Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Environ Sci Pollut Res Int ; 31(20): 29525-29535, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38575819

RESUMO

Antibiotics have been widely detected in aquatic environments, and fungal biotransformation receives considerable attention for antibiotic bioremediation. Here, a fungus designated Cladosporium cladosporioides 11 (CC11) with effective capacity to biotransform fluoroquinolones was isolated from aquaculture pond sediments. Enrofloxacin (ENR), ciprofloxacin (CIP) and ofloxacin (OFL) were considerably abated by CC11, and the antibacterial activities of the fluoroquinolones reduced significantly after CC11 treatment. Transcriptome analysis showed the removal of ENR, CIP and OFL by CC11 is a process of enzymatic degradation and biosorption which consists well with ligninolytic enzyme activities and sorption experiments under the same conditions. Additionally, CC11 significantly removed ENR in zebrafish culture water and reduced the residue of ENR in zebrafish. All these results evidenced the potential of CC11 as a novel environmentally friendly process for the removal of fluoroquinolones from aqueous systems and reduce fluoroquinolone residues in aquatic organisms.


Assuntos
Biodegradação Ambiental , Cladosporium , Fluoroquinolonas , Poluentes Químicos da Água , Cladosporium/metabolismo , Fluoroquinolonas/farmacologia , Fluoroquinolonas/metabolismo , Poluentes Químicos da Água/metabolismo , Aquicultura , Antibacterianos/farmacologia , Sedimentos Geológicos/microbiologia , Animais , Peixe-Zebra
2.
Environ Sci Technol ; 58(2): 1022-1035, 2024 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-38165294

RESUMO

There is epidemiological evidence in humans that exposure to endocrine-disrupting chemicals such as bisphenol A (BPA) is tied to abnormal neuroendocrine function with both behavioral and intestinal symptoms. However, the underlying mechanism of this effect, particularly the role of gut-brain regulation, is poorly understood. We exposed zebrafish embryos to a concentration series (including environmentally relevant levels) of BPA and its analogues. The analogue bisphenol G (BPG) yielded the strongest behavioral impact on zebrafish larvae and inhibited the largest number of neurotransmitters, with an effective concentration of 0.5 µg/L, followed by bisphenol AF (BPAF) and BPA. In neurod1:EGFP transgenic zebrafish, BPG and BPAF inhibited the distribution of enteroendocrine cells (EECs), which is associated with decreased neurotransmitters level and behavioral activity. Immune staining of ace-α-tubulin suggested that BPAF inhibited vagal neural development at 50 and 500 µg/L. Single-cell RNA-Seq demonstrated that BPG disrupted the neuroendocrine system by inducing inflammatory responses in intestinal epithelial cells via TNFα-trypsin-EEC signaling. BPAF exposure activated apoptosis and inhibited neural developmental pathways in vagal neurons, consistent with immunofluorescence imaging studies. These findings show that both BPG and BPAF affect the neuroendocrine system through the gut-brain axis but by different mechanisms, revealing new insights into the modes of bisphenol-mediated neuroendocrine disruption.


Assuntos
Sistemas Neurossecretores , Fenóis , Peixe-Zebra , Animais , Humanos , Compostos Benzidrílicos/toxicidade , Encéfalo , Neurotransmissores/metabolismo
3.
Eco Environ Health ; 2(2): 79-87, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38075292

RESUMO

Water pollution seriously threatens the sustainable development of fisheries in China. To inform effective pollution control policies, a comprehensive understanding of the fishery environment status is needed. However, nationwide data on the temporal changes of major pollutants in the fishery waters of China are scarce. This study collected data on the major water pollutants, including total nitrogen, total phosphorus, heavy metals, and total petroleum hydrocarbons (TPHs), from 2003 to 2017 to evaluate dynamic changes in the inland fishery water environment across China. We discovered that the levels of four heavy metals (Cu, Zn, Pb, and Cd) and TPH decreased during the 15-year period, corresponding to the reduced national discharge of pollution sources from 2003 to 2015. However, nitrogen and phosphorus levels in the inland fishery waters showed no significant changes during this period. A comparative analysis of water quality in different periods indicated that these improvements were highly associated with effective measures for water pollution control in China. In addition, the decline in pollution was consistent among the three regions of China (north, west, and southeast) from 2003 to 2017, while southeast China exhibited the weakest pollution mitigation among the three regions. These findings suggest that the inland fishery water quality improved during 2003-2017, but still faced eutrophication risk.

4.
Sci Total Environ ; 851(Pt 1): 157974, 2022 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-35963407

RESUMO

The unease of consumers with bisphenol A has led to the increased industrial usage of bisphenol F (BPF), which is a new hazard to environmental health. Here, zebrafish were exposed to three BPF concentrations (0.5, 5, and 50 µg/L) from the embryonic stage for 180 days. Results showed that zebrafish body length and weight decreased and hepatosomatic index values increased, even at environmentally relevant concentration. Histological analysis identified the occurrence of hepatic fibrosis and steatosis in 5 and 50 µg/L groups, which indicated the liver injury caused by BPF. Based on the untargeted metabolomics results, a dose-dependent variation in the effects of BPF on liver metabolism was found, and amino acids, purines and one carbon metabolism were the main affected processes in the 0.5, 5, and 50 µg/L treatments, respectively. At the same time, BPF induced a shift in intestinal microbiome composition, including decreased abundance of Erysipelotrichaceae, Rhodobacteraceae and Gemmobacter. In addition, the correlation analysis suggested an association between gut microbiome changes and affected hepatic metabolites after BPF exposure. These findings indicate that a liver-gut alteration is induced by long-term BPF exposure.


Assuntos
Compostos Benzidrílicos , Peixe-Zebra , Animais , Aminoácidos/metabolismo , Compostos Benzidrílicos/metabolismo , Compostos Benzidrílicos/toxicidade , Carbono/metabolismo , Fígado , Fenóis , Purinas/metabolismo , Intestinos
5.
Environ Sci Pollut Res Int ; 29(58): 87402-87412, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35804233

RESUMO

To comprehensively understand the toxic risks of phthalates to aquatic ecosystems, we examined the acute toxicity of di-(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) on multiple trophic models, including algae (Chlorella vulgaris), Daphnia magna and fish (Danio rerio, Pseudorasbora parva). Thus, a 15-day zebrafish exposure was conducted to trace the dynamic changes of phthalate-induced toxic effects. Among the four species, D. magna exhibited the strongest sensitivity to both DEHP and DBP, followed by D. rerio and P. parva. C. vulgaris exhibited the lowest sensitivity to phthalates. The sub-chronic zebrafish assay demonstrated that 1000 µg/L DBP induced significant mortality at 15 days post-exposure (dpe), and DEHP exhibited no lethality at the tested concentrations (10-5000 µg/L). Zebrafish hepatic SOD activity and sod transcription levels were inhibited by DBP from 3 dpe, which was accompanied by increased malondialdehyde level, while zebrafish exposed to DEHP exhibited less oxidative damage. Both DEHP and DBP induced time-dependent alterations on Ache activity in zebrafish brains, thus indicating the potential neurotoxicity toward aquatic organisms. Additionally, 1000 µg/L and higher concentration of DBP caused hepatic DNA damage in zebrafish from 7 dpe. These results provide a better understanding of the health risks of phthalate to water environment.


Assuntos
Chlorella vulgaris , Dietilexilftalato , Ácidos Ftálicos , Animais , Dibutilftalato/toxicidade , Dietilexilftalato/toxicidade , Peixe-Zebra , Ecossistema , Ácidos Ftálicos/toxicidade , Superóxido Dismutase
6.
Environ Sci Technol ; 56(12): 8528-8540, 2022 06 21.
Artigo em Inglês | MEDLINE | ID: mdl-35616434

RESUMO

The central nervous system (CNS) is a sensitive target for endocrine-disrupting chemicals, such as bisphenol analogues. Bisphenol A (BPA) usage is associated with the occurrence of many neurological diseases. With the restricted use of BPA, bisphenol F (BPF) has been greatly introduced for industrial manufacture and brings new hazards to public CNS health. To understand how BPF affects the neural system, we performed a cognitive test for zebrafish that are continuously exposed to environmentally relevant concentrations (0.5 and 5.0 µg/L) of BPF since embryonic stage and identified suppressed cognitive ability in adulthood. Single-cell RNA sequencing of neural cells revealed a cell composition shift in zebrafish brain post BPF exposure, including increase in microglia and decrease in neurons; these changes were further validated by immune staining. At the same time, a significant inflammatory response and increased phagocytic activity were detected in zebrafish brain post BPF exposure, which were consistent with the activation of microglia. Cell-specific transcriptomic profiles showed that abnormal phagocytosis, activated brain cell death, and apoptosis occurred in microglia post BPF exposure, which are responsible for the neuron loss. In addition, certain neurological diseases were affected by BPF in both excitatory and inhibitory neurons, such as the movement disorder and neural muscular disease, however, with distinctly involved genes. These findings indicate that BPF exposure could lead to an abnormal cognitive behavior of zebrafish through inducing heterogeneous changes of neural cells in brain and revealed the dominating role of microglia in mediating this effect.


Assuntos
Disruptores Endócrinos , Peixe-Zebra , Animais , Compostos Benzidrílicos/metabolismo , Compostos Benzidrílicos/toxicidade , Cognição , Disruptores Endócrinos/metabolismo , Disruptores Endócrinos/toxicidade , Neurônios , Fenóis , Peixe-Zebra/metabolismo
7.
Environ Int ; 165: 107287, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35598417

RESUMO

The metabolic effects of endocrine-disrupting chemicals, such as bisphenol analogues, have drawn increasing attention. Bisphenol A (BPA) usage is associated with the occurrence of many metabolic diseases. With the restricted use of BPA, alternatives like bisphenol F (BPF) and bisphenol AF (BPAF) have been greatly introduced for industrial manufacture, and brings new hazard to public health. To understand how bisphenol analogues induced metabolic effects, zebrafish are continuous exposed to environmental level (0.5 µg/L) of BPA, BPF and BPAF since embryonic stage, and identified hepatic steatosis and insulin resistance at 60-day post fertilization. Hepatic transcriptional profile indicated that pancreatic disease pathways were activated by BPA, but were inhibited by BPF. At the same time, increased lipid secretion and gluconeogenesis pathways in zebrafish liver was found post BPAF exposure. Significant inflammatory response, histological injury and increased mucus secretion was detected in zebrafish intestine post exposure of three bisphenol analogues. Single-cell RNA sequencing of zebrafish intestinal cells revealed activation of lipid uptake and absorption pathways in enterocyte lineages, which well explained the hepatic steatosis induced by BPA and BPF. Besides, genes related to carbohydrate metabolism, diabetes and insulin resistance were activated in intestinal immune cell types by three bisphenol analogues. These findings indicated that BPA and its alternatives could lead to abnormal lipid and carbohydrate metabolism of zebrafish through inducing cell heterogeneous changes in gut, and revealed both molecular and cellular mechanism in mediating this effect.


Assuntos
Resistência à Insulina , Peixe-Zebra , Animais , Compostos Benzidrílicos/química , Compostos Benzidrílicos/toxicidade , Intestinos , Lipídeos , Fenóis
8.
Sci Total Environ ; 806(Pt 1): 149992, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34844315

RESUMO

Bisphenol F (BPF), as an important bisphenol A substitute, is being increasingly used for industrial production. Here we performed large scale fecundity test for zebrafish that are continuous exposed to environmental levels of BPF (0.5, 5 and 50 µg/L) from embryonic stage, and identified suppressed spawning capacity of females and reduced fertility rate of males in adulthood. Although pathological change is only observed in female gonads, the transcriptional change in the hypothalamic-pituitary-gonad axis genes occurred in the gonads of both female and male fish at 150 days post-exposure. F1 generation embryos showed abnormal developmental outcomes including decreased heart rate, reduced body length, and inhibition of spontaneous movement after parental exposure to BPF. RNA-sequencing showed that the genes involved in skeletal/cardiac muscle development were significantly altered in F1 embryos spawned by BPF-treated zebrafish. The advanced pathway analysis showed that cancer and tumour formation were the most enriched pathways in the offspring of 0.5 and 5.0 µg/L groups; organismal development and cardiovascular system development were mainly affected after parental exposure to 50 µg/L of BPF; these changes were mediated by several involved regulators such as GATA4, MYF6, and MEF2C. These findings confirmed that long-term exposure to BPF at environment relevant concentration would result in reproductive toxicity among zebrafish indicating the urgent demand for the control of BPA substitutes.


Assuntos
Poluentes Químicos da Água , Peixe-Zebra , Animais , Compostos Benzidrílicos/toxicidade , Feminino , Gônadas , Masculino , Fenóis , Reprodução , Poluentes Químicos da Água/toxicidade
9.
Microorganisms ; 9(12)2021 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-34946147

RESUMO

Lactic acid bacteria (LAB) are commonly applied to fish as a means of growth promotion and disease prevention. However, evidence regarding whether LAB colonize the gastrointestinal (GI) tract of fish remains sparse and controversial. Here, we investigated whether Lacticaseibacillus casei ATCC 393 (Lc) can colonize the GI tract of crucian carp. Sterile feed irradiated with 60Co was used to eliminate the influence of microbes, and 100% rearing water was renewed at 5-day intervals to reduce the fecal-oral circulation of microbes. The experiment lasted 47 days and was divided into three stages: the baseline period (21 days), the administration period (7 days: day -6 to 0) and the post-administration period (day 1 to 19). Control groups were fed a sterile basal diet during the whole experimental period, whereas treatment groups were fed with a mixed diet containing Lc (1 × 107 cfu/g) and spore of Geobacillus stearothermophilus (Gs, 1 × 107 cfu/g) during the administration period and a sterile basal diet during the baseline and post-administration periods. An improved and highly sensitive selective culture method (SCM) was employed in combination with a transit marker (a Gs spore) to monitor the elimination of Lc in the GI tract. The results showed that Lc (<2 cfu/gastrointestine) could not be detected in any of the fish sampled from the treatment group 7 days after the cessation of the mixed diet, whereas Gs could still be detected in seven out of nine fish at day 11 and could not be detected at all at day 15. Therefore, the elimination speed of Lc was faster than that of the transit marker. Furthermore, high-throughput sequencing analysis combined with SCM was used to reconfirm the elimination kinetics of Lc in the GI tract. The results show that the Lc in the crucian carp GI tract, despite being retained at low relative abundance from day 7 (0.11% ± 0.03%) to 21, was not viable. The experiments indicate that Lc ATCC 393 cannot colonize the GI tract of crucian carp, and the improved selective culture in combination with a transit marker represents a good method for studying LAB colonization of fish.

10.
Environ Pollut ; 265(Pt B): 113876, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32806432

RESUMO

The potential risks of phthalates affecting human and animal health as well as the environment are emerging as serious concerns worldwide. However, the mechanism by which phthalates induce developmental effects is under debate. Herein, we found that embryonic exposure of zebrafish to di-(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) increased the rate of heart defects including abnormal heart rate and pericardial edema. Changes in the transcriptional profile demonstrated that genes involved in the development of the heart, such as tbx5b, nppa, ctnt, my17, cmlc1, were significantly altered by DEHP and DBP at 50 µg/L, which agreed with the abnormal cardiac outcomes. Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) further showed that significant hypomethylation of nppa and ctnt was identified after DEHP and DBP exposure, which was consistent with the up-regulation of these genes. Notably, hypermethylation on the promoter region (<1 kb) of tbx5b was found after DEHP and DBP exposure, which might be responsible for its decrease in transcription. In conclusion, phthalates have the potential to induce cardiac birth defects, which might be associated with the transcriptional regulation of the involved developmental factors such as tbx5b. These findings would contribute to understand the molecular pathways that mediated the cardiac defects caused by phthalates.


Assuntos
Ácidos Ftálicos , Peixe-Zebra , Animais , Dibutilftalato , Coração , Humanos
11.
Chemosphere ; 249: 126144, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32086060

RESUMO

Phthalates (phthalate esters, PAEs) are commonly used as plasticizers and are emerging concerns worldwide for their potential influence on the environment and general public health. Thus, identification of the negative effects and involved mechanisms of PAEs is necessary. Herein, we found that embryonic exposure of zebrafish to di-(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) significantly induced spinal defects, such as inhibited spontaneous movement at 24 h post-fertilization (hpf), spine curvature and body length decrease at 96 hpf. The transcriptional level of the genes that are related to the development of the notochord (col8a1a and ngs), muscle (stac3, klhl41a and smyd2b) and skeleton (bmp2, spp1) were significantly altered by DEHP and DBP at 50 and 250 µg/L, which might be associated with the observed morphological changes. Notably, DBP and DEHP altered the locomotor activity of zebrafish larvae at 144 hpf, which might be due to the abnormal development of the spine and skeletal system. In conclusion, phthalates caused spinal birth defects in zebrafish embryos, induced transcriptional alterations of the spinal developmental genes, and led to abnormal behavior.


Assuntos
Bioensaio , Ácidos Ftálicos/toxicidade , Coluna Vertebral/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/embriologia , Proteínas Adaptadoras de Transdução de Sinal , Animais , Dibutilftalato , Dietilexilftalato , Embrião não Mamífero/efeitos dos fármacos , Larva , Plastificantes , Proteínas de Peixe-Zebra
12.
Environ Sci Technol ; 53(24): 14638-14648, 2019 12 17.
Artigo em Inglês | MEDLINE | ID: mdl-31702913

RESUMO

In this study, the influence of bisphenol F (BPF) toward central nervous system (CNS) was assessed using zebrafish embryos. We found that BPF could induce significant neurotoxicity toward zebrafish embryos, including inhibited locomotion, reduced moving distance, and CNS cell apoptosis at an effective concentration of 0.0005 mg/L. Immunofluorescence assay showed that both microglia and astrocyte in zebrafish brain were significantly activated by BPF, indicating the existence of neuroinflammatory response. Peripheral motor neuron development was significantly inhibited by BPF at 72 hpf. RNA-seq data indicated that neuronal developmental processes and cell apoptosis pathways were significantly affected by BPF exposure, which was consistent with the phenotypic results. Chip-seq assay implied that the transcriptional changes were not mediated by ERα. Additionally, no significant change was found in neurotransmitter levels (5-hydroxytryptamine, dopamine, and acetylcholine) or acetylcholinesterase (Ache) enzyme activity after BPF exposure, indicating that BPF may not affect neurotransmission. In conclusion, BPF could lead to abnormal neural outcomes during zebrafish early life stage through inducing neuroinflammation and CNS cell apoptosis even at environmentally relevant concentration.


Assuntos
Compostos Benzidrílicos , Peixe-Zebra , Animais , Bioensaio , Embrião não Mamífero , Fenóis
13.
Sci Total Environ ; 687: 877-884, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31412491

RESUMO

With the increasing use of bisphenol F (BPF) as BPA alternative, BPF are widely distributed in multiple environment media. Our previous study demonstrated that BPF possess equivalent toxicity towards zebrafish as BPA, while its toxic mechanism remains largely unknown. To investigate the mechanisms mediating the developmental effects of BPF, zebrafish embryos were exposed to 0.0005, 0.5, and 5.0 mg/L BPF. Morphological examination indicated that BPF exposure led to depigmentation, decreased heart rate, inhibited spontaneous movement, hatch inhibition, and spinal deformation. Motor neuron-green fluorescence zebrafish assay indicated that exposure to 0.5 or 5.0 mg/L BPF affected embryonic motor neuron development, which is consistent with the spinal defect and spontaneous movement inhibition. Transcriptomic analysis showed that genes associated with the observed symptoms, including neuron development (ngln2a, socs3a, fosb), cardiac development (klf2a), and spinal deformation (ngs, col8a1a, egr2a), were down-regulated after exposure to either 0.0005 (environmental relevant concentration) or 0.5 mg/L BPF. This partially explained the mechanisms underlying the effects of BPF. In conclusion, BPF had the potential to affect zebrafish development even at environmental level through down-regulating associated genes.


Assuntos
Compostos Benzidrílicos/toxicidade , Desenvolvimento Embrionário/efeitos dos fármacos , Fenóis/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/embriologia , Animais , Larva , Peixe-Zebra/fisiologia
14.
Environ Pollut ; 242(Pt A): 952-961, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30373040

RESUMO

To investigate the developmental effects and corresponding molecular mechanism of diesel in freshwater organisms, zebrafish embryos were exposed to 0# diesel water-accommodated fraction (WAF) at different concentrations. Mortality, embryonic morphological endpoints, transcriptional profile and lipid profile were evaluated after exposure. Exposure to 0# diesel WAF had no significant effect on the survival of zebrafish embryos from 1.5 to 96 hpf. However, a significant increase in mortality was observed at 144 and 196 hpf in the groups of 20 and 40 mg/L 0# diesel WAF. RNA-Seq results demonstrated that 0# diesel WAF could induce significant alterations in transcription profile at concentrations of 0.05 mg/L (the limit for petroleum hydrocarbon concentration in surface water in China) and 5 mg/L. Gene Ontology enrichment and similarity analysis indicated that lipid metabolism, lipid synthesis, biological transport, drug metabolism and homeostatic processes were the most altered biological processes after exposure to 0# diesel WAF. Further, transcription levels of genes involved in cholesterol and fatty acid synthesis were significantly inhibited by diesel WAF according to qPCR results. Lipidomics results also indicated that several lipid species (cholesterol ester, fatty acid, diglyceride and triglyceride) decreased after 0# diesel WAF exposure. These results reflect the potential risk of diesel pollution in freshwater ecosystems especially on the alteration of lipid homeostasis and enable a better understanding of the molecular pathways underlying the action of diesel WAF in zebrafish embryos.


Assuntos
Embrião não Mamífero/efeitos dos fármacos , Petróleo/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , China , Ecossistema , Homeostase , Hidrocarbonetos/análise , Metabolismo dos Lipídeos , Lipídeos , Petróleo/análise , Água/análise , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo , Peixe-Zebra/embriologia , Peixe-Zebra/metabolismo , Peixe-Zebra/fisiologia
15.
Environ Pollut ; 241: 674-683, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29902750

RESUMO

To investigate the biological pathways involved in phthalate-induced developmental effects, zebrafish embryos were exposed to different concentrations of di-(2-ethylhexyl) (DEHP) and di-butyl phthalate (DBP) for 96 h. Embryonic exposure to DEHP and DBP induced body length decrease, yolk sac abnormities, and immune responses (up-regulation of immune proteins and genes). The lipidomic results showed that at a concentration of 50 µg/L, DEHP and DBP significantly reduced the levels of fatty acids, triglycerides, diacylglycerol, and cholesterol. These effects are partly explained by biological pathway enrichment based on data from the transcriptional and proteomic profiles. Co-exposure to DBP and ER antagonist did not significantly relieve the toxic symptoms compared with exposure to DBP alone. This indicates that phthalate-induced developmental abnormities in zebrafish might not be mediated by the ER pathway. In conclusion, we identified the possible biological pathways that mediate phthalate-induced developmental effects and found that these effects may not be driven by estrogenic activation.


Assuntos
Ácidos Ftálicos/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/fisiologia , Animais , Dibutilftalato/metabolismo , Dietilexilftalato/toxicidade , Proteômica , Peixe-Zebra/metabolismo
16.
Environ Sci Technol ; 52(5): 3222-3231, 2018 03 06.
Artigo em Inglês | MEDLINE | ID: mdl-29397701

RESUMO

In order to understand the negative effects of bisphenol A (BPA) alternatives comprehensively, zebrafish embryos were used to assess the lethality, developmental effects, and estrogenic activity of bisphenol analogues. The in silico estrogenic activities of bisphenol analogues were assayed by binding simulation. According to our results, the lethality of bisphenol analogues decreased in order of bisphenol AF (BPAF) > BPA > bisphenol F (BPF) > bisphenol S (BPS). BPAF and BPF induced significant effects on zebrafish embryos, including decreased heart rate, hatching inhibition, and teratogenic effects. The binding potentials of bisphenol analogues toward zebrafish ERs (zfERS) decreased in the following order: BPAF > BPA > BPF > BPS. Among the three subtypes of zfERs, zfERß2 showed the highest binding activity toward the bisphenols, followed by zfERα and zfERß1. In vivo estrogenic activity tests showed that BPAF, BPA, and BPF significantly enhanced the protein levels of ERα along with the mRNA levels of esr1, esr2a, esr2b, and vtg1 in zebrafish embryos. Esr2b showed the strongest response to BPAF and BPA exposure among the three esrs. In contrast, BPS did not significantly regulate ER protein level or ER transcription. In conclusion, BPAF showed the highest lethality, developmental effects, and estrogenic activity (both in silico and in vivo) followed by BPA and BPF. BPS showed the weakest toxicity and estrogenic activity. zfERß2 might act as the main target among the three ER subtypes of zebrafish after exposure to BPAF and BPA.


Assuntos
Compostos Benzidrílicos , Peixe-Zebra , Animais , Bioensaio , Estrona , Fenóis
17.
Environ Pollut ; 229: 312-320, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28601763

RESUMO

Although the toxicity of beta-cypermethrin (beta-CYP) to aquatic organisms has become a significant concern in recent years, its enantioselective effects on non-target organisms is poorly understood. To investigate the enantioselective toxicity of beta-CYP on zebrafish, adult zebrafish were exposed to a series of isometric concentrations of four beta-CYP enantiomers and the beta-CYP racemate for 96 h. In addition, the activities of four antioxidant enzymes and the malondialdehyde (MDA) content in zebrafish liver and brain were tested after 15 and 30 days beta-CYP enantiomers and racemate exposure under environmentally relevant dosages (0.01 and 0.1 µg/L). According to the acute toxicity results, the 1R-cis-αS and 1R-trans-αS enantiomers were more lethal than 1S-cis-αR and 1S-trans-αR. At 0.1 µg/L, the 1R-cis-αS and 1R-trans-αS enantiomers, and the beta-CYP racemate could significantly induce a hepatic MDA content at 30 days post exposure (dpe), while only 1R-cis-αS caused brain lipid peroxidation. An apparent regulation of antioxidase levels was observed in zebrafish liver and brain after exposure to the 1R-cis-αS and 1R-trans-αS enantiomers, and the beta-CYP racemate. In contrast, no significant oxidative stress was observed in zebrafish exposed to 1S-cis-αR and 1S-trans-αR enantiomers under the test concentrations. This work demonstrated the occurrence of enantioselectivity in toxicity and oxidative stress of beta-CYP to adult zebrafish, which should be considered in environmental risk assessments.


Assuntos
Inseticidas/toxicidade , Piretrinas/toxicidade , Animais , Fígado/metabolismo , Malondialdeído/metabolismo , Estresse Oxidativo , Estereoisomerismo , Peixe-Zebra
18.
Arch Environ Contam Toxicol ; 73(2): 294-300, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28528415

RESUMO

This study investigated the oxidative stress of the water accommodated fraction (WAF) of zero sulfur diesel oil and Pinghu crude oil on Sinonovacula constricta, respectively. The oxidative stress of oil pollutants on organisms was measured by typical antioxidases, such as SOD, CAT, GST, and POD. Toxicity was quantitatively evaluated by combining IBR (integrated biomarker responses). Results demonstrated that different concentrations of oil caused different degrees of induction to the four antioxidases. Compared with the control group, all test groups presented enzyme induction or inhibition during exposure period. Combined with the calculated IBR values, the high-concentration group of the zero sulfur diesel oil could cause the largest biological effect changes, which reflected its high oxidative stress. The zero sulfur diesel oil had stronger toxicity than Pinghu crude oil.


Assuntos
Bivalves/fisiologia , Estresse Oxidativo , Petróleo/toxicidade , Testes de Toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Biomarcadores/metabolismo
19.
Environ Pollut ; 212: 18-26, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26840512

RESUMO

Difenoconazole is a widely used triazole fungicide and has been reported to have negative impacts on zebrafish embryos. To investigate the mechanism of its developmental toxicity, zebrafish embryos were exposed to 0.5 and 2.0 mg/L difenoconazole for 96 h. The morphological and physiological indicators of embryo development were tested. The total cholesterol (TCHO) level, triglyceride (TG) level and malondialdehyde (MDA) content were measured at 96 hpf (hours post-fertilization). In addition, the transcription of genes related to embryo development, the antioxidant system, lipid synthesis and metabolism was quantified. Our results showed that a large suite of symptoms were induced by difenoconazole, including hatching regression, heart rate decrease, growth inhibition and teratogenic effects. 0.5 mg/L difenoconazole could significantly increase the TG content of zebrafish embryos at 96 hpf, while no apparent change in the TCHO and MDA level was observed post 96 h exposure. Q-PCR (quantitative real-time polymerase chain reaction) results showed that the transcription of genes related to embryonic development was decreased after exposure. Genes related to hatching, retinoic acid metabolism and lipid homeostasis were up-regulated by difenoconazole.


Assuntos
Dioxolanos/toxicidade , Fungicidas Industriais/toxicidade , Triazóis/toxicidade , Peixe-Zebra/embriologia , Animais , Embrião não Mamífero/anormalidades , Embrião não Mamífero/efeitos dos fármacos , Embrião não Mamífero/metabolismo , Desenvolvimento Embrionário/efeitos dos fármacos , Desenvolvimento Embrionário/genética , Metabolismo dos Lipídeos/efeitos dos fármacos , Regulação para Cima , Peixe-Zebra/anormalidades , Peixe-Zebra/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...