Selective and sensitive visible-light-prompt photoelectrochemical sensor of paracetamol based on Bi2WO6 modified with Bi and copper sulfide.

Paracetamol (PA) is a ubiquitous non-steroidal anti-inflammatory drug, mainly used to treat headaches, arthritis and osteoarthritis and other diseases. In this work, a novel label free photoelectrochemical (PEC) sensor based on Bi-CuS/Bi2WO6 has been developed for the detection of PA, which was fabricated by a simple two-step hydrothermal process. It was found that Bi-CuS/Bi2WO6 with a CuS/Bi2WO6 heterojunction and surface plasmon resonance (SPR) effect of Bi possesses enhanced charge transfer and absorption wavelengths under visible light, particularly when compared to pristine Bi2WO6 films, thus producing an increase in the observed photocurrent. The photocurrent was increased after adding PA. And the photocurrent increment was linear with PA concentration in the range from 0.01-60 µM with a detection limit of 2.12 nM. Moreover, the PEC sensor also exhibited high anti-interference property and acceptable stability. In the present study, a Bi-CuS/Bi2WO6 photoelectrode is considered a promising candidate for carrying out PEC analysis.

Target therapy of TRIM-14 inhibits osteosarcoma aggressiveness through the nuclear factor-κB signaling pathway.

Osteosarcoma is the most common cause of cancer-associated mortality and the prognosis is yet to be fully elucidated due to the paucity of effective therapeutic targets that significantly influence the quality of life and mean survival rates of patients with osteosarcoma. Studies have showed that tripartite motif-containing (TRIM)-14 is a member of the TRIM protein family that has a vital role in tumor progression and metastasis and promotes angiogenesis, invasion and apoptotic resistance of bone cancer. In this study, a chimeric antibody targeting TRIM-14 (Chanti-TRIM) was constructed and the molecular mechanism of target therapy for TRIM-14 was investigated in osteosarcoma cells and xenograft mice. The growth, migration and invasion properties of U-2OS cells were analyzed following incubation with 10-160 mg/ml Chanti-TRIM. Apoptosis of U-2OS cells was detected after Chanti-TRIM treatment. Matrix metalloproteinase (MMP)-9-mediated nuclear factor-κB (NF-κB) signal pathway was analyzed in U-2OS cells treated with Chanti-TRIM. The inhibitory efficacy of Chanti-TRIM was studied in U-2OS-bearing xenograft mice. Our results demonstrated that neutralizing TRIM-14 expression markedly inhibited the growth, migration and invasion of osteosarcoma cells, in vitro and in vivo. We found that TRIM-14 depletion decreased cell viability and induced cells apoptosis in vitro. In addition, we identified Chanti-TRIM inhibited growth and promoted apoptosis induced by cisplatin through MMP-9-mediated NF-κB signal pathway. Furthermore, we observed that Chanti-TRIM treatment inhibited osteosarcoma growth in vivo. Histological analysis indicated that apoptotic bodies were increased and NF-κB nuclear translocation factors, including Ikkß, p65 and IkBα, were decreased in tumors treated by Chanti-TRIM. In conclusion, these results showed that Chanti-TRIM markedly inhibited the progression of osteosarcoma, suggesting Chanti-TRIM may be a potential anti-cancer agent that functions via the activation of the NF-κB pathway for osteosarcoma.

[Inhibitory effect of overexpressed kisspeptin on proliferation and migration of MKN-45 gastric cancer cells].

Objective: To investigate the expression of kisspeptin in gastric adenocarcinoma and its effect on the proliferation and migration of gastric cancer cells. Methods: The level of kisspeptin in 50 gastric cancer tissues and their paracancerous tissues were detected by quantitative real-time PCR and Western blotting. Kisspeptin-siRNA,control-siRNA,p EGFP-N1-kisspeptin,p EGFP-N1 were separately transfected into MKN-45 gastric cancer cells. After 48-hour culture,the levels of matrix metalloproteinase-2( MMP-2),ß-catenin,C-myc,MMP-9,kisspeptin were detected by Western blotting; MTT assay was used to detect the proliferation of MKN-45 cells; wound healing assay was performed to assess the migration ability of MKN-45 cells. After MKN-45 cells were treated with Wnt / ß-catenin signal pathway inhibitor FH535,MTT assay and flow cytometry were used to evaluate cell proliferation ability and cell apoptosis,respectively. Results: Kisspeptin expression in gastric adenocarcinoma tissues was significantly lower than that of the adjacent normal tissues. The cell survival rate and migration rate of p EGFP-N1-kisspeptin group were significantly lower than those of p EGFP-N1 group. Cell survival rate and migration rate of kisspeptin-siRNA group were significantly higher than those of control siRNA group. The levels of MMP-9,MMP-2,ß-catenin and C-myc in p EGFP-N1-kisspeptin group were significantly lower than those in p EGFP-N1 group. The levels of MMP-9, MMP-2, ß-catenin and C-myc in kisspeptin-siRNA group were significantly higher than those in control-siRNA group. The proliferation and migration trend of gastric cancer cells treated with FH535 was similar to that of the p EGFP-N1-kisspeptin group. Conclusion: The expression of kisspeptin decreases in gastric cancer tissues,and kisspeptin can interact with Wnt / ß-catenin signaling pathway to inhibit the proliferation and migration of gastric cancer cells.