Multi-Modal Melt-Processing of Birefringent Cellulosic Materials for Eco-Friendly Anti-Counterfeiting.

Ubiquitous anti-counterfeiting materials with a rapidly rising annual consumption (over 1010 m2) can pose a serious environmental burden. Biobased cellulosic materials with birefringence offer attractive sustainable alternatives, but their scalable solvent-free processing remain challenging. Here, a dynamic chemical modification strategy is proposed for multi-modal melt-processing of birefringent cellulosic materials for eco-friendly anti-counterfeiting. Relying on the thermal-activated dynamic covalent-locking of the spatial topological structure of preferred oriented cellulose, the strategy balances the contradiction between the strong confinement of long-range ordered structures and the molecular motility required for entropically-driven reconstruction. Equipped with customizable processing forms including mold-pressing, spinning, direct-ink-writing, and blade-coating, the materials exhibit a wide color gamut, self-healing efficiency (94.5%), recyclability, and biodegradability. Moreover, the diversified flexible elements facilitate scalable fabrication and compatibility with universal processing techniques, thereby enabling versatile and programmable anti-counterfeiting. The strategy is expected to provide references for multi-modal melt-processing of cellulose and promote sustainable innovation in the anti-counterfeiting industry.

Membrane protein MHZ3 regulates the on-off switch of ethylene signaling in rice.

Ethylene regulates plant growth, development, and stress adaptation. However, the early signaling events following ethylene perception, particularly in the regulation of ethylene receptor/CTRs (CONSTITUTIVE TRIPLE RESPONSE) complex, remains less understood. Here, utilizing the rapid phospho-shift of rice OsCTR2 in response to ethylene as a sensitive readout for signal activation, we revealed that MHZ3, previously identified as a stabilizer of ETHYLENE INSENSITIVE 2 (OsEIN2), is crucial for maintaining OsCTR2 phosphorylation. Genetically, both functional MHZ3 and ethylene receptors prove essential for OsCTR2 phosphorylation. MHZ3 physically interacts with both subfamily I and II ethylene receptors, e.g., OsERS2 and OsETR2 respectively, stabilizing their association with OsCTR2 and thereby maintaining OsCTR2 activity. Ethylene treatment disrupts the interactions within the protein complex MHZ3/receptors/OsCTR2, reducing OsCTR2 phosphorylation and initiating downstream signaling. Our study unveils the dual role of MHZ3 in fine-tuning ethylene signaling activation, providing insights into the initial stages of the ethylene signaling cascade.

In-plane compressive responses and failure behaviors of composite sandwich plates with resin reinforced foam core.

The paper presented an experimental study on the effect of the resin reinforced core configuration and core thickness on in-plane compressive responses and failure behaviors of composite sandwich specimens. Two resin reinforced core machining configurations were designed with three core thickness along. In-plane compressive load, displacement, strains on both sides, and failure morphology were closely monitored during the loading process. Meanwhile, the theoretical method also was supplementary to forecast the failures of sandwich structures. It was found that the enhancement of grooved, perforated holes and contour cut (GPC) core was better than double-side grooved and perforated hole (DGP) core to improve the in-plane compressive capacity of sandwich specimens for all thick cores. The core fracture or skin/core debonding failure of sandwich specimens resulted in an instant drop of in-plane compressive load, and the global buckling led to a slower reduction. The failure mode changed from global buckling to skin/core debonding at both sides as the core thickness increased for the Plain core sandwich specimen; switched from global buckling to a combined failure of core fracture and skin/core debonding at both sides, and then to skin/core debonding at both sides for the DGP core sandwich specimen; the skin/core debonding at the shallow side occurred for all GPC core specimens. The slight buckling trace of strains before the peak load probably triggered the skin/core debonding of sandwich specimens. The theoretical method could well forecast failure loads and corresponding failure modes of sandwich specimens with the 15 mm thick core, and reasonably predict failure loads for sandwich specimens with 30 mm and 45 mm thick cores.

Mineral oil emulsion species and concentration prediction using multi-output neural network based on fluorescence spectra in the solar-blind UV band.

The accurate monitoring of oil spills is crucial for effective oil spill recovery, volume determination, and cleanup. Oil slicks become emulsified under the effects of wind and waves, which increases the consistency of the oil spills. This phenomenon makes oil spills more challenging to handle and exacerbates environmental pollution. In this study, the variation of the solar-blind ultraviolet (UV) fluorescence spectra obtained from simulated oil spills with different oil types and oil-water ratios was investigated. By designing and constructing a multi-angle excitation and detection system, an apparent fluorescence peak of the oil emulsions was observed at around 290 nm under 220 nm excitation. By utilizing competitive adaptive reweighted sampling (CARS) and multi-output neural network algorithms, both the types and concentrations of the emulsified oils were obtained simultaneously. The classification accuracy for identifying the oil type exceeds 98%, and the mean absolute percentage error (MAPE) for concentration regression is around 2%. The results indicate that active solar-blind UV fluorescence could become a supplementary method for on-site oil spill detection to achieve comprehensive monitoring of oil spills. This study provides potential applications for UV-induced fluorescence spectrometry in oil spill on-site monitoring during the daytime.

An RRM domain protein SOE suppresses transgene silencing in rice.

Gene expression is regulated at multiple levels, including RNA processing and DNA methylation/demethylation. How these regulations are controlled remains unclear. Here, through analysis of a suppressor for the OsEIN2 over-expressor, we identified an RNA recognition motif protein SUPPRESSOR OF EIN2 (SOE). SOE is localized in nuclear speckles and interacts with several components of the spliceosome. We find SOE associates with hundreds of targets and directly binds to a DNA glycosylase gene DNG701 pre-mRNA for efficient splicing and stabilization, allowing for subsequent DNG701-mediated DNA demethylation of the transgene promoter for proper gene expression. The V81M substitution in the suppressor mutant protein mSOE impaired its protein stability and binding activity to DNG701 pre-mRNA, leading to transgene silencing. SOE mutation enhances grain size and yield. Haplotype analysis in c. 3000 rice accessions reveals that the haplotype 1 (Hap 1) promoter is associated with high 1000-grain weight, and most of the japonica accessions, but not indica ones, have the Hap 1 elite allele. Our study discovers a novel mechanism for the regulation of gene expression and provides an elite allele for the promotion of yield potentials in rice.

Dynamic Covalent Bonded Gradient Structured Actuators with Mechanical Robustness and Self-Healing Ability.

Flexible actuators with excellent adaptability and interaction safety have a wide range of application prospects in many fields. However, current flexible actuators have problems such as fragility and poor actuating ability. Here, inspired by the features of nacre structure, a gradient structured flexible actuator is proposed with mechanical robustness and self-healing ability. By introducing dynamic boronic ester bonds at the interface between MXene nanosheets and epoxy natural rubber matrix, the resulting nanocomposites with ordered micro-nano structures exhibit excellent tensile strength (25.03 MPa) and satisfactory repair efficiency (81.2%). In addition, the gradient distribution structure of MXene nanosheets endows the actuator with stable photothermal conversion capability, which can quickly respond to near-infrared light stimulation. The interlayer dynamic covalent bond crosslinking enables good response speed after multiple bending and is capable of functional self-healing after damage. This work introduces gradient structure and dynamic covalent bonding into flexible actuators, which provides a reference for the fabrication of self-healing soft robots, wearable, and other healable functional materials.

Graph neural network recommendation algorithm based on improved dual tower model.

In this era of information explosion, recommendation systems play a key role in helping users to uncover content of interest among massive amounts of information. Pursuing a breadth of recall while maintaining accuracy is a core challenge for current recommendation systems. In this paper, we propose a new recommendation algorithm model, the interactive higher-order dual tower (IHDT), which improves current models by adding interactivity and higher-order feature learning between the dual tower neural networks. A heterogeneous graph is constructed containing different types of nodes, such as users, items, and attributes, extracting richer feature representations through meta-paths. To achieve feature interaction, an interactive learning mechanism is introduced to inject relevant features between the user and project towers. Additionally, this method utilizes graph convolutional networks for higher-order feature learning, pooling the node embeddings of the twin towers to obtain enhanced end-user and item representations. IHDT was evaluated on the MovieLens dataset and outperformed multiple baseline methods. Ablation experiments verified the contribution of interactive learning and high-order GCN components.

A translational regulator MHZ9 modulates ethylene signaling in rice.

Ethylene plays essential roles in rice growth, development and stress adaptation. Translational control of ethylene signaling remains unclear in rice. Here, through analysis of an ethylene-response mutant mhz9, we identified a glycine-tyrosine-phenylalanine (GYF) domain protein MHZ9, which positively regulates ethylene signaling at translational level in rice. MHZ9 is localized in RNA processing bodies. The C-terminal domain of MHZ9 interacts with OsEIN2, a central regulator of rice ethylene signaling, and the N-terminal domain directly binds to the OsEBF1/2 mRNAs for translational inhibition, allowing accumulation of transcription factor OsEIL1 to activate the downstream signaling. RNA-IP seq and CLIP-seq analyses reveal that MHZ9 associates with hundreds of RNAs. Ribo-seq analysis indicates that MHZ9 is required for the regulation of ~ 90% of genes translationally affected by ethylene. Our study identifies a translational regulator MHZ9, which mediates translational regulation of genes in response to ethylene, facilitating stress adaptation and trait improvement in rice.

Biobased Intelligent Food-Packaging Materials with Sustained-Release Antibacterial and Real-Time Monitoring Ability.

It has been widely accepted that sustainable polymers derived from renewable resources are able to replace the short-turnover petroleum-based materials and reduce environmental impact in the future. However, their hydrophilic chemical structures rich with oxygen groups could lead to easy growth of bacteria, which greatly limit their applications in packaging materials. Here, we present an intelligent food-packaging material with sustained-release antibacterial and real-time monitoring ability based on totally biobased contents. In detail, sodium alginate with Artemisia argyi emission oil (encapsulated in gelatin-Arabic gum microcapsules) and citric acid-sourced pH-responsive carbon quantum dots (CQDs) are coated on bamboo cellulose papers. The obtained biobased composite material (almost 100% biocarbon content) with antibacterial ability is able to extend the shelf life of fresh shrimps and can be biodegraded. Moreover, owing to the introduction of CQDs, the composite can rapidly (within 1 s) detect slight pH variations (response pH ∼5, 10-9 mol/L of OH-) through an obvious color change (hue value from 305 to 355°). The developed strategy may open up new opportunities in the design of multifunctional biobased composites for intelligent applications.

Far ultraviolet mirrors for aurora imaging: design and fabrication.

The emission lines of 140-180 nm are auroral bands of N 2 Lyman-Birge-Hopfield, and they have been imaging targets of many satellites that need reflective mirrors. To obtain good imaging quality, the mirrors also should have excellent out-of-band reflection suppression as well as high reflectance at working wavelengths. We designed and fabricated non-periodic multilayer L a F 3/M g F 2 mirrors with working wave bands of 140-160 nm and 160-180 nm, respectively. We used a match design method and deep search method to design the multilayer. Our work has been utilized in the new wide-field auroral imager of China, and the application of these notch mirrors with excellent out-of-band suppression reduces the utilization of corresponding transmissive filters in the optical system of space payload. Furthermore, our work provides new routes for the design of other reflective mirrors in the far ultraviolet region.

Pinholes in Al/MgF2.

Pinholes in A l/M g F 2 were first inferred by the transmittance enhancement in Larruquert group's research. However, no direct proof was reported to verify the existence of the pinholes in A l/M g F 2. In the past 80 years, pinholes were first observed by dark-field microscopy and bright-field microscopy in the transmission mode. They were small and on the order of several hundred nanometers to several micrometers. Essentially, the pinhole was not a real hole, partially because of the lack of the Al element. Increasing the thickness of Al cannot reduce the size of the pinholes. The occurrence of the pinholes was dependent on the deposition rate of the Al film and the substrate heating temperature, and it was independent of the substrate materials. This research eliminates an otherwise easily ignored scattering source, and it will benefit the development of ultra-precise optics, including mirrors for gyro-lasers, the detection of gravitational waves, and coronagraph detection.

Detection of the intraoperative bile leakage in laparoscopic cholecystectomy using indocyanine green fluorescence imaging: A case report.

Herein, we report two cases of successful application of indocyanine green (ICG) fluorescence imaging for detecting intraoperative bile leakage during laparoscopic cholecystectomy (LC). Bile leakage was detected rapidly and accurately using fluorescence guidance. Based on our findings, we recommend using ICG fluorescence imaging during LC because it is effective and feasible for detecting intraoperative bile leakage.

Ultraviolet-induced fluorescence of oil spill recognition using a semi-supervised algorithm based on thickness and mixing proportion-emission matrices.

In recent years, marine oil spill accidents have been occurring frequently during extraction and transportation, and seriously damage the ecological balance. Accurate monitoring of oil spills plays a vital role in estimating oil spill volume, determination of liability, and clean-up. The oil that leaks into natural environments is not a single type of oil, but a mixture of various oil products, and the oil film thickness on the sea surface is uneven under the influence of wind and waves. Increasing the mixed oil film thickness dimension and the mix proportion dimension has been proposed to weaken the effect of the detection environment on the fluorescence measurement results. To preserve the relationships between the data of oil films with different thicknesses and the relationships between the data of oil films with different mixing proportions, the three-dimensional fluorescence spectral data of mixed oil films on a seawater surface were measured in the laboratory, producing a thickness-fluorescence matrix and a proportion-fluorescence matrix. The nonlinear variation of the fluorescence spectra was investigated according to the fluorescence lidar equation. This work pre-processes the data by sum normalization and two-dimensional principal component analysis (2DPCA) and uses the dimensionality reduction results as two feature-point views. Then, semi-supervised classification of collaborative training (co-training) with K-nearest neighbors (KNN) and a decision tree (DT) is used to identify the samples. The results show that the average overall accuracy of this coupling model can reach 100%, which is 20.49% higher than that of the thickness-only view. Using unlabeled data can reduce the cost of data acquisition, improve the classification accuracy and generalization ability, and provide theoretical significance and application prospects for discrimination of spectrally similar oil species in natural marine environments.

Unbalanced Glutamine Partitioning between CD8T Cells and Cancer Cells Accompanied by Immune Cell Dysfunction in Hepatocellular Carcinoma.

Glutamine metabolism is critical both for the proliferation of cancer cells and the activation of CD8T cells to kill cancer cells. We aim to explore the relationship between the glutamine metabolism of CD8T cells and cancer cells and tumor immunity in the tumor microenvironment. In a TCGA cohort, we found that patients with high scores of glutamine-metabolism-related genes showed poor prognoses, and that a high score of glutamine-metabolism-related genes was an independent risk factor for HCC patients. In single-cell RNA-seq data, we found that, in some patients, the glutamine metabolism gene scores of tumor cells were significantly higher than those of CD8T cells, while decreased ratios of CD8-Tef-GZMA and suppressed tumor-killing activity of CD8-Tef-APOC2 were observed. A further genetic dynamics pseudotime analysis suggested that immune remodeling of these two subpopulations was accompanied by metabolic reprogramming. CD8-Tef-APOC2 in the dominant group tended to metabolize exogenous lipids, while the metabolic program of CD8-Tef-GZMA in the nondominant group was characterized by amino acid and endogenous lipid synthesis. In addition, we found that the glutamine metabolism inhibitor JHU083 promoted the proliferation of CD8T cells and improved the efficacy of PD-1 blockers. We proposed a new tool to quantify the glutamine partitioning between tumor cells and CD8T cells, through which the unique immune microenvironment could be identified at the transcriptome level. Furthermore, the simultaneous destruction of the glutamine metabolism in tumor cells and CD8T cells facilitated the enrichment of tumor-infiltrating CD8T cells and enhanced the efficacy of immunotherapy.

Estimation precision for a normalized response matrix in linear polarization calibration.

The purpose of polarization calibration is to obtain the response matrix of an instrument such that the subsequent observation data can be corrected. The calibration precision, however, is partially restricted by the noise of the detector. We investigate the precision of the normalized response matrix in the presence of signal-independent additive noise or signal-dependent Poisson shot noise. The influences of the source intensity, type of noise, and calibration configuration on the precision are analyzed. We compare the theoretical model and the experimental measurements of the polarization calibration to show that the relative difference between the two is less than 16%. From this result, we can use the model to determine the minimum source intensity and choose the optimal configurations that provide the required precision.

Successful application of indocyanine green fluorescent imaging for the non-invasive detection of postoperative bile leakage.

There is a lack of a simple and reliable method for the diagnosis of postoperative bile leakage. ICG is a water-soluble fluorescent contrast agent. After intravenous injection of ICG, ICG is eventually excreted only through the biliary tract system into the intestines. It is highly sensitive, stable and nonradioactive, and these characteristics provide a theoretical basis for using ICG to diagnose postoperative bile leakage. Two patients with postoperative bile leakage were successfully identified using indocyanine green fluorescent imaging for the first time, suggesting its potential to be a convenient and reliable method for the diagnosis of postoperative bile leakage.

Immune checkpoint inhibitor-induced colitis complicated with inflammatory intestinal obstruction: a case report and literature review.

Background: The combination therapy using anti-angiogenic drugs and immune checkpoint inhibitors (ICIs) has an excellent safety profile and manageable toxicity. However, ICI therapy may lead to a variety of autoimmune events, known as immune-related adverse events (irAEs), which some secondary complications may occur, such as immune-mediated colitis (IMC) and secondary inflammatory intestinal obstruction. It could impact clinical assessments and treatment decisions. Although there are few reports about secondary inflammatory intestinal obstruction related IMC. Case Description: We report an adult patient who suffered from primary liver cancer, who accepted ICIs (sintilimab) combined with vascular endothelial growth factor (VEGF) inhibitor (bevacizumab). He suffered worsening diarrhea about 5 days following immunotherapy. Computed tomography (CT) revealed the thickening intestinal wall of colon and rectum and massive cerebral gas in the proximal colon cavity. He was hospitalized with a diagnosis of IMC and inflammatory intestinal obstruction. Finally, he underwent the therapeutic option using combined glucocorticoid and somatostatin. His symptoms eased within 3 weeks and he was discharged from the hospital. Conclusions: IMC with diarrhea as the main manifestation requires early diagnosis and timely treatment. If the condition progresses, inflammatory intestinal obstruction may occur and this is a life-threatening situation. It is effective to accept early glucocorticoid and somatostatin while casual surgery may aggravate intestinal inflammation and injury, even death.

PROSPECT-PMP+: Simultaneous Retrievals of Chlorophyll a and b, Carotenoids and Anthocyanins in the Leaf Optical Properties Model.

The PROSPECT leaf optical radiative transfer models, including PROSPECT-MP, have addressed the contributions of multiple photosynthetic pigments (chlorophyll a and b, and carotenoids) to leaf optical properties, but photo-protective pigment (anthocyanins), another important indicator of vegetation physiological and ecological functions, has not been simultaneously combined within a leaf optical model. Here, we present a new calibration and validation of PROSPECT-MP+ that separates the contributions of multiple photosynthetic and photo-protective pigments to leaf spectrum in the 400-800 nm range using a new empirical dataset that contains multiple photosynthetic and photo-protective pigments (LOPEX_ZJU dataset). We first provide multiple distinct in vivo individual photosynthetic and photo-protective pigment absorption coefficients and leaf average refractive index of the leaf interior using the LOPEX_ZJU dataset. Then, we evaluate the capabilities of PROSPECT-MP+ for forward modelling of leaf directional hemispherical reflectance and transmittance spectra and for retrieval of pigment concentrations by model inversion. The main result of this study is that the absorption coefficients of chlorophyll a and b, carotenoids, and anthocyanins display the physical principles of absorption spectra. Moreover, the validation result of this study demonstrates the potential of PROSPECT-MP+ for improving capabilities in remote sensing of leaf photosynthetic pigments (chlorophyll a and b, and carotenoids) and photo-protective pigment (anthocyanins).