Regulation of Autophagy Signaling Pathways by Ginseng Saponins: A Review.

Ginseng saponins ( ginsenosides), bioactive compounds derived from ginseng, are widely used natural products with potent therapeutic properties in the management of various ailments, particularly tumors, cardiovascular and cerebrovascular diseases, and immune system disorders. Autophagy, a highly regulated and multistep process involving the breakdown of impaired organelles and macromolecules by autophagolysosomes and autophagy-related genes (ATGs), has gained increasing attention as a potential target for ginsenoside-mediated disease treatment. This review aims to provide a comprehensive overview of recent research advances in the understanding of autophagy-related signaling pathways and the role of ginsenoside-mediated autophagy regulation. By delving into the intricate autophagy signaling pathways underpinning the pharmacological properties of ginsenosides, we highlight their therapeutic potential in addressing various conditions. Our findings serve as a comprehensive reference for further investigation into the medicinal properties of ginseng or ginseng-related products.

Generation of a modulated versatile spiral beam with varying intensity distribution along the propagation.

A new type of versatile spiral beam (VSB) is generated based on the competition mechanism between the self-focusing property of ring Airy beam and metalens phase distribution, which exhibits twisted properties and optical bottle structure along the propagation direction. The number of spiral lobes, rotation direction, shape and magnification times on the cross section of the proposed beam can be customized by flexibly tuning diffraction distance, topological charge and constant parameter. Therefore, the VSB can be viewed as tunable three-dimensional (3D) spiral beam, and our scheme has the superiority with more diverse and tunable intensity distribution. The properties of intensity distribution variation depended on the propagation distance and topological charge are demonstrated convincingly by employing the Poynting vector intuitive presentation the energy flow. The VSBs with the aid of above-mentioned properties are beneficial for guiding microparticles along the designed spiral path and capturing multiple microparticles into the closed dark regions. Finally, the modulated spiral beams are implemented as tool for particle manipulation in the three dimensional space to demonstrate the advantages of the modulated spiral beam and we can observe the stable trapping of the particles.

Vortex array generation based on quasi-Talbot effects.

A lens-less method for generating vortex arrays with tunable parameters is proposed based on quasi-Talbot effects. By illuminating a two-dimensional periodic sinusoidal grating with a vortex beam carrying a fourth-order cross-phase, the continuous vortex array structure can be generated in the Fresnel diffraction region. Due to the shaping effect of the fourth-order cross-phase on the vortex beam, by changing the constant parameter of the fourth-order cross-phase, it is possible to shape the generation of optical vortex arrays at different positions. This will somewhat broaden the flexibility of the lens-free optical vortex array in terms of generation position. In addition, the generation of polygonal optical vortex arrays is achieved by higher-order cross-phases of different orders. This technique has potential applications in various fields such as optical tweezers, multi-particle screening, microscopic manipulation, etc.

Multiple-dimensional multiplexed holography based on modulated chiro-optical fields.

Orbital angular momentum (OAM) multiplexing technology has been developed in the optical information encryption fields. Here, the modulated chiro-optical OAM (MC-OAM) holography is proposed to further improve information security capacity, which integrates the OAM multiplexing technology with the chiro phase modulation. The orthogonality of the axicon parameter, chiro coefficient and rotation angle modulating the chiro phase distributions are analyzed, respectively, which demonstrate their potential usages as extra degrees of freedom besides the topological charge (TC). Those three parameters combining TC serve as four optical keys, which provides a four-dimensional spatial multiplexing method for information security. Furthermore, we have demonstrated that TC minimum interval of the fractional MC-OAM reaches 0.01. The experimental and simulation results exhibit the essential properties in selectivity and multiplexing of MC-OAM holography. This method can significantly increase the holographic information capacity and safety and inspire widespread applications, such as display, information security and communication.

ContransGAN: Convolutional Neural Network Coupling Global Swin-Transformer Network for High-Resolution Quantitative Phase Imaging with Unpaired Data.

Optical quantitative phase imaging (QPI) is a frequently used technique to recover biological cells with high contrast in biology and life science for cell detection and analysis. However, the quantitative phase information is difficult to directly obtain with traditional optical microscopy. In addition, there are trade-offs between the parameters of traditional optical microscopes. Generally, a higher resolution results in a smaller field of view (FOV) and narrower depth of field (DOF). To overcome these drawbacks, we report a novel semi-supervised deep learning-based hybrid network framework, termed ContransGAN, which can be used in traditional optical microscopes with different magnifications to obtain high-quality quantitative phase images. This network framework uses a combination of convolutional operation and multiheaded self-attention mechanism to improve feature extraction, and only needs a few unpaired microscopic images to train. The ContransGAN retains the ability of the convolutional neural network (CNN) to extract local features and borrows the ability of the Swin-Transformer network to extract global features. The trained network can output the quantitative phase images, which are similar to those restored by the transport of intensity equation (TIE) under high-power microscopes, according to the amplitude images obtained by low-power microscopes. Biological and abiotic specimens were tested. The experiments show that the proposed deep learning algorithm is suitable for microscopic images with different resolutions and FOVs. Accurate and quick reconstruction of the corresponding high-resolution (HR) phase images from low-resolution (LR) bright-field microscopic intensity images was realized, which were obtained under traditional optical microscopes with different magnifications.

Comprehensive analysis of lncRNA expression profiles in rats with cerebral ischemia-reperfusion injury after treatment with 20(R)-ginsenoside Rg3.

This study was aimed at investigating the differentially expressions of long noncoding RNAs (lncRNAs) and mRNAs in the brains of a middle cerebral artery occlusion/reperfusion (MCAO/R) group and a MCAO/R + 20(R)-Rg3 group. Biological enrichment analysis was performed, and a lncRNA-mRNA coexpression network was constructed, to reveal the targets and pathways of 20(R)-Rg3 involved in the regulation of cerebral ischemia-reperfusion injury (CIRI). The RNA-seq high-throughput sequencing method was employed to detect differentially-expressed genes between the groups, which were verified by RT-PCR. Functional enrichment analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were performed to explore the biological functions and relevant pathways. The coexpression network of the screened lncRNAs and mRNAs was built by using Cytoscape software. The results identified 77 upregulated lncRNAs, 162 downregulated lncRNAs, 66 upregulated mRNAs and 472 downregulated mRNAs in the MCAO/R + 20(R)-Rg3 group, compared with those in the MCAO/R group. GO enrichment analysis showed that the GO terms were mainly enriched in stimulation response, cellular response, and stress response. KEGG pathways were mainly related to the tumor necrosis factor (TNF), NF-κB, cytokine, and other receptor signaling pathways. In addition, the coexpression analysis between lncRNA and mRNA identified 314 nodes and 515 connections between 6 lncRNAs and 308 mRNAs, of which 511 were positive and 4 were negative. Among them, ENSRNOG-00000059555 was strongly correlated with AABR07001160.1. This study revealed multiple lncRNAs were involved in the neuroprotection of 20(R)-Rg3 against CIRI and thereby provided new insights into the use of 20(R)-Rg3 as a novel neuro protectant in ischemic stroke management.

Auto-focusing and quantitative phase imaging using deep learning for the incoherent illumination microscopy system.

It is well known that the quantitative phase information which is vital in the biomedical study is hard to be directly obtained with bright-field microscopy under incoherent illumination. In addition, it is impossible to maintain the living sample in focus over long-term observation. Therefore, both the autofocusing and quantitative phase imaging techniques have to be solved in microscopy simultaneously. Here, we propose a lightweight deep learning-based framework, which is constructed by residual structure and is constrained by a novel loss function model, to realize both autofocusing and quantitative phase imaging. It outputs the corresponding in-focus amplitude and phase information at high speed (10fps) from a single-shot out-of-focus bright-field image. The training data were captured with a designed system under a hybrid incoherent and coherent illumination system. The experimental results verify that the focused and quantitative phase images of non-biological samples and biological samples can be reconstructed by using the framework. It provides a versatile quantitative technique for continuous monitoring of living cells in long-term and label-free imaging by using a traditional incoherent illumination microscopy system.