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1.
Sci Rep ; 14(1): 14789, 2024 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-38926510

RESUMO

To analyse the genetic aetiology of a child with oculocutaneous albinism and to explore the effects of two mutation sites on the function of the OCA2 protein at the mRNA and protein levels via the use of recombinant carriers in vitro. Whole-exome sequencing (WES) and Sanger sequencing were used to analyse the pathogenic genes of the child and validate the mutations in the parents. pEGFP and phage vectors carrying wild-type and mutant OCA2 were constructed using the coding DNA sequence (CDS) of the whole gene-synthesized OCA2 as a template and transfected into HEK293T cells, after which expression analysis was performed. The child in this study was born with white skin, hair, eyelashes, and eyebrows and exhibited nystagmus. Genetic analysis indicated that the child carried two heterozygous mutations: c.1079C > T (p.Ser360Phe) of maternal origin and c.1095_1103delAGCACTGGC (p.Ala366_Ala368del) of paternal origin, conforming to an autosomal recessive inheritance pattern. In vitro analysis showed that the expression of the c.1079C > T (p.Ser360Phe) mutant did not significantly change at the mRNA level but did increase at the protein level, suggesting that the mutation may lead to enhanced protein stability, and the c.1095_1103delAGCACTGGC (p.Ala366_Ala368del) mutation resulted in the loss of three amino acids in exon 10, producing a truncated protein. In vitro expression analysis also revealed that the expression of the mutant gene was significantly downregulated at both the mRNA and protein levels, suggesting that the mutation can simultaneously produce truncated proteins and lead to protein degradation. This case study enriches the phenotypic spectrum of OCA2 gene disease. In vitro expression analysis confirmed that both mutations affect protein expression, providing a theoretical basis for analysing the pathogenicity of these two mutations.


Assuntos
Albinismo Oculocutâneo , Proteínas de Membrana Transportadoras , Mutação , Humanos , Células HEK293 , Albinismo Oculocutâneo/genética , Proteínas de Membrana Transportadoras/genética , Sequenciamento do Exoma , Feminino , Masculino , Linhagem , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
2.
Curr Med Sci ; 43(6): 1075-1083, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38041791

RESUMO

Endometrial receptivity is an important factor that influences embryo implantation. Thus, it is important to identify an applicable approach to improve endometrial receptivity in women undergoing assisted reproductive technology. Recently, growing evidence has indicated that intrauterine platelet-rich plasma (PRP) infusion is an effective method to obtain a satisfactory reproductive outcome by increasing endometrial thickness and improving endometrial receptivity. Therefore, the present review aims to outline the possible mechanisms of PRP on endometrial receptivity and summarize the present literature on the effects of PRP therapy in improving endometrial receptivity.


Assuntos
Transferência Embrionária , Plasma Rico em Plaquetas , Humanos , Feminino , Implantação do Embrião , Endométrio , Técnicas de Reprodução Assistida
3.
Chin J Nat Med ; 18(8): 594-605, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32768166

RESUMO

To ensure the safety of medications, it is vital to accurately authenticate species of the Apocynaceae family, which is rich in poisonous medicinal plants. We identified Apocynaceae species by using nuclear internal transcribed spacer 2 (ITS2) and psbA-trnH based on experimental data. The identification ability of ITS2 and psbA-trnH was assessed using specific genetic divergence, BLAST1, and neighbor-joining trees. For DNA barcoding, ITS2 and psbA-trnH regions of 122 plant samples of 31 species from 19 genera in the Apocynaceae family were amplified. The PCR amplification for ITS2 and psbA-trnH sequences was 100%. The sequencing success rates for ITS2 and psbA-trnH sequences were 81% and 61%, respectively. Additional data involved 53 sequences of the ITS2 region and 38 sequences of the psbA-trnH region were downloaded from GenBank. Moreover, the analysis showed that the inter-specific divergence of Apocynaceae species was greater than its intra-specific variations. The results indicated that, using the BLAST1 method, ITS2 showed a high identification efficiency of 97% and 100% of the samples at the species and genus levels, respectively, via BLAST1, and psbA-trnH successfully identified 95% and 100% of the samples at the species and genus levels, respectively. The barcode combination of ITS2/psbA-trnH successfully identified 98% and 100% of samples at the species and genus levels, respectively. Subsequently, the neighbor joining tree method also showed that barcode ITS2 and psbA-trnH could distinguish among the species within the Apocynaceae family. ITS2 is a core barcode and psbA-trnH is a supplementary barcode for identifying species in the Apocynaceae family. These results will help to improve DNA barcoding reference databases for herbal drugs and other herbal raw materials.


Assuntos
Apocynaceae/classificação , Código de Barras de DNA Taxonômico , DNA de Plantas/genética , DNA Espaçador Ribossômico/genética , Plantas Medicinais/classificação , Apocynaceae/genética , China , Folhas de Planta , Plantas Medicinais/genética
4.
BMC Pregnancy Childbirth ; 20(1): 433, 2020 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-32727413

RESUMO

BACKGROUND: Twin growth discordance was demonstrated to be a risk factor for adverse perinatal outcomes, and prenatal ultrasonographic methods were utilized to predict twin growth discordance to improve outcomes. The results currently reported are not consistent due to the poor unified parameters and gestational durations. METHODS: A total of 71 dichorionic twins with growth discordance and 346 dichorionic twins with normal growth were respectively included in the retrospective cohort study. The weight discordance of more than 25% was defined as a "growth discordant twin". The clinical baseline information, maternal outcomes, twin birth weights and fetal growth parameters (which were measured by ultrasound) were compared between the two groups from early gestation to late gestation. Multiple logistic regression and receiver operating characteristic curves were adopted to evaluate the predictive value of the growth parameters. RESULTS: Compared with normal dichorionic twins, the clinical baseline conditions were similar in the twins those finally developed growth discordance. The fetal growth parameters and the deepest volume pocket of amniotic fluid in early and mid-pregnancy showed no obvious predictive values. The fetal growth parameters in late pregnancy showed predictive values, especially the discordance of estimated fetal weight (EFW) in the early third-trimester (P < 0.001, area under the curve, 0.822; the cut-off value, 20%; sensitivity, 66.67%; specificity, 91.30%; positive predictive value, 88.46%). CONCLUSION: Two assessment approaches were suggested and adopted to predict twin growth discordance in the current study. Twin growth should be assessed longitudinally and dynamically. Normal twins may show growth imbalance in the early stage. The discordance of EFW in late pregnancy may be a useful indicator for a growth discordance of more than 25%, which is required further confirmation.


Assuntos
Doenças em Gêmeos , Retardo do Crescimento Fetal/diagnóstico por imagem , Gêmeos Dizigóticos , Ultrassonografia Pré-Natal , Peso ao Nascer , China/epidemiologia , Estatura Cabeça-Cóccix , Feminino , Desenvolvimento Fetal , Retardo do Crescimento Fetal/epidemiologia , Peso Fetal , Idade Gestacional , Humanos , Valor Preditivo dos Testes , Gravidez , Trimestres da Gravidez , Gravidez de Gêmeos , Curva ROC , Estudos Retrospectivos
5.
Curr Med Sci ; 39(2): 278-284, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31016522

RESUMO

This observational study included 21 patients at remarkably high risk of ovarian hyperstimulation syndrome (OHSS), characterized by more than 30 follicles measuring ≥11 mm in diameter on trigger day and/or pre-trigger peak estradiol exceeding 10 000 pg/mL, which was also the feature of women with established severe early OHSS followed by gonadotrophin-releasing hormone agonist (GnRHa) trigger and freeze-all policy that previously have been reported. All patients received a second dose of GnRHa 12 h after the first GnRHa trigger combined with administration of GnRH antagonist at 0.25 mg/day for a period of 3 days from the day of oocyte retrieval onwards. The in vitro fertilization (IVF) outcomes may be preferable compared with a bolus of GnRHa trigger and none of the included patients developed moderate-to-severe OHSS. Moreover, patients' symptoms, reproductive hormone levels and ultrasound findings were improved significantly. This new strategy seems to be efficacious and could be a further supplement of GnRHa trigger with or without applying freeze-all strategy to completely prevent early-onset moderate to severe OHSS, especially for the patients characterized by ≤30 follicles measuring ≥11 mm in diameter on trigger day and/or pre-trigger peak estradiol exceeding 10 000 pg/mL. Further studies should be performed to compare this regimen with conventional methods of OHSS prevention.


Assuntos
Estradiol/metabolismo , Fármacos para a Fertilidade Feminina/administração & dosagem , Hormônio Liberador de Gonadotropina/análogos & derivados , Hormônio Liberador de Gonadotropina/agonistas , Folículo Ovariano/efeitos dos fármacos , Síndrome de Hiperestimulação Ovariana/prevenção & controle , Adulto , Feminino , Fertilização in vitro/métodos , Hormônio Liberador de Gonadotropina/administração & dosagem , Humanos , Infertilidade Feminina/tratamento farmacológico , Infertilidade Feminina/metabolismo , Recuperação de Oócitos/métodos , Indução da Ovulação/métodos , Gravidez , Taxa de Gravidez
6.
Nan Fang Yi Ke Da Xue Xue Bao ; 37(10): 1418-1422, 2017 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-29070478

RESUMO

OBJECTIVE: We report a case of ovarian function fluctuation during long-term follow-up in a patient with premature ovarian insufficiency (POI). The patient finally obtained clinical pregnancy with subsequent uneventful full-term delivery after several intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycles. This case demonstrates that hormone replacement therapy (HRT) and assisted reproductive therapy should be applied as soon as possible to young patients with POI who have a strong desire for pregnancy in the absence of contraindications. This strategy helps such patients obtain pregnancy and delivery before the exhaustion of ovarian function.


Assuntos
Transferência Embrionária , Insuficiência Ovariana Primária/terapia , Injeções de Esperma Intracitoplásmicas , Feminino , Terapia de Reposição Hormonal , Humanos , Gravidez , Taxa de Gravidez
7.
Nan Fang Yi Ke Da Xue Xue Bao ; 37(12): 1683-1687, 2017 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-29292266

RESUMO

OBJECTIVE: We report a case of in vitro fertilization and embryo transfer (IVF?ET) with oocyte donation in a woman with premature ovarian insufficiency (POI) complicated by systemic lupus erythematosus (SLE) during pregnancy. The patient had a diagnosis of POI 4 years earlier and 11 weeks after successful pregnancy by IVF?ET with oocyte donation in 2003, she presented with facial edema, and further examinations confirmed the diagnosis of lupus nephritis. She received treatment with prednisone to control the activity of SLE and aspirin and low?molecular?weight heparin to improve placental blood flow with close monitoring of gravida and fetus throughout pregnancy. The condition of the patient remained unstable during pregnancy, and liver damage and placental circulation disorder occurred in late gestational weeks with suspected intrauterine growth retardation (IUGR) of the fetus. For maternal and fetal safety, the patient received elective caesarean section and delivered a premature boy at 31 weeks of gestation. She subsequently received further medications for SLE and showed good recovery of the immunological parameters and absence of SLE symptoms during the follow?up for 14 years, indicating a clinical cure of SLE. Her son shows normal growth and development. Based on the experience with this case and literature review, we believe that immunological factor is an important cause of POI and thus recommend full immunological examinations in cases of idiopathic POI.


Assuntos
Lúpus Eritematoso Sistêmico/complicações , Resultado da Gravidez , Insuficiência Ovariana Primária/complicações , Aspirina/uso terapêutico , Cesárea , Transferência Embrionária , Feminino , Fertilização in vitro , Humanos , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Nefrite Lúpica/complicações , Prednisona/uso terapêutico , Gravidez , Complicações na Gravidez
8.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(12): 1632-1637, 2016 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-27998857

RESUMO

OBJECTIVE: To investigate the clinical outcomes in vitro fertilization or intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) in women aged over 40 years. METHODS: We retrospectively analyzed 1050 non-donor IVF/ICSI-ET cycles performed from January, 2007 to December, 2015 in women at the age 40 years or above, including 393 women at 40 years of age, 266 at 41 years, 158 at 42 years, 107 at 43 years, 64 at 44 years, and 65 at 45-51 years. The clinical characteristics and outcomes of the women in different age groups were compared and analyzed. The pregnancy outcome of different ovarian stimulation protocols and different numbers of embryo transferred were also compared. RESULTS: Oocyte retrieval was achieved in 1032 treatment cycles. Of the 750 embryo transfer cycles, the clinical pregnancy rate was 17.7% (113/750), and the live birth rate was 8.5% (64/750). The clinical pregnancy rate in the 5 age groups was 23.4%, 21.0%, 13.1%, 9.2%, 5.6% and 0%, and the implantation rate was 11.2%, 10.2%, 6.3%, 5.1%, 2.3% and 0%, respectively; the early spontaneous abortion rate was 31.0%, 35.9%, 42.9%, 42.9% and 100%, and the live birth rate was 11.9%, 11.8%, 2.8% and 3.9%. The clinical pregnancy rates of long protocol, short prorocol, GnRHa antagonist protocol, and ovulation induction protocol were 23.6%, 10.2%, 13.3%, and 2.3%, respectively. In the 750 transfer cycles, the clinical pregnancy rate was 3.8% with single embryo transfer, 12.6% with double embryos transfer, and 23.0% with 3 embryos transfer. CONCLUSION: In women aged 40 years or above, the clinical pregnancy rate decreased significantly with age, and the live birth rate was extremely low in women aged beyond 44 years. Assisted reproductive technique is recommended for women aged 40 years and above even when no identifiable causes of sterility are present. For women aged above 44 years of age, oocyte donation may be a better option.


Assuntos
Transferência Embrionária , Transferência de Embrião Único , Injeções de Esperma Intracitoplásmicas , Aborto Espontâneo , Adulto , Implantação do Embrião , Feminino , Fertilização in vitro , Humanos , Infertilidade , Pessoa de Meia-Idade , Doação de Oócitos , Recuperação de Oócitos , Indução da Ovulação , Gravidez , Resultado da Gravidez , Taxa de Gravidez , Estudos Retrospectivos
9.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(11): 1477-1481, 2016 Nov 20.
Artigo em Chinês | MEDLINE | ID: mdl-27881336

RESUMO

OBJECTIVE: To analyze the incidence, management, and outcomes of monozygotic twin (MZT) pregnancy conceived by assisted reproductive techniques (ART). METHODS: A retrospective analysis was performed of clinical pregnancies after in vitro fertilization and embryo transfer (IVF-ET) and introcytoplasmic sperm injection and embryo transfer (ICSI-ET) from January, 2010 to June 2015 at our center. We investigated the incidence, managements and outcomes of 94 MZT pregnancies. Comparison of the pregnancy outcomes was made between the expectantly managed MZT pregnancies, dizygotic twin (DZT) pregnancies, monozygotic (MZ)-triplet pregnancies with selective embryo reduction (SER) to 2 fetuses and 1 fetus, and non-MZ triplet pregnancies with SER to 2 fetuses. RESULTS: Ninety-four MZT pregnancies occurred in the total of 6257 clinical pregnancy cycles with an incidence of 1.5%. No significant difference was found in the incidence of MZT pregnancies between IVF and ICSI cycles or between fresh and thawed cycles (P>0.05). Of the 94 MZT pregnancies, 45 were MZT pregnancy cycles, 43 were MZ-triplet pregnancy cycles, 3 were MZ-quadruplet pregnancy cycles and 3 were ectopic pregnancies. The expectantly managed MZT was associated with a significantly greater rate of miscarriage and malformation and a lower rate of live birth and term birth (P<0.05) in comparison with DZT pregnancy cycles that did not undergo SER. Similar outcomes were found between MZ-triplet pregnancies with SER to 2 fetuses and MZ-triplet pregnancies with SER to 1 fetus (P>0.05), and between MZ-triplets with SER to 2 fetuses and non-MZ triplet pregnancies with SER to 2 fetuses (P>0.05). CONCLUSION: ART is associated with a much higher incidence of MZT pregnancies than spontaneous conception. MZT pregnancies are at high risk of adverse outcomes, and reduction of MZT in multiple pregnancies may help to improve the outcomes.


Assuntos
Resultado da Gravidez , Gravidez de Gêmeos , Técnicas de Reprodução Assistida , Gêmeos Monozigóticos , Transferência Embrionária , Feminino , Fertilização in vitro , Humanos , Incidência , Nascido Vivo , Gravidez , Estudos Retrospectivos , Injeções de Esperma Intracitoplásmicas
10.
Medicine (Baltimore) ; 95(43): e5199, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27787375

RESUMO

The aim of our study was to compare the clinical characteristics of fetal and neonatal outcomes in twin pregnancies between women with preeclampsia (PE) and those with normotension in a Chinese population.There were 143 preeclamptic women and 367 normotensive women with twin pregnancies included in this retrospective case-control study. The baseline characteristics and perinatal outcomes were collected and compared between the groups. Multiple logistic regression and linear regression were used to assess the correlations between PE and the outcomes.Significant increases were observed in the frequencies of preterm delivery (OR = 2.75, P < 0.001), iatrogenic preterm birth (OR = 3.52, P < 0.001), and IUGR (OR = 2.94, P = 0.001) in the PE group, and the PE group had more than a 2-fold risk of adverse neonatal outcomes. Preeclamptic twin neonates had lower birth weights (ß = -147.34, P = 0.005; ß = -169.47, P = 0.001). The comparison on the discordance of intertwin weight was not significantly different.Twin pregnancies with PE are associated with worse perinatal outcomes. The adverse outcomes of preeclamptic twin pregnancies may be associated with lower birth weights rather than the discordance of the intertwin weight, which requires further confirmation. The results may provide helpful references for better clinical assessments, evaluations of prognosis, and a deeper understanding of preeclamptic twin pregnancies.


Assuntos
Feto/diagnóstico por imagem , Pré-Eclâmpsia/diagnóstico , Resultado da Gravidez/epidemiologia , Gravidez de Gêmeos , Nascimento Prematuro/epidemiologia , Adolescente , Adulto , China/epidemiologia , Feminino , Humanos , Recém-Nascido , Masculino , Pré-Eclâmpsia/epidemiologia , Gravidez , Estudos Retrospectivos , Ultrassonografia Pré-Natal , Adulto Jovem
11.
Zhongguo Zhong Yao Za Zhi ; 41(9): 1608-1616, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-28891607

RESUMO

In recent years, with the price rise of Amomum villosum, the quality of A. villosum in the market has been in disorder. To understand the quality status of A. villosum in the market and provide reference for the commercial size fifty-seven samples were collected from different producing areas or markets from August 2013. The samples were detected with evaluation on appearance quality, determination of the contents of bornyl acetate, determination of pesticide residues and heavy metals residues based on Chinese Pharmacopoeia 2015. The results showed that the pesticide residues and heavy metals residues met the requirments, all the samples from different producing areas were qualified except one sample from Fujian province. The qualified rate of native products and imports products samples from market were 43.75% and 14.29%, respectively, the qualified rate of the samples of Yunnan province from producing areas was higher than that from the market. There are two ports at the national level in Yunnan province, where the southern herbs from. A. villosumis one of import medicines from Southeast Asia, and lots of A. villosum samples import to China from Yunnan ports. Most of pharmacists believed that all of the samples from Yunnan province produced in Yunnan. The great majority of commercial species was A. villosum, but A. longiliglare was scarce. Through the survey, it isfound that the main factors affecting the quality of Amomi Fructus was source, lots of A. villosum samples have been replaced by the Amomi Fructus, so the source of imports Amomi Fructus was not clear, which was also more difficult to identify. The quality of A. villosum needs to protect, optimize germplasm, strict control of medicinal sources, specification for medicinal harvesting and processing technology.


Assuntos
Amomum , Medicamentos de Ervas Chinesas/normas , China , Frutas , Controle de Qualidade
12.
PLoS One ; 9(5): e97775, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24830795

RESUMO

OBJECTIVE: Quantified risks of congenital Toxoplasma gondii infection and abnormal pregnancy outcomes following primary maternal infection were evaluated with meta- analysis based on published studies. METHODS: The related literatures were searched in multiple literature databases regardless of languages. Odds ratio (OR) and 95% confidence interval (CI) were used to evaluate the risks of vertical transmission of Toxoplasma gondii and abnormal pregnancy outcomes following primary maternal infection with meta-analysis. RESULTS: 53 of the 2632 searched literatures were included in our analysis. The incidence of abnormal pregnancy outcomes in T. gondii infected pregnant women (infected group) was significantly higher than that in the uninfected pregnant women (control group) (OR = 5.10; 95% CI, 3.85-6.75). Toxoplasma gondii infection rate in the abnormal-pregnancy-outcome group was significantly higher than in the normal-pregnancy group (OR = 3.71; 95% CI, 3.31-4.15). The pooled rate of vertical transmission was 20% (95% CI, 15%-26%) in maternal infection of T. gondii. The incidences of vertical transmission in women who were infected in the first, second or third trimester of pregnancy were 5% (95%CI, 2%-16%), 13% (95%CI, 7%-23%), and 32% (95%CI, 24%-41%), respectively. The rates of vertical transmission in women who were treated with spiramycin-only, PSF (pyrimethamine + sulfadiazine + folinic acid) or PS (pyrimethamine + sulfadiazine) combined with spiramycin, or other untypical treatments were 13% (95%CI, 7%-22%), 13%(95%CI, 7%-25%), and 24%(95%CI, 18%-32%), respectively. CONCLUSIONS: Toxoplasma gondii infection can result in adverse pregnancy outcomes in pregnant women. The pooled rate of vertical transmission was 20% in maternal infection and the incidences of vertical transmission increased in the first, second or third trimester of pregnancy. The pooled rates of transmission in groups treated with spiramycin-only, PSF or PS combined with spiramycin, or other untypical treatments were not significantly different.


Assuntos
Complicações Parasitárias na Gravidez/etiologia , Toxoplasmose/transmissão , Feminino , Humanos , Incidência , Transmissão Vertical de Doenças Infecciosas , Gravidez , Complicações Parasitárias na Gravidez/tratamento farmacológico , Complicações Parasitárias na Gravidez/epidemiologia , Resultado da Gravidez , Risco , Toxoplasmose/tratamento farmacológico , Toxoplasmose/epidemiologia
13.
Zhong Yao Cai ; 36(1): 122-7, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23750419

RESUMO

OBJECTIVE: To obtain the optimal conditions of extraction of Clerodendranthus spicatus and provide the theoretical foundation for its further processing and utilization. METHODS: On the basis of the single factor test, response surface methodology was applied to optimize the extraction conditions. RESULTS: The results showed that extraction time, water-feed ratio, ethanol concentration and extraction temperature all had significant effects on the extraction rate of polysaccharides. The optimal extraction time was 3h, solid-liquid ratio was 50:1, ethanol concentration was 30% and extraction temperature was 80 degrees C. Under these optimized conditions, the extraction rate was 27.71%. CONCLUSION: The extraction technology is simple, reliable and highly predictive.


Assuntos
Cinamatos/análise , Depsídeos/análise , Medicamentos de Ervas Chinesas/isolamento & purificação , Lamiaceae/química , Tecnologia Farmacêutica/métodos , Cromatografia Líquida de Alta Pressão , Cinamatos/isolamento & purificação , Depsídeos/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Etanol/química , Temperatura , Fatores de Tempo , Ácido Rosmarínico
14.
Diabetes Care ; 36(3): 586-90, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23193214

RESUMO

OBJECTIVE: To evaluate the value of fasting plasma glucose (FPG) value in the first prenatal visit to diagnose gestational diabetes mellitus (GDM). RESEARCH DESIGN AND METHODS: Medical records of 17,186 pregnant women attending prenatal clinics in 13 hospitals in China, including the Peking University First Hospital (PUFH), were examined. Patients with pre-GDM were excluded; data for FPG at the first prenatal visit and one-step GDM screening with 75-g oral glucose tolerance test (OGTT) performed between 24 and 28 weeks of gestation were collected and analyzed. RESULTS: The median ± SD FPG value was 4.58 ± 0.437. FPG decreased with increasing gestational age. FPG level at the first prenatal visit was strongly correlated with GDM diagnosed at 24-28 gestational weeks (χ(2) = 959.3, P < 0.001). The incidences of GDM were 37.0, 52.7, and 66.2%, respectively, for women with FPG at the first prenatal visit between 5.10 and 5.59, 5.60 and 6.09, and 6.10-6.99 mmol/L. The data of PUFH were not statistically different from other hospitals. CONCLUSIONS: Pregnant women (6.10 ≤ FPG < 7.00 mmol/L) should be considered and treated as GDM to improve outcomes; for women with FPG between 5.10 and 6.09 mmol/L, nutrition and exercise advice should be provided. An OGTT should be performed at 24-28 weeks to confirm or rule out GDM. Based on our data, we cannot support an FPG value ≥5.10 mmol/L at the first prenatal visit as the criterion for diagnosis of GDM.


Assuntos
Glicemia/metabolismo , Diabetes Gestacional/diagnóstico , Jejum/sangue , China , Feminino , Idade Gestacional , Humanos , Gravidez , Cuidado Pré-Natal
15.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 30(3): 252-4, 2010 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-20535920

RESUMO

OBJECTIVE: To evaluate the efficacy of Salviae injection and ligustrazine injection (S & L) in combining with Western medicine for treatment of early onset of severe pre-eclampsia (EOSP), and its impact on pregnancy. METHODS: An open prospective controlled study was conducted on 63 women suffering from EOSP during the 29th-32th week of pregnancy. They were assigned, according to the sequence of hospitalizing, to the treated group (32 cases) and the control group (31 cases). Both groups were treated with conventional Western medical treatment, but S & L were administered additionally to the treated group. Levels of plasma D-dimer, blood viscosity, and outcome of the pregnancy were analyzed. RESULTS: The effect in prolonging the pregnancy and lowing the incidence of fetal distress and newborn death in the treated group was superior to that in the control group, the corresponding data were 11.0 +/- 1.2 days, 11 cases, 4 cases in the treated group and 6.0 +/- 1.5 days, 16 cases and 8 cases in the control group, respectively. Levels of blood viscosity ratio and D-dimer in the treated group were lowered significantly after treatment (1.20 +/- 0.13 vs 2.29 +/- 0.13, 1.61 +/- 0.45 mg/L vs 2.40 +/- 0.09 mg/L), which had no remarkable change in the control group. CONCLUSION: Combined application of S & L for treatment of EOSP could improve the blood hyperviscosity, prolong the pregnancy week, and improve the outcome of pregnancy to reduce incidence of perinatal morbidity and mortality in patients.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Integrativa/métodos , Pré-Eclâmpsia/tratamento farmacológico , Complicações na Gravidez/tratamento farmacológico , Adulto , Feminino , Humanos , Gravidez , Terceiro Trimestre da Gravidez , Estudos Prospectivos , Pirazinas/uso terapêutico , Salvia miltiorrhiza/química , Adulto Jovem
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