[Studies on separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin].

OBJECTIVE: To study the technology for separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin. METHODS: The absorption and separation abilities of 10 kinds of adsorption resin were studied and the separation and purification technological process of phenylethanoid glycosides from leaves of Forsythia suspensa was investigated by HPLC with the content of Forsythoside A as an index. RESULTS: The optimal conditions were as follows: taking AB-8 resin column as adsorbent, the resin was washed by 20BV distilled water to remove impurity and 8BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2BV/h, the content of Forsythoside A in the extract was 34.8%. CONCLUSION: The process with AB-8 resin is an effective method to separate and purify phenylethanoid glycosides.

[Studies on chemical constituents of alpinia katsumadai].

OBJECTIVE: To study the chemical constituents from the ethanolic extract of Alpinia katsumadai Hayata. METHODS: The compounds were separated with the column chromatographic, and their structures were identified by chemical and spectroscopic methods. RESULTS: Eight compounds were isolated from the ethanol extract. On the basis of their physical and chemical properties and spectroscopic analysis, the structures of seven compounds were identified. They were 1,7-Diphenyl-5-hydroxy-4, 6-heptadien-3-one(I), 1,7-Diphenyl4, 6-heptadien-3-one(II), Pinocembrin (III), Cardamomin (IV), Alpinetin (V), 7,4'-Dihydroxy-5-methoxy flavanones(VI) and beta-Sitosterol(VIII), respectively. CONCLUSION: Compounds I , II and VII are separated from this plant for the first time. Compounds I and II are also obtained from this genus of Alpinetin for the first time.

[Studies on lignan glycosides from the roots of Bupleurum scorzonerifolium].

AIM: To study the lignan glycosides from the roots of Bupleurum scorzonerifolium. Chromatography methods was used to isolate compounds and chemical and spectral methods were used to identify the structures of isolated compounds. RESULTS: Two lignan glycosides were isolated from the roots of B. scorzonerifolium and their structures were identified as 2, 3-E-2,3-dihydro-2-(3'-methoxy-4'-O-beta-D-glucopyranosyl-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl )-7-methoxy-1-benzo [b] furan (1) and 2, 3-E-2, 3-dihydro-2-( 3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-( 3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b]furan (2), both of them which were the mixture of (+)2S,3R- and (-)2R,3S-type diastereoisomer and in both of them the amount of (+)2S,3R-type was a little more than that of the (-)2R,3S-type. CONCLUSION: Compounds 1 and 2 were isolated from B. scorzonerifolium for the first time. (+)2S, 3R-2, 3-Dihydro-2-(3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b] furan is a new compound.