RESUMO
Toxabramis houdemeri belongs to the genus Toxabramis in the subfamily of Cultrinae (Cyprinidae). We first determine the complete mitochondrial genome of T. houdemeri in this study. It is 16 618 bp in length, with the base composition on the heavy strand: 30.79% A, 16.61% G, 27.29% C, and 25.31% T. It has the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a D-loop region. Phylogenetic analysis showed that T. houdemeri was clustered into one branch of the subfamily of Cultrinae, and closely related to Hemiculter leucisculus. The present study will contribute to genetic resources conservation of T. houdemeri and studying its population genetic structure and phylogenetic relationships.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Filogenia , Animais , Proteínas de Peixes/genética , Genes Mitocondriais , Genes de RNAr , Mitocôndrias/genética , Proteínas Mitocondriais/genética , RNA de Transferência/genéticaRESUMO
Onychostoma barbatum is an endemic species to China. In the present study, the complete mitochondrial genome of O. barbatum in Youjiang River was cloned and analyzed. The complete mitochondrial genome is 16 589 bp in length, with the base composition: A - 31.5%, T - 24.5%, C - 28.1%, and G - 15.9%. It had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a D-loop region. The diversity of sequences and the phylogenetic tree approve that it has high similarity with that in Guijiang River. The mitochondrial genome would contribute to protection of the germplasm resources of O. barbatum and studying its population structure and dynamics in the future.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , China , Cyprinidae/classificação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Ordem dos Genes , Fases de Leitura Aberta/genética , Filogenia , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Rios , Análise de Sequência de DNARESUMO
The threadfin sea catfish (Arius arius) belongs to the genus Arius in Ariidae. In this paper, we initially determined the complete mitochondrial genome of Arius arius. The mitochondrial genome is 16, 711 bp in length, with the base composition on the heavy strand: A - 29.67%, T - 25.38%, C - 29.70% and G - 15.25%. It has the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region. Phylogenetic analysis showed that A. arius was clustered into the order of Siluriformes, and closely related to species in the family of Siluridae. The present study would contribute to genetic resources conservation and systematics study of A. arius.
RESUMO
Distribution and ultrastructural changes in the mitochondrion-rich cells in gills of artificial selected Trachinotus ovatus under different salinities (5, 20, and 30) were examined by light and transmission electron micrograph. Results indicated that the mitochondrion-rich cells were mainly present on the base of the gill filaments and branchial leaflets, and the volume and quantity of mitochondrion-rich cells increased with salinity. All three salinity groups had apical crypts, which were constituted by the mitochondrion-rich cells, pavement cells and accessory cells. Mitochondrion-rich cells in the salinity 5 group had large apical membranes with developed microridges and shallow apical crypts. Apical crypts in the salinity 20 and 30 groups had small apical membranes and undeveloped microridges, and were embolic obviously. Cytoplasm of mitochondrion-rich cells in the salinity 5 and 30 groups developed tubular systems and abundant cristae mitochondria. The tubular system of the salinity 20 group was non-spatially constant and had loose structure. Part of the tubular system contracted into a pearl bubble structure and shared rough endoplasmic reticulum. Mitochondrion-rich cells in Trachinotus ovatus under salinities 5 and 20 appeared both seawater-type's and freshwater-type's features, and those in salinity 30 had typical characteristics as seawater-type MR cells. Structural changes of mitochondrion-rich cells were suited to different osmotic pressure.
