Application of Bioinformatics in Predicting the Efficacy of Digestive Tumour Immunotherapy Target TIM-3 and its Inhibitors.

Background: Our main objective is to apply bioinformatics in predicting the efficacy of digestive tumour immunotherapy target TIM-3 and its inhibitors. Methods: Our study used the gene expression omnibus (GEO) database to identify datasets associated with digestive tumours and the action of TIM-3. The GSE427729 dataset based on the GPL10192 platform. The dataset consisted of six samples of total RNA derived from TIM-3 control and knockdown RAW 264.7 cells. We used GEO2R tool to identify DEGs before performing Gene Ontology and identifying the kyoto encyclopedia of genes and genomes (KEGG) pathways. Lastly, we determined the PPI networks to identify hub genes. Results: Our study identified 57 differentially expressed genes based on an adjusted p-value of less than 0.05 and a log2 fold change of 2.0. There were 26 down-regulated genes with 31 up-regulated genes while 22, 404 genes were non-significant. The DEGs were enriched in biological pathways such as activating leukocytes, cells, and development of the immune system. Additionally, we identified four significant KEGG pathways that were implicated in digestive tumour immunotherapy and TIM-3; pathways of pancreatic cancer, NF-Kappa B signalling pathway, Toll-like receptor signalling pathway and C-type lectin receptor signalling pathway. The PPI networks identified 10 hub genes that were implicated in digestive tumour immunotherapy target TIM-3 (Myd88, Traf6, Irf7, Cdk4, Ccnd2, Mapkap1, Prr5, Mpp3, Serpinb6b and Pvrl3). Conclusion: Targeting these biological pathways, KEGG pathways, molecular functions and cellular processes can lead to novel therapeutic treatment and management in digestive tumours based on TIM-3 immunotherapy.

Phase diagram of chiral magnets via Green's function method.

In this paper, Green's function method is applied to study the ferromagnetic system with Dzyaloshinkii-Moriya (DM) interaction in both two-dimension (2D) and three-dimension chiral magnets. Relevant properties in 2D magnets are calculated, such as the susceptibility, correlation function and analytical expressions of phase boundary. Based on the theoretical results, a new phase is predicted in the window of strong DM interaction characterized by a negative winding number. In addition, helical state in pure 2D material only appears at zero temperature. The analysis on correlation function shows a special symmetry of transverse spin correlation, which corresponds to the skyrmion phase. The results also prove the instability of helical state and its lifetime is numerically computed. Different from 2D magnets, helical state in 3D exists in the window of a lower Zeeman energy and has a long lifetime. DM interaction also reduces Curie temperature because of the spatial symmetry breaking.

Ultra-Broadband Nonlinearity Enhancement based on a Novel Graphene-Silicon Hybrid Waveguide: Structure Design and Theoretical Analysis.

A graphene-silicon hybrid waveguide with a dielectric spacer is proposed to enhance the nonlinear response in ultra-wide wavelength range by applying graphene's broadband highly nonlinear optical properties. The chemical potential of the graphene layer is tuned to satisfy the resonance condition and hence a low propagation loss is obtained. The dielectric spacer is used for avoiding additional free-carrier-absorption loss due to carrier interchange between the silicon core and the graphene layer. Aiming at the special waveguide structure with ultra-thin graphene layer, a full-vectorial theoretical model is developed to analyze its nonlinear properties. The waveguide dimensions are optimized in terms of the nonlinear parameter. The proposed hybrid waveguide exhibits high nonlinearity enhancement in an ultra-broad wavelength region covering near-infrared and mid-infrared bands. The conversion efficiency for a degenerate four-wave mixing process reaches -18.5 dB only with a pump power of 0.5 W and a waveguide length of tens of microns. In the wavelength range of 1.3-2.3 µm, the conversion efficiency can be kept stable by adopting suitable waveguide geometry and length. The corresponding 3-dB bandwidth can reach 40-110 nm for each fixed pump. The graphene-silicon hybrid waveguide has the potential to support chip-scale nonlinear applications in both near- and mid-infrared bands.

[Analysis on related factors of perioperative total blood loss in unilateral total hip arthroplasty].

OBJECTIVE: To investigate the influential factors of total blood loss during unilateral artificial total hip arthroplasty (THA). METHODS: From January 2014 to July 2016, 131 patients undergwent primary unilateral total hip arthroplasty, including 55 males and 76 females, ranging in age from 40 to 89 years old, with an average of 64.5 years old. The data of patients with unilateral total hip arthroplasty were collected, and the data of hemodynamics, coagulation function, hemoglobin and hematocrit values were recorded according to the observation data. The blood loss of the patients was calculated by Gross equation, and the data were analyzed by a statistical software. RESULTS: The total blood loss of the patients was significantly affected by the factors of pathogenesis and deep venous thrombosis of the lower limbs. The results were statistically significant(P<0.05). The gender, age, body mass index, prosthesis and anesthesia had little effects on the blood loss volume after replacement. CONCLUSIONS: The data in this study indicate that the volume of actual blood loss can be controlled by adjusting non-surgical factors such as gender, age, body mass index, prosthetic material, and risk factors, and surgical factors(postoperative complications, anesthesia).