RESUMO
PURPOSE: To investigate the effect and mechanism of matrine on adhesion and invasion of human adenoid cystic carcinoma ACC-M cells in vitro. METHODS: MTT assay was used to examine the effect of matrine on proliferation of ACC-M cells treated after 1 day to 3 days. Byden chamber assay was performed to detect the effect of matrine on invasion capacity of the cells; Effect on adhesion potential of ACC-M cells was tested by cell matrigel adhesion assay. Mechanism of inhibitory adhesion and invasion was investigated by expression of E-cad protein. The results was analyzed by SPSS11.0 software package. RESULTS: Matrine could inhibit the proliferation of ACC-M cell lines with apparent dose-dependent and time-dependent effect. Matrine significantly inhibited adhesion and invasion capacity of ACC-M cell lines in vitro with concentration increasing. The expression level of E-cad was significantly increased compared with the untreated group in ACC-M cell lines. CONCLUSIONS: Matrine can inhibit the adhesion and invasion capacity of ACC-M cell lines in vitro. Mechanism of the inhibition effect may be related to over expression of E-cad protein.
