Screening of phosphorus solubilizing microorganisms in cold environment and their effects on the growth of Brassica napus.

To screen out phosphorus solubilizing strains that can adapt to cold climate in Qinghai Province, Bacillus mucilaginosus, B. megaterium, B. cereus, Streptomyces violovariabilis, S. cinnamofuscus, and S. flavoagglomeratus were screened with solid plate medium as the primary and liquid medium as the secondary screening, with calcium phosphate, lecithin, and phytic acid as the single source of phosphorus. By comprehensively comparing the size of phosphate solubilizing circle in the solid plate medium and the soluble phosphorus content in the liquid medium, three strains of phosphate solubilizing bacteria with good phosphate solubilizing effects were screened, S. violovariabilis, S. cinnamofuscus, and B. mucilaginosus. The three phosphate solubilizing bacteria were made into liquid ino-culants, and the small rapeseed pot experiment was carried out with two soils with different fertilities in a cold climate in September. Compared with the control, plant height, fresh weight, root length, and root weight of rapes in high-fertility cultivated soil increased by 35.5%, 191.0%, 26.2%, and 282.7%, while plant phosphorus absorption, total phosphorus and available phosphorus contents in the rhizosphere soil increased by 968.9%, 5.1%, and 2.1%, respectively. In low-fertility soil, plant height and fresh weight was increased by 45.8% and 61.3%, root length and weight was decreased by 2.6% and 4.4%, while plant phosphorus absorption and the contents of total P and available P in rhizosphere soil were increased by 91.5 %, 29.1%, and 213.7%, respectively. The effect of the other two inoculants treatments was less significant than S. violovariabilis. Therefore, S. violovariabilis was the phosphate solubilizing strain suitable for the cold climate in Qinghai.

[Investigation on anopheline species in Chayu County, Linzhi Prefecture of Tibet Autonomous Region].

OBJECTIVE: To investigate Anopheline species in Chayu County of Linzhi Prefecture, Tibet. METHODS: Four natural villages in Chayu County were selected in this study in 2010. The methods of overnight/semi-overnight trapping indoor and outdoor human-bait, and overnight trapping with light traps were used, and all the Anopheline mosquitoes were confirmed by morphological characteristics. RESULTS: Totally 2 991 Anopheline mosquitoes were captured, with 76.36% (2 284/2 291) of An. peditaeniatus, 22.30% (667/2 291) of An. maculates group. The average densities of An. peditaeniatus by semi-overnight trapping were 56.2/per person indoor and 4/per person outdoor respectively, and the average densities of An. maculatus group by semi-overnight trapping were 17.8/per person indoor and 17.9/per person outdoor respectively. The man-biting rates of An. peditaeniatus by overnight trapping were 28.1/per person indoor and 2/per person outdoor respectively, and were both 8.9/per person to An. maculatus group. CONCLUSION: An. peditaeniatus and An. maculatus group are the possible transmission vectors of malaria in Chayu County.

[The clinical application on composite graft of acellular allo-dermal matrix and auto-microskin on escharectomy after deep burns].

OBJECTIVE: To present the clinical application of composite graft of acellular allo-dermis matrix (ADM) with thin auto-microskin on burn wound. METHODS: 8 inpatients with 18 full thickness skin burn wounds were transplanted with allo-ADM after eschar was excised, then the auto-microskin and allo-human skin were covered on the area of the matrix, the wound where no allo-ADM grafting were covered as control groups only with auto-microskin and allo-human skin. The area of donor to wound is 1:5 - 1:8. RESULTS: Survived rate of 18 pieces composite skin that allo-ADM with auto-microskin were grafted were 94%. After following up for 3 to 13 months, the skins of complex grifting had well elastic and smooth texture compared to auto-microskin grafted, they appeared less cicatrisation and ulceration. 3 months after operation, it was indicated by histological examination that tightknit the epithelial-dermal conjunction and epidermal papilla structure could be identified in the allo-ADM skin and there were orderly collagenous fibres, but scar skin structure was observed in that auto-microskin grifted area. CONCLUSION: The graft effectiveness of allo-ADM and auto-microskin was better than that of auto-microskin, and this method could be used on major deep burn wound healing.