyze CBZ in tea samples with satisfactory results. In addition, this biosensoring platform can be also used to assay profenofos.

TbMOF@Au catalytic determination of trace malathion with aptamer SERS/RRS/Abs assay.

Terbium metal-organic framework (TbMOF) was prepared by microwave method with 1,3,5-benzenetricarboxylic acid as ligand. With HAuCl4 as precursor and NaBH4 as reducing agent, TbMOF-loaded gold nanoparticles (AuNPs) catalyst (TbMOF@Au1) was prepared rapidly and characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. It was found that TbMOF@Au1 has a strong catalytic effect on the HAuCl4-Cys nanoreaction, and the produced AuNPs have a strong resonant Rayleigh scattering (RRS) peak and surface plasmon resonance absorption (Abs) peak at 370 nm and 550 nm, respectively. With the addition of the molecular probe Victoria blue 4R (VB4r), AuNPs have a strong surface-enhanced Raman scattering (SERS) effect, the target analyte molecules are trapped in between the nanoparticles and a hot spot effect created in the process resulting in an extremely high SERS signal. A new SERS/RRS/Abs triple-mode analysis method for Malathion (MAL) was established by coupling this new TbMOF@Au1 catalytic indicator reaction with MAL aptamer (Apt) reaction, and its SERS detection limit was 0.21 ng/mL. The SERS quantitative analysis method has been applied to the analysis of fruit samples with the recovery of 92.6-106.6 % and the precision of 2.72-8.16 %.

Liquid Crystal@Nanosilver Catalytic Amplification-Aptamer Trimode Biosensor for Trace Pb2.

Liquid crystals (LCs) are a very important display material. However, the use of LC, especially LC-loaded nanoparticles, as a catalyst to amplify the analytical signal and coupled with specific aptamer (Apt) as a recognition element to construct a highly sensitive and selective three-mode molecular spectral assay is rarely reported. In this article, five LCs, such as cholesteryl benzoate (CB), were studied by molecular spectroscopy to indicate the liquid crystal nanoparticles in the system, and highly catalytic and stable CB loaded-nanosilver (CB@AgNPs) sol was prepared. The slope procedure was used to study the catalysis of the five LCs and CB@AgNPs on the new indicator reaction between AgNO3 and sodium formate (Fo) to produce silver nanoparticles (AgNPs) with a strong surface plasmon resonance absorption (Abs) peak at 450 nm, a resonance Rayleigh scattering (RRS) peak at 370 nm and a surface enhanced Raman scattering (SERS) peak at 1618 cm-1 in the presence of molecular probes. By coupling the new CB@AgNPs catalytic indicator reaction with the Apt reaction, a new CB@AgNPs catalytic amplification-SERS/RRS/Abs trimode biosensoring platform was constructed for detecting inorganic pollutants, such as Pb2+, Cd2+, Hg2+ and As3+.

SERS- and absorbance-based catalytic assay for determination of isocarbophos using aptamer-modified FeMOF nanozyme and in situ generated silver nanoparticles.

A new Fe metal-organic framework-loaded liquid crystal 4-octoxybenzoic acid (FeMOF@OCTB) nanosol was synthesized using 1,3,5-phthalic acid, ferrous sulfate, and OCTB as precursors. The FeMOF@OCTB exhibits good stability and strong catalytic effect for the polyethylene glycol 400-Ag (I) indicator reaction, which was evaluated rapidly by the slope procedure. The generated silver nanoparticles have a strong surface-enhanced Raman scattering (SERS) effect and a surface plasmon resonance absorption (Abs) peak at 420 nm. This new bimodal nanosilver indicator reaction was coupled with the isocarbophos (IPS)-aptamer (Apt) reaction. A FeMOF@OCTB nanocatalytic amplified-SERS/Abs bimodal Apt assay for IPS was established. The SERS assay can detect IPS in the concentration range 0.02-1.2 nM, with a detection limit of 0.010 nM. It has been applied to the determination of IPS in rice samples. The relative standard deviation was 4.4-5.8%, and the recovery was 97.7-104%. An Ag nanosol plasmon SERS/Abs dimode aptamer assay was fabricated for trace isocarbophos, based on highly catalysis MOF@OCTB nanoenzyme.

Aptamer Trimode Biosensor for Trace Glyphosate Based on FeMOF Catalytic Oxidation of Tetramethylbenzidine.

The stable and highly catalytic Fe metal-organic framework (FeMOF) nanosol was prepared and characterized by electron microscopy, and energy and molecular spectral analysis. It was found that FeMOF strongly catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce TMBox, which had a fluorescence (FL) peak at 410 nm. When silver nanoparticles were added, it exhibited strong resonance Rayleigh scattering (RRS) activity and surface-enhanced Raman scattering (SERS) effect. This new FeMOF nanocatalytic trimode indicator reaction was combined with the glyphosate aptamer reaction to establish a new SERS/RRS/FL trimode biosensor for glyphosate. The sensor can be used for the analysis of environmental wastewater, and a new method for detecting glyphosate content in wastewater is proposed. The linear range of the sensor is 0.1-14 nmol/L, the detection limit is 0.05 nmol/L, the recovery is 92.1-97.5%, and the relative standard deviation is 3.6-8.7%.

MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb2+ in milk.

Lead ion (Pb2+) is a toxic heavy metal, which is very harmful to organisms. Therefore, the establishment of a rapid, simple, and sensitive method is of great significance to food safety and human health. It was found that MXeneTi3C2 nanosheet (NS) has a strong catalytic effect on the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) via H2O2 to form the oxidized product (TMBOX); it has a strong fluorescence peak at 415 nm and an absorption (Abs) peak at 295 nm. The aptamer of Pb2+ (Aptpb) can be adsorbed on the surface of an NS to form MXene-Apt conjugates, which reduces its catalytic active sites and inhibits its catalytic activity. When the target Pb2+ is added, it specifically binds with Aptpb to release MXene NSs to enhance the dimode signals. Therefore, a new MXene catalytic fluorescence/absorption dimode aptamer biosenering platform was fabricated for the determination of trace Pb2+ in milk and water samples, with the fluorescence assay linear range (LR) of 5.0 × 10-2-2.0 nmol/L.

A novel liquid crystal resonance Rayleigh scattering spectral probe for determination of trace Cr6.

Hexavalent chromium (Cr6+) has strong oxidizing property and toxicity. It has been identified as a carcinogen with obvious carcinogenic effect by the International Anti-cancer Research Center. Therefore, it has a great significance to establish a simple and sensitive method for Cr6+. In the solution, liquid crystal (LC) trans, trans-4-(3,4-difluorophenyl)-4'-n-pentylbicyclohexyl (DP) exhibits strong resonance Rayleigh scattering (RRS) effect due to formation DP nanoparticles. It was used firstly as nanoprobe, to establish a simple and sensitive RRS energy transfer (RRS-ET) method for the determination of trace Cr6+ in water samples. The Cr6+ reacts with diphenylcarbazide (DCB) to produce purple complex. It is adsorbed on the nanoprobe surface, the purple complex as energy receptor and DP as energy donor to produce RRS-ET phenomenon, to make the RRS signal of 370 nm decreasing. In the range of 3-30 nmol/L Cr6+, with the increase of concentration, the RRS signal decreased linearly at 370 nm, with a detection limit of 0.49 nmol/L. This new RRS-ET method was applied to the determination of Cr6+ in water samples, with recovery of 96.0-104.7% and the relative standard deviation (RSD) of 4.44-9.98%.

Ferrocene-Doped Polystyrene Nanoenzyme and DNAzyme Cocatalytic SERS Quantitative Assay of Ultratrace Pb2.

A new, stable and high-catalytic activity ferrocene-doped polystyrene nanosphere (PNFer) sol was prepared by the hydrogel procedure and characterized by electron microscopy and molecular spectroscopy. Results show that the nanosol exhibits excellent catalysis of the new indicator nanoreaction between AgNO3 and sodium formate to generate nanosilver with strong surface-enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs) trimode molecular spectral signals. This new nanocatalytic amplification trimode indicator reaction was coupled with the G-quadruplex DNAzyme catalytic amplification of Pb2+ aptamer to fabricate a new SERS quantitative/RRS/Abs assay platform for the determination of ultratrace amounts of Pb2+. The Pb2+ content in water samples was analyzed with satisfactory results.

On-signal amplification of silver nanosol RRS/SERS aptamer detection of ultratrace urea by polystyrene nanosphere catalyst.

The catalytic amplification signal of polystyrene nanosphere (PN) is used to conveniently fabricate the resonance Rayleigh scattering (RRS)/surface-enhanced Raman scattering (SERS) dual-mode method to sensitively and selectively detect urea in food. PN has strong catalysis of the slow nanoreaction of citrate-Ag(I) to produce yellow silver nanoparticles (AgNP), which exhibit strong RRS effect and SERS effect with molecular probes. When aptamer (Apt) is present, the Apt is adsorbed on the PN surface, the catalysis is weakened, the AgNP is reduced, and the SERS/RRS signal is weakened. After adding urea to exhibit specific Aptamer reaction, the Apt is desorbed from the PN surface and the catalysis is restored. As urea increase, the desorbed PNs increase to produce more AgNPs indicator to increase SERS/RRS signal. The increase value △I of SERS/RRS is linearly to urea concentration. Therefore, a sensitive and selective SERS/RRS dual-mode method for urea is established based on aptamers-regulated the catalysis of PNs. This method is applied to the detection of urea in milk with satisfactory results. The relative standard deviation is 3.9-6.8% and the recovery rate is 94.5-102%.

Highly catalytic nanoenzyme of covalent organic framework loaded starch- surface-enhanced Raman scattering/absorption bi-mode peptide as biosensor for ultratrace determination of cadmium.

High affinity peptides (PTs) have been used in nanoanalysis, but there are no reports which combine PTs with a liquid crystal (LC) covalent organic framework (COF) supported soluble starch (SS) catalytic amplification system as a biosensor recognition element. In this study, a new, highly sensitive and selective bi-mode molecular biosensor has been developed for the determination of cadmium ion (Cd2+). Specifically, a highly catalytic and stable COF supported SS nanosol catalyst was fabricated such that a nanocatalytic indicator reaction system for HAuCl4-sodium formate was established based on surface-enhanced Raman scattering (SERS). The Au nanoparticles produced exhibited a surface plasmon resonance (SPR) absorption peak at 535 nm and a SERS peak at 1,615 cm-1. Combining the nanocatalytic amplification indicator system with the specific PTs reaction permitted a sensitive and selective SERS/absorption bi-mode platform to be developed for the determination of cadmium in rice. The linear range for SERS determination was 0.025-0.95 nmol/L and the detection limit (DL) was 0.012 nmol/L.

A New Covalent Organic Framework of Dicyandiamide-Benzaldehyde Nanocatalytic Amplification SERS/RRS Aptamer Assay for Ultratrace Oxytetracycline with the Nanogold Indicator Reaction of Polyethylene Glycol 600.

In this paper, dicyandiamide (Dd) and p-benzaldehyde (Bd) were heated at 180 °C for 3 h to prepare a new type of stable covalent organic framework (COF) DdBd nanosol with high catalysis. It was characterized by molecular spectroscopy and electron microscopy. The study found that DdBd had a strong catalytic effect on the new indicator reaction of polyethylene glycol 600 (PEG600)-chloroauric acid to form gold nanoparticles (AuNPs). AuNPs have strong resonance Rayleigh scattering (RRS) activity, and in the presence of Victoria Blue B (VBB) molecular probes, they also have a strong surface-enhanced Raman scattering (SERS) effect. Combined with a highly selective oxytetracycline (OTC) aptamer (Apt) reaction, new dual-mode scattering SERS/RRS methods were developed to quantitatively analyze ultratrace OTC. The linear range of RRS is 3.00 × 10-3 -6.00 × 10-2 nmol/L, the detection limit is 1.1 × 10-3 nmol/L, the linear range of SERS is 3.00 × 10-3-7.00 × 10-2 nmol/L, and the detection limit is 9.0 × 10-4 nmol/L. Using the SERS method to analyze OTC in soil samples, the relative standard deviation is 1.35-4.78%, and the recovery rate is 94.3-104.9%.

Aptamer Turn-On SERS/RRS/Fluorescence Tri-mode Platform for Ultra-trace Urea Determination Using Fe/N-Doped Carbon Dots.

Sensitive and selective methods for the determination of urea in samples such as dairy products are important for quality control and health applications. Using ammonium ferric citrate as a precursor, Fe/N-codoped carbon dots (CDFeN) were prepared by a hydrothermal procedure and characterized in detail. CDFeN strongly catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to turn on an indicator molecular reaction, forming an oxidized tetramethylbenzidine (TMBox) probe with surface-enhanced Raman scattering, resonance Rayleigh scattering, and fluorescence (SERS, RRS, and FL) signals at 1,598 cm-1, 370 nm, and 405 nm, respectively. The urea aptamer (Apt) can turn off the indicator reaction to reduce the tri-signals, and the addition of urea turns on the indicator reaction to linearly enhance the SERS/RRS/FL intensity. Thus, a novel Apt turn-on tri-mode method was developed for the assay determination of ultra-trace urea with high sensitivity, good selectivity, and accuracy. Trace adenosine triphosphate and estradiol can also be determined by the Apt-CDFeN catalytic analytical platform.

Single-atom Fe catalytic amplification-gold nanosol SERS/RRS aptamer as platform for the quantification of trace pollutants.

Bisphenol A (BPA), as a typical endocrine disruptor, poses a serious threat to human health. Therefore, it is urgent to establish a rapid, sensitive, and simple method for the determination of BPA. In this paper, based on the aptamer-mediated single-atom Fe carbon dot catalyst (SAFe) catalyzing the HAuCl4-ethylene glycol (EG) nanoreaction, a new SERS/RRS di-mode detection method for BPA was established. The results show that SAFe exhibits a strong catalytic effect on the HAuCl4-EG nanoreaction, which could generate purple gold nanoparticles (AuNPs) with resonance Rayleigh scattering (RRS) signals and surface-enhanced Raman scattering (SERS) effects. After the addition of BPA aptamer (Apt), it could encapsulate SAFe through intermolecular interaction, thus inhibiting its catalytic action, resulting in the reduction of AuNPs generated and the decrease of RRS and SERS signals of the system. With the addition of BPA, Apt was specifically combined with BPA, and SAFe was re-released to restore the catalytic ability; the generated AuNPs increased. As a result of this RRS and SERS signals of the system recovered, and their increment was linear with the concentration of BPA. Thus, the quantification of 0.1-4.0 nM (RRS) and 0.1-12.0 nM (SERS) BPA was realized, and the detection limits were 0.08 nM and 0.03 nM, respectively. At the same time, we used molecular spectroscopy and electron microscopy to study the SAFe-HAuCl4-ethylene glycol indicator reaction, and proposed a reasonable SAFe catalytic reaction mechanism. Based on Apt-mediated SAFe catalysis gold nanoreaction amplification, a SERS/RRS di-mode analytical platform was established for targets such as BPA.

A Highly Sensitive SERS and RRS Coupled Di-Mode Method for CO Detection Using Nanogolds as Catalysts and Bifunctional Probes.

Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di-mode method was developed for CO, based on gold nano-enzyme catalysis and gold nanoprobes. CO can react with HAuCl4 to generate gold nanoparticles (AuNPs) in pH 5.2 HAc-NaAc buffer. The generated AuNPs exhibited SERS activity at 1620 cm-1 in the presence of Vitoria blue B (VBB) molecular probes, and an RRS peak at 290 nm. Based on the AuNP bifunctional probes, the increased SERS and RRS intensities respond linearly with the concentration of CO in the range of 100-1500 ng/mL and 30-5230 ng/mL, respectively. To improve the sensitivity, the produced AuNPs were used as nano-enzyme catalysts for the new indicator reaction of HAuCl4-ethanol (En) to amplify the signal. The sensitive SERS method was coupled with the accurate RRS method to develop a sensitive and accurate SERS/RRS di-mode method for determination of 3.0-413 ng/mL CO, based on the AuNP-HAuCl4-En nanocatalytic reaction and its product of AuNPs as SERS and RRS bifunctional probes.

Silver nanosol SERS quantitative analysis of ultratrace biotin coupled N-doped carbon dots catalytic amplification with affinity reaction.

Highly catalytic and stable N-doped carbon dots (N-CDs) were prepared rapidly by microwave procedure using glucose as precursor and ammonium sulfite as N-dopant. The reduction of AgNO3 by trisodium citrate (TCA) was slow to form nanosilver (AgNP), and the N-CDs exhibited strong catalysis of the AgNP reaction. The formed AgNPs were used as indicator in the presence of Vitoria blue B (VBB) molecule probe with a SERS peak at 1615 cm-1. With the increase of nancatalyst N-CDs concentration, the AgNP reaction speed up, and the SERS peak of VBB enhanced linearly due to formation of more AgNPs as substrate. In the presence of avidin (Ad), the SERS peak weakened. Upon addition of biotin, the SERS peak enhanced due to turn on the indicator nanoreaction. The enhanced SERS signal had a good linear relationship with the biotin concentration in range of 0.0006-0.021 ng/mL, with a detection limit of 0.3 pg/mL.

A new and facile nanosilver SPR colored method for ultratrace arsenic based on aptamer regulation of Au-doped carbon dot catalytic amplification.

Here, Au-doped carbon dots (CDAu) nanosols with good stability were prepared by hydrothermal reaction method. We found that CDAu can efficiently catalyze the nanoreaction of reducing AgNO3 by glucose, and at 420 nm，the reaction products of yellow spherical silver nanosol exhibit an intense surface plasmon resonance (SPR) absorption peak. The nucleic acid aptamers (Apt) can be adsorbed on the surface of carbon dots, so that their catalytic activity was suppressed, the nanosilvers were reduced, the solution color becomes lighter, and the Abs value was weakened. When As3+ was added, it forms a stable conjugate with the Apt, releases free carbon dots, restored its catalytic activity, and the color and Abs signals enhanced linearly. Based on the Apt regulation and the catalytic amplification effect of CDAu on AgNO3-glucose, a new extremely sensitive SPR spectrophotometric method for the determination of arsenic ion content of 0.025-0.75 µg/L was established, and the detection limit of As3+ is 0.01 µg/L.

A highly sensitive and accurate SERS/RRS dual-spectroscopic immunosensor for clenbuterol based on nitrogen/silver-codoped carbon dots catalytic amplification.

Preparation of multifunctional codoped carbon dots, and their new analytical applications in surface-enhanced Raman scattering (SERS) quantitative analytical method are still challenge. To overcome these problems, the nitrogen/silver-codoped carbon dots (CDN/Ag) with highly catalytic amplification are prepared by microwave method, and characterized by spectrophotometry and electron microscopy. The results show that CDN/Ag can strongly catalyze trisodium citrate-HAuCl4 reaction to generate red nanogold with resonance Rayleigh scattering (RRS) effect and SERS effect using Victoria blue B (VBB) as molecular probes. The CDN/Ag catalytic amplification and specific immunoreaction of clenbuterol (Clen) are coupled with highly sensitive SERS and accurate RRS to fabricate a new dual-spectroscopic strategy with a detection limit of 0.68 pg mL-1 Clen.

A novel N/Au co-doped carbon dot probe for continuous detection of silicate and phosphate by resonance Rayleigh scattering.

Co-doped carbon dots are new multifunctional carbon nanomaterials. Their fast preparation and new analytical applications such as in continuous detection and resonance Rayleigh scattering (RRS) probes are of interest to people. Herein, the N/Au co-doped carbon dots (CDN/Au) were prepared quickly by a microwave synthesis method using fructose as the carbon source and urea and HAuCl4 as dopants, and it exhibited an excellent RRS effect at 555 nm. Based on both silicate (SiO32-) and phosphate (PO43-) reacting with ammonium molybdate to form silicomolybdate heteropoly acid (SiMo) and phosphomolybdate heteropoly acid (PMo), PMo decomposed by the addition of citric acid, and SiMo/PMo combined with CDN/Au to show good RRS analytical properties, and a new strategy was developed to detect SiO32- and PO43- by the CDN/Au probes continuously. With the increase of SiO32- (PO43-), SiMo (PMo) reacted with CDN/Au probes to form more big particles which resulted in the RRS intensity enhancement at 555 nm, and had a good linear relationship with the SiO32- (PO43-) concentration in the range of 1.11 µg L-1-19.98 µg L-1, with detection limits of 0.3 µg L-1 SiO32- and 0.3 µg L-1 PO43-. Accordingly, a new RRS method was established for continuous detection of SiO32- and PO43- using CDN/Au as the probe.