RESUMO
Via utilizing the mixed-ligand method, a novel coordination polymer (CP) containing Cu(II) has been prepared by self-assembly at the ambient temperature, and its chemical formula is {[Cu(BINDI)0.5 (bpe)]·3H2O} n (1, bpe = 1,2-bis(4-pyridyl)ethylene and H4BINDI = N,N'-bis(5-isophthalic acid) naphthalenediimide). For the treatment of the tuberculosis, its biological function was evaluated as well. The CFU assay was performed to determine the bacterial numbers of the Mycobacterium in alveolar macrophages. In addition to this, the ropA gene of the Mycobacterium in alveolar macrophages was also detected through the real time RT-PCR method. Only the oxygen atoms on the metal complex are identified to be able to interact with the probe protein by molecular docking simulation.
Assuntos
Cobre/farmacologia , Macrófagos Alveolares/microbiologia , Mycobacterium tuberculosis/efeitos dos fármacos , Polímeros/farmacologia , Proteínas de Bactérias/análise , Proteínas de Bactérias/genética , Contagem de Colônia Microbiana , Cobre/química , Cobre/uso terapêutico , Expressão Gênica/efeitos dos fármacos , Ligantes , Conformação Molecular , Simulação de Acoplamento Molecular , Mycobacterium tuberculosis/genética , Oxigênio , Polímeros/química , Polímeros/uso terapêutico , Reação em Cadeia da Polimerase em Tempo Real , Tuberculose/tratamento farmacológico , Tuberculose/microbiologiaRESUMO
The radiation spectrum of pyrotechnics' burning flame was analyzed using transient spectrum radiometer. The working principle of multi-spectral thermometry was described. Combined with the radiation spectrum of pyrotechnics' burning flame, the multi-spectral thermometer system was designed which had twelve working channels. The tester can choose the right working channels to calculate according to the radiation spectrum of the flame to be tested. The system is composed by optics part, electronic part, data acquisition part and data processing part. In this paper, the emissive power of black powder's flame has been tested using the multi-spectral thermometer system. The burning flame temperature-time curve was showed after iteration calculation Experiments indicate that the multi-spectral thermometer system can be well used to measure the flame temperature of pyrotechnics based on analyzing the emissive power when choosing the right working channels. This method lays a foundation for the research of combustion output characteristics of pyrotechnics.
