Self-driving dynamic plasmonic colors based on needle steering for simultaneous control of transition direction and time on metallic nanogroove metasurfaces.

Dynamically tunable plasmonic colors hold great promise for a wide range of applications including color displays, colorimetric sensing, and information encryption. However, dynamic control speed of plasmonic colors is still slow to date. Herein, we propose to use a needle to direct the flow of water and gas pressure to drive water, realizing a simultaneous direction-controllable and fast plasmonic color transition. The highly reflected background light of the metallic nanogroove metasurface is suppressed to generate high-purity plasmonic colors through the cross-polarized input and output configuration. When the environment is changed from air to water, a giant color change from cyan to red (a wavelength shift of 156 nm) is experimentally observed. More importantly, by utilizing a needle to steer the flow of water, direction-controllable and fast plasmonic color transition is achieved by controlling gas pressure to drive water. Compared with current state-of-the-art plasmonic color scanning technology, the color transition time via water driven by gas pressure decreases by three orders of magnitude for the same scanning length. The multi-degrees of freedom dynamic structural colors could have potential applications in dynamic displays, anti-counterfeiting, and information security.

Changing antimicrobial susceptibility and molecular characterisation of Neisseria gonorrhoeae isolates in Guangdong, China: in a background of rapidly rising epidemic.

The prevalence of Neisseria gonorrhoeae infections has increased rapidly since 2015 in China. Antimicrobial resistance and molecular mobilisation in N. gonorrhoeae are two important factors driving this increasing prevalence. This study explored changes in antimicrobial susceptibility and molecular characteristics of N. gonorrhoeae collected in Guangdong, China (2013-2017). A total of 704 isolates were collected in two cities in Guangdong. MICs of major antimicrobials were determined. Penicillinase-producing N. gonorrhoeae (PPNG) and tetracycline-resistant N. gonorrhoeae (TRNG) were characterised, and N. gonorrhoeae multiantigen sequence typing (NG-MAST) was performed. High resistance to penicillin (68.2%), tetracycline (85.7%) and ciprofloxacin (98.2%) was observed. Spectinomycin, ceftriaxone and azithromycin appeared effective, with susceptibilities of 100%, 96.4% and 90.7%, respectively. Resistance to penicillin decreased significantly from 78.4% to 73.6% and to azithromycin from 11.9% to 3.7%. Total prevalence of PPNG, TRNG and PPNG/TRNG was 25.4%, 33.1% and 13.4%, respectively. Rates of PPNG decreased significantly from 37.3% to 23.9%, TRNG from 50.0% to 31.3%, and PPNG/TRNG from 23.5% to 11.7%. However, the ratio of African-type PPNG increased significantly (18.4% to 64.1%) compared with decreasing Asian-type PPNG (81.6% to 33.3%), and the ratio of American-type TRNG increased significantly (0% to 13.7%) compared with decreasing Dutch-type TRNG (100% to 86.3%). A total of 271 sequence types (STs) were identified by NG-MAST from 380 isolates collected in 2013, 2014 and 2017, with 145 novel STs. African-type PPNG is increasing and replacing Asian-type, and novel STs have emerged. Gonococcal isolates with new genotypes might contribute to the rising gonorrhoea epidemic in this area.

Tailoring Fano lineshapes using plasmonic nanobars for highly sensitive sensing and directional emission.

Plasmonic oligomers are one class of the most promising nanoclusters for generating Fano resonances. This study reveals that a nanobar-based heptamer concurrently sustains triple polarization-dependent Fano resonances, in sharp contrast to traditional nanodisk or nanosphere-based counterparts. Benefiting from the enhanced near field and reduced spectral linewidth, the gold heptamer exhibits a high refractive index sensitivity (940 nm per RIU) together with a figure of merit (FoM) value as large as 20.9, which outperforms that of most other gold oligomers. On the other hand, it is found that the spectral positions of hybridized eigenmodes depend strongly on the spatial configurations of the constituent nanobars. As a proof of concept, we design a simple heterodimer comprising a nanocross and a nanobar, where plasmonic modes with opposite radiative decay characteristics are excellently overlapped both spectrally and spatially by elaborate tailoring. Double strong Fano resonances appear on opposite sides of the spectrum as expected. More interestingly, the radiation main lobes all point to one direction at these two Fano resonances due to the spatial charge distributions and mode interferences with the maximal directivity ratio (DR) as high as 22.4, in a similar manner to the radio frequency (RF) Yagi-Uda antenna. Furthermore, the emission directions can also be easily switched by adjusting the orientations of the individual nanobar in the heterodimer. Our study demonstrates that the nanobar-based oligomers with tailored Fano lineshapes could serve as versatile and delicate platforms for the label-free biochemical sensing and directional transmission of optical information at the nanometre scale.

Optical magnetism and optical activity in nonchiral planar plasmonic metamaterials.

We investigate optical magnetism and optical activity in a simple planar metamolecule composed of double U-shaped metal split ring resonators (SRRs) twisted by 90° with respect to one another. Compared to a single SRR, the resonant energy levels are split and strong magnetic response can be observed due to inductive and conductive coupling. More interestingly, the nonchiral structures exhibit strong optical gyrotropy (1100°/λ) under oblique incidence, benefiting from the strong electromagnetic coupling. A chiral molecule model is proposed to shed light on the physical origin of optical activity. These artificial chiral metamaterials could be utilized to control the polarization of light and promise applications in enantiomer sensing-based medicine, biology, and drug development.

Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array.

OBJECT: Tuberculosis (TB) continues to be one of the most serious infectious diseases in the world, however, no effective biomarkers can be used for rapid screening of latent tuberculosis infection (LTBI) and active TB. In this study, serum cytokines were screened and tested as potential biomarker for TB diagnosis. METHOD: Cytokine array was used to track the cytokine profile and its dynamic change after TB infection. The different expressions of cytokines were confirmed by ELISA assay. ROC curve analyses were used to evaluate the efficacy of a cytokine or cytokine combination for diagnosis. RESULTS: Eotaxin-2, ICAM-1, MCSF, IL-12p70, and IL-11 were significantly higher in the LTBI individuals. I-309, MIG, Eotaxin-2, IL-8, ICAM-1, IL-6sR, and Eotaxin were significantly higher in active TB patients. ROC curve analyses gave AUCs of 0.843, 0.898, and 0.888 for I-309, MIG, and IL-8, respectively, and 0.894 for the combination panel in active TB diagnosis. IFN-γ/IL-4 and IL-2/TNF-α ratios exhibit dynamic changes in the healthy control and LTBI to different stages of active TB. CONCLUSIONS: Serum cytokines, including I-309 and MIG, IL-8, Extoxin-2, ICAM-1 and combinations of cytokines, including IFN-γ/IL-4 and IL-2/TNF-α, can be used as serum biomarkers for LTBI and active TB screening, thus indicating prospective clinical applications.

[The dynamic changes of tuberculosis related cytokines during disease progression].

OBJECTIVE: To study the dynamic changes of tuberculosis related cytokines among patients during the different courses of treatment, and to analyze their influences on the development and prognoses of tuberculosis. METHODS: All patients with active tuberculosis were enrolled from Guangzhou, Shenzhen and Foshan TB control institutes. There were a total of 68 cases, 36 males and 32 females, aged 19 to 50 years [ average (30±9) years]. All the TB patients received standard chemotherapy regimen of anti-tuberculosis, and were divided into 2 groups: one completed treatment group (cured or clinically cured 38 cases) and 1 uncompleted treatment group (treatment failure or need to extend treatment, 30 cases). Peripheral blood serum at 0, 2, 6 month during the treatment from 68 tuberculosis patients were collected, and the concentration of IFN-γ,IL-4,IL-17,TGF-ß,TNF-α and IL-10 were detected by ELISA tests. RESULTS: The concentration of IFN-γ, TGF-ß and IL-4 in all enrolled patients showed significant decrease (from 23.2 ng/L to 22.3 ng/L, from 169.1 ng/L to 123.2 ng/L; 65.0 ng/L to 31.9 ng/L) (t=2.67, 2.35 and 3.41, P<0.05) along with the extension of treatment. IL-10 increased significantly (12.9 ng/L) in the uncompleted treatment group but declined significantly (5.38 ng/L) (P<0.05) in the completed treatment group at the end of 6 month. Meanwhile, IL-4 decreased significantly (P<0.05) in the completed treatment group but no significant changes were observed in the uncompleted treatment group. Th1/Th2 (IFN-γ/IL-4) raised gradually in the completed treatment group (0 month <2 month <6 month, t=6.32, 6.03 and 5.85, P<0.05), while it was only at 6 month in the uncompleted treatment group (0 month <6 month, t=3.7, P<0.05). And the ratio of Th1/Th2 in the completed treatment group was significantly higher than that in the uncompleted group treatment (P<0.05). CONCLUSION: It suggests that the changes of Th1 cytokines (IFN-γ, TGF-ß) and the Th1/Th2 balance play an important role in the pathogenesis, development and prognosis of TB. The suppression of IFN-γ, TGF-ß or Th1/Th2 balance may be an important factor influencing the prognosis of TB.

Cytokine and Antibody Based Diagnostic Algorithms for Sputum Culture-Positive Pulmonary Tuberculosis.

BACKGROUND: Tuberculosis (TB) is one of the most serious infectious diseases globally and has high mortality rates. A variety of diagnostic tests are available, yet none are wholly reliable. Serum cytokines, although significantly and frequently induced by different diseases and thus good biomarkers for disease diagnosis and prognosis, are not sufficiently disease-specific. TB-specific antibody detection, on the other hand, has been reported to be highly specific but not sufficiently sensitive. In this study, our aim was to improve the sensitivity and specificity of TB diagnosis by combining detection of TB-related cytokines and TB-specific antibodies in peripheral blood samples. METHODS: TB-related serum cytokines were screened using a human cytokine array. TB-related cytokines and TB-specific antibodies were detected in parallel with microarray technology. The diagnostic performance of the new protocol for active TB was systematically compared with other traditional methods. RESULTS: Here, we show that cytokines I-309, IL-8 and MIG are capable of distinguishing patients with active TB from healthy controls, patients with latent TB infection, and those with a range of other pulmonary diseases, and that these cytokines, and their presence alongside antibodies for TB-specific antigens Ag14-16kDa, Ag32kDa, Ag38kDa and Ag85B, are specific markers for active TB. The diagnostic protocol for active TB developed here, which combines the detection of three TB-related cytokines and TB-specific antibodies, is highly sensitive (91.03%), specific (90.77%) and accurate (90.87%). CONCLUSIONS: Our results show that combining detection of TB-related cytokines and TB-specific antibodies significantly enhances diagnostic accuracy for active TB, providing greater accuracy than conventional diagnostic methods such as interferon gamma release assays (IGRAs), TB antibody Colloidal Gold Assays and microbiological culture, and suggest that this diagnostic protocol has potential for clinical application.

Double Fano resonances in plasmonic nanocross molecules and magnetic plasmon propagation.

Double Fano resonances in optical frequency are investigated in an artificial plasmonic molecule consisting of seven identical nanocrosses. These two Fano resonances are found to originate from different physical mechanisms. One is caused by the excitation of the inherent quadrupole dark mode supported by a single nanocross, and the other is attributed to the magnetic plasmon mode due to the generation of antiphase ring currents in adjacent fused tetramers. The two Fano resonances can either be tuned simultaneously or independently within a wide spectral range by adjusting the geometrical parameters of the nanocrosses. The excitation of the magnetic plasmon in a chain made of coupled nanoparticles allows for subwavelength guiding of optical energy with low radiative losses. The field decay length is as long as 2.608 µm, which is comparable to that of the magnetic plasmon waveguides and far surpasses the value achieved in electric plasmon counterparts. Because of the special shape of the nanocross, a Mach-Zehnder interferometer can be built to steer optical beams. These results show that the proposed plasmonic nanostructures have potential applications in biochemical sensing, narrow line-shape engineering and on-chip optical signal propagation in nanoscale integrated optics.

Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library.

Accumulating evidence has suggested that fibroblast growth factor 3 (FGF3) is expressed in breast cancer and correlates with the stage and grade of the disease. In the present study, a specific FGF3­binding peptide (VLWLKNR, termed FP16) was isolated from a phage display heptapeptide library with FGF3. The peptide FP16 contained four identical (WLKN) amino acids and demonstrated high homology to the peptides of the 188­194 (TMRWLKN) site of the high­affinity FGF3 receptor fibroblast growth factor receptor 2. Functional analyses indicated that FP16 mediated significant inhibition of FGF3­induced cell proliferation, arrested the cell cycle at the G0/G1 phase by increasing proliferation­associated protein 2G4, suppressing cyclin D1 and proliferating cell nuclear antigen, and inhibited the FGF3­induced activation of extracellular signal­regulated kinase 1/2 and Akt kinase. Taken together, these results demonstrated that the peptide FP16, acting as an FGF3 antagonist, is a promising therapeutic agent for the treatment of breast cancer.