Role of diatom-derived oxylipins in organic phosphorus recycling during coastal diatom blooms in the northern South China Sea.

Diatom-bacteria interactions and the associated bloom dynamics have not been fully understood in the coastal oceans. Here, we focus on the polyunsaturated aldehydes (PUAs) produced by diatoms in the post-bloom phase and look into their roles in microbial phosphorus (P) recycling outside of a P-limited estuary. The phytoplankton community in the bloom was dominated by PUAs-producing diatoms (Skeletonema costatum, Thalassiosira spp., and Pesudonitzschia delicates) with elevated concentrations of biogenic particulate PUAs. In addition, there were micromolar levels of particle-adsorbed PUAs hotspots with distinct compositions in and out of the bloom determined by a combining large-volume filtration and on-site derivation method. Field experiments were conducted to further assess the responses of particle-attached bacteria (PAB) to different PUAs amendments. We found no differences in the alkaline phosphatase (APase) activity and the abundance of PAB between inside and outside the bloom at a low PUAs dosage (<30 µM). However, for a high PUAs dosage (300 µM), APase activity and PAB growth were reduced significantly outside the bloom but no influences within the bloom. Our findings indicate that the hotspot-level oxylipins may play essential roles in bacterial P-remineralization in P-limited coastal areas. PAB can adapt to the high level of PUAs released by diatoms (or their resulting detritus) and potentially maintain a high rate of organic P recycling during the late stages of diatom blooms. Consequently, the interaction between oxylipin-rich diatoms and bacteria may affect phytoplankton blooms and carbon sequestration in the coastal oceans.

Microplastic pollution and regulating factors in the surface sediment of the Xuande Atolls in the South China Sea.

Microplastics are widely present in the marine environment, but their pollution and potential risk assessment in the seabed sediments have not been well addressed in remote sea areas. In this study, microplastics in 50 surface sediment samples from the Xuande Atolls at the Xisha of the South China Sea were studied. There were 20 samples with detectable microplastics of 5-20 items kg-1. They were all fibers in shapes and blue/transparent in colors with the dominant chemical component of polyester and the typical size of 0.02-3 mm. We found a large spatial variability of microplastic abundance in the surface sediment with generally low or undetectable levels in the lagoon deposits and the offshore deep-sea sediments but elevated abundances in the slope sediments of the Xuande Atolls. Correlation analyses suggested that microplastic variability in the Xisha sediment was less affected by local environmental parameters such as water depth, sediment particle size, organic carbon content, and sediment types. We also found that elevated microplastics in the seabed sediments on various sides of the Xuande Atolls could be related to the seasonal change in monsoon-driven currents. Finally, a low risk of microplastic pollution in the surface sediment of the Xisha is concluded based on the assessments of the polymer hazard index and the pollution load index. These findings provide not only a baseline understanding of microplastics but also their dynamics in the surface sediment of the remote Xisha area of the South China Sea.

Microplastic contamination in coral reef fishes and its potential risks in the remote Xisha areas of the South China Sea.

Microplastics are recognized as a newly emerging threat to marine organisms as they can be ingested and accumulated through multiple trophic levels. However, microplastic contamination and its potential risk assessment in coral reef fishes have been less addressed, particularly in remote ocean regions. In this study, microplastics in 167 samples of coral reef fish (a total of eighteen species) from the Xisha areas of the South China Sea were studied. There were fifteen species of coral reef fish contaminated by microplastics with an average occurrence rate of 29.3 %. The shape of microplastics in the fishes was mostly fibrous with small sizes (400-900 µm) and light colors (transparent and blue). The dominant types of microplastic polymers are polyamide and polyethylene terephthalate, accounting for 77 % and 11 % of microplastics in the fish body. There were generally more microplastics in the herbivorous fishes than the carnivorous ones. The highest microplastic abundance and occurrence was found in parrotfish due to its direct feeding on the microplastics-contaminated corals. In addition, there were much more microplastics in the gastrointestinal tracts than in the gills of the Xisha fishes. Microplastic abundance was found negatively correlated with the trophic level of the Xisha fishes supporting a stronger microplastic impact at lower levels of marine animals. Finally, a risk assessment using the polymer hazard index (PHI) revealed that microplastic contamination in the Xisha fishes was lower than those in the eutrophic coast. Our study provides new evidence for the widespread presence of microplastic contamination in the fishes of the remote Xisha coral reefs.

Transparent exopolymer particle dynamics along a shelf-to-sea gradient and impacts on the regional carbon cycle.

Transparent exopolymer particles (TEPs) have drawn extensive attention in recent decades due to their crucial role in the biogeochemical and ecological processes of the ocean. However, TEP distribution and fluxes are relatively less addressed in the shelf-seas, where its variability can be affected by not only biology but also complex physical dynamics. Here, we present a comprehensive study of TEP from the coast to the basin (12 sampling sites) of the northern South China Sea (NSCS). We found a large TEP variability from 0.6 to 78.6 µg Xeq. L-1 with higher levels in the coastal waters than the offshore epipelagic waters and the deep waters. In addition, the spatial distribution of TEP was significantly correlated to the cross-shelf change of temperature, salinity, and chlorophyll-a, revealing the complex physical-biogeochemical controls on TEP variability. We found the TEP dynamics nearshore largely influenced by the sedimentation and transportation of TEP-rich aggregates from the river plume. The contribution of TEP to particulate organic carbon (POC) increased gradually when approaching the shore from the sea, suggesting an elevated role of TEP in the coastal carbon cycle. Finally, a good correlation of particle-attached bacteria (PAB) with TEP but not POC revealed a preferential utilization of TEP by PAB. Thus, TEP may play an essential role in the recycling of carbon and nitrogen in the shelf-sea. These findings are crucial for understanding of the TEP dynamics under a changing environment and the associated impacts on the oceanic carbon cycle.

Biogeochemical controls of surface ocean phosphate.

Surface ocean phosphate is commonly below the standard analytical detection limits, leading to an incomplete picture of the global variation and biogeochemical role of phosphate. A global compilation of phosphate measured using high-sensitivity methods revealed several previously unrecognized low-phosphate areas and clear regional differences. Both observational climatologies and Earth system models (ESMs) systematically overestimated surface phosphate. Furthermore, ESMs misrepresented the relationships between phosphate, phytoplankton biomass, and primary productivity. Atmospheric iron input and nitrogen fixation are known important controls on surface phosphate, but model simulations showed that differences in the iron-to-macronutrient ratio in the vertical nutrient supply and surface lateral transport are additional drivers of phosphate concentrations. Our study demonstrates the importance of accurately quantifying nutrients for understanding the regulation of ocean ecosystems and biogeochemistry now and under future climate conditions.

Macroecological patterns of the phytoplankton production of polyunsaturated aldehydes.

The polyunsaturated aldehydes (PUAs) are bioactive metabolites commonly released by phytoplankton species. Based primarily on laboratory experiments, PUAs have been implicated in deleterious effects on herbivores and competing phytoplankton species or in the regulation of the rates of bacterial organic matter remineralization; however, the role of the PUAs at an ecosystem level is still under discussion. Using data of PUA production in natural phytoplankton assemblages over a wide range of conditions, we analyzed macroecological patterns aiming for a comprehensive environmental contextualization that will further our understanding of the control and ecologic role played by these compounds. PUA composition changed from the predominance of decadienal in oligotrophy, octadienal in eutrophy, and heptadienal at intermediate conditions. The production of PUAs per unit biomass also showed a strong relationship with the trophic status, sharply increasing towards oligotrophic conditions and with small-sized cells reaching the highest production rates. High ratios of dissolved inorganic nitrogen to dissolved inorganic phosphorus also promoted PUA production, albeit to a considerably lesser extent. Although the allelopathic use of PUAs to outcompete other phytoplankton or reduce herbivory may be key in some environments and interactions, the macroecological patterns found here, showing higher production towards the poorest waters and among the small species typically populating these environments, support and link at the large scale the hypotheses of the nutrient-derived stress as driver for the production of PUAs together with the use of these compounds as boosters for the nutrient remineralization.

Intercomparison and coupling of magnesium-induced co-precipitation and long-path liquid-waveguide capillary cell techniques for trace analysis of phosphate in seawater.

Currently, two common techniques for nanomolar-level phosphate measurements in seawater are magnesium-induced co-precipitation (MAGIC) and long-path liquid-waveguide capillary cell (LWCC) spectrophotometry. These techniques have been applied in the open ocean, and our understanding of phosphate distributions in oligotrophic subtropical gyres is based on those data. However, intercomparison of these methods has not previously been performed at nanomolar levels. Here, we report experimental results directly comparing the MAGIC and LWCC techniques. We also evaluated the impact of various commonly employed filters on phosphate determinations, as well as interferences from dissolved organic phosphorus (DOP) and arsenate. Our results find agreement between these methods at phosphate concentrations <100nM. We found that filter selection is important for accurate determinations of phosphate, and that DOP hydrolysis affects both techniques similarly. Finally, we demonstrate the advantage of combining MAGIC preconcentration and LWCC spectrophotometry for analysis of very low nanomolar concentrations.

Eddy/wind interactions stimulate extraordinary mid-ocean plankton blooms.

Episodic eddy-driven upwelling may supply a significant fraction of the nutrients required to sustain primary productivity of the subtropical ocean. New observations in the northwest Atlantic reveal that, although plankton blooms occur in both cyclones and mode-water eddies, the biological responses differ. Mode-water eddies can generate extraordinary diatom biomass and primary production at depth, relative to the time series near Bermuda. These blooms are sustained by eddy/wind interactions, which amplify the eddy-induced upwelling. In contrast, eddy/wind interactions dampen eddy-induced upwelling in cyclones. Carbon export inferred from oxygen anomalies in eddy cores is one to three times as much as annual new production for the region.