Microplastics drive microbial assembly, their interactions, and metagenomic functions in two soils with distinct pH and heavy metal availability.

Microplastics (MPs) have emerged as widely existing global environmental concerns in terrestrial ecosystems. However, the mechanisms that how MPs are affecting soil microbes and their metagenomic functioning is currently uncertain. Herein, we investigated the response mechanisms of bacterial and fungal communities as well as the metagenomic functions to the addition of MPs in two soils with distinct pH and heavy metals. In this study, the acidic soil (Xintong) and the neutral soil (Huanshan) contaminated by heavy metals were incubated with Polyvinyl Chloride (PVC) MPs at ratios of 2.5% and 5% on 60 and 120 days. We aimed to evaluate the responding, assembly, and interactions of the metagenomic taxonomy and function. Results showed that only in the acidic soil, PVC MPs significantly increased soil pH and decreased CaCl2-extractable heavy metals, and also reduced bacterial alpha diversity and interaction networks. The relative proportions of Proteobacteria and Bacteroidota in bacteria, and Mortierellomycota in fungi, were increased, but Chloroflexi and Acidobacteriota in bacteria, Ascomycota and Basidiomycota in fungi, were significantly decreased by PVC MPs. Metagenomic functions related to C cycling were repressed but the nutrient cycles were enriched with PVC MPs. In conclusion, our study suggests that the addition of PVC MPs could shift soil microbial community and metagenomic functioning, as well as increasing soil pH and reduced heavy metal availability.

Effects of sports environment design on children s fundamental movement skills / 中国学校卫生

Objective@#Based on Newell s Model of Constraints, the present study aims to explore the effects of sports environment design on children s fundamental movement skills, and to provide certain theoretical and practical guidance for promoting children s movement development and carrying out sports activities.@*Methods@#From March to June 2022, using the method of cluster sampling, 153 typical developmental children from a private kindergarten in Xi an were selected, and were randomly assigned to the regular activity group, sports division A group, and sports division B group for a period of 12 weeks (twice a week, 1 hour each time) experimental intervention. Among them, the regular activity group engaged in regular physical activities according to the kindergarten plan; the sports division A group participated in daily physical activities after the sports division; and the sports division B group engaged in structured and autonomous physical activities after the sports division. Motorische Basiskompetenzen in Kindergarten (MOBAK-KG) scale was used to assess the level of fundamental movement skills. Chi square test, one way analysis of variance and repeated measures analysis of variance were used to test the effects of sports environment design on children s fundamental movement skills.@*Results@#After the experiment, children s manipulative skills，mobility skills, and fundamental movement skills improved significantly ( P ＜0.01); group and test time had a significant interaction on children s fundamental movement skills [ F (2,150) =113.07, P ＜0.01, η 2=0.60]，the posttest score of fundamental movement skills of children in group B of sports division (12.08±1.82) was significantly higher than that of group A of sports division (10.71±2.56), regular activity group (8.57±4.16).@*Conclusion@#The sports environment design under the constraint model perspective can effectively promote the development of children s fundamental movement skills. Kindergartens, families and communities should coordinate to promote the development of children s fundamental movement skills.

Co-pyrolysis of sewage sludge and metal-free/metal-loaded polyvinyl chloride (PVC) microplastics improved biochar properties and reduced environmental risk of heavy metals.

Co-pyrolysis of sewage sludge and plastics have been utilized for producing biochars as a strategy to fix plastic pollution. However, comparative studies on the characteristics and environmental risk of heavy metals in biochars obtained by the co-pyrolysis of sludge and microplastic with/without metal additives are seldom. Here we demonstrated the effects of simulated co-pyrolysis (at 400 °C) of sewage sludge and metal-free or metal-loaded polyvinyl chloride (PVC) microplastics at different mass ratios (1:0, 19:1, 3:1, 1:3, sewage sludge: PVC (w/w)) respectively. Results revealed that co-pyrolysis of metal-loaded PVC and sewage sludge resulted in higher electrical conductivity, ash content, and an acidic pH of biochars as compared to the co-pyrolysis of metal-free PVC and sewage sludge. Addition of metal-loaded PVC increased total concentrations of calcium (Ca), magnesium (Mg), cadmium (Cd), and lead (Pb) in biochars, but reduced the bioavailability of Cd, chromium (Cr), nickel (Ni), and zinc (Zn) in biochars. Analysis of chemical speciation showed that heavy metals (except Pb) in biochars derived from co-pyrolysis of sewage sludge and metal-loaded PVC had higher percentage of more stable fraction (residual fraction) and lower potential ecological risk index (RI) value. S1AP3 (sludge: metal-loaded PVC = 1:3) biochar had the lowest environmental risk based on RI value (14.41). To sum up the present study suggests that the addition of metal-loaded PVC microplastic in sewage sludge had a positive impact on the immobilization of heavy metals during co-pyrolysis process.

Performance and mechanisms for remediation of Cd(II) and As(III) co-contamination by magnetic biochar-microbe biochemical composite: Competition and synergy effects.

Contaminations by heavy metals in the environment always exist as a mixture of both metal and metalloid. Thus, it is a challenge to simultaneously remove both components due to their adverse chemical behaviors. Herein, effective cadmium (Cd) and arsenic (As) removal in aqueous solution was achieved by use of a novel composite, which was synthesized by Bacillus sp. K1 loaded onto Fe3O4 biochar (MBB). The combination with Bacillus sp. K1 provided new biosorption sites such as amine and hydroxyl groups in the composite surface, which significantly increasing the removal capability of Cd(II) by 230% when compared with the raw magnetic biochar. Both competition and synergy effects were found in binary system. Adsorption of As(III) extended active sites for capturing Cd(II), which appeared on the surface of the MBB as type B ternary surface complexes. The maximum adsorption capacity of Cd(II) and As(III) reached 25.04 and 4.58 mg g-1 in a binary system, respectively. In summary, this environmentally friendly composite is promising for simultaneous Cd(II) and As(III) remediation.

A novel calcium-based magnetic biochar reduces the accumulation of As in grains of rice (Oryza sativa L.) in As-contaminated paddy soils.

The present study used calcium-based magnetic biochar (Ca-MBC), a novel material made through pyrolyzing rice straw impregnated with iron oxide (Fe3O4) and calcium carbonate (CaCO3) under oxygen-limited conditions, to reduce arsenic (As) accumulation in rice plants (Oryza sativa L.) through a 130-day pot experiment. The BCR (European Community Bureau of Reference) sequential extraction confirmed that Ca-MBC decreased the unstable fraction of As through transforming to the stable fraction at both tillering stage and maturity. The addition of Ca-MBC decreased while the pristine biochar increased the concentrations of NH4H2PO4- and BCR-extracted As. The µ-XRF test revealed that iron oxide on the Ca-MBC played an important role in decreasing As bioavailability. The addition of Ca-MBC greatly decreased As concentration in rice grains, mainly due to (1) the decreases in bioavailability of As in soil and (2) adsorption of As in pore water by Ca-MBC; and (3) the enhanced formation of iron plaque that acted as a barrier for plant As uptake. Furthermore, the addition of Ca-MBC at 1% but not 2% promoted plant growth. The results suggest that Ca-MBC can be used as an efficient material to lower As accumulation in grains and promote plant growth in rice paddy fields.

Zeolite-supported nanoscale zero-valent iron for immobilization of cadmium, lead, and arsenic in farmland soils: Encapsulation mechanisms and indigenous microbial responses.

Zeolite-supported nanoscale zero-valent iron (Z-NZVI) has great potential for metal(loid) removal, but its encapsulation mechanisms and ecological risks in real soil systems are not completely clear. We conducted long-term incubation experiments to gain new insights into the interactions between metal(loid)s (Cd, Pb, As) and Z-NZVI in naturally contaminated farmland soils, as well as the alteration of indigenous bacterial communities during soil remediation. With the pH-adjusting and adsorption capacities, 30 g kg-1 Z-NZVI amendment significantly decreased the available metal(loid) concentrations by 10.2-96.8% and transformed them into strongly-bound fractions in acidic and alkaline soils after 180 d. An innovative magnetic separation of Z-NZVI from soils followed by XRD and XPS characterizations revealed that B-type ternary complexation, heterogeneous coprecipitation, and/or concurrent redox reactions of metal(loid)s, especially the formation of Cd3(AsO4)2, PbFe2(AsO4)2(OH)2, and As0, occurred only under specific soil conditions. Sequencing of 16S rDNA using Illumina MiSeq platform indicated that temporary shifts in iron-resistant/sensitive, pH-sensitive, denitrifying, and metal-resistant bacteria after Z-NZVI addition were ultimately eliminated because soil characteristics drove the re-establishment of indigenous bacterial community. Meanwhile, Z-NZVI recovered the basic activities of bacterial DNA replication and denitrification functions in soils. These results confirm that Z-NZVI is promising for the long-term remediation of metal(loid)s contaminated farmland soil without significant ecotoxicity.

A novel calcium-based magnetic biochar is effective in stabilization of arsenic and cadmium co-contamination in aerobic soils.

This study developed a novel calcium-based magnetic biochar by pyrolysing rice straw mixed with calcium carbonate and iron oxide for stabilization of contamination of multiple metals. A 160-day incubation study was conducted to investigate its performance in stabilization of cadmium and arsenic co-contamination in soil. Both biochar and Ca-MBC treatments increased soil pH, decreased the bioavailability of cadmium. Ca-MBC decreased but biochar enhanced the bioavailability of arsenic. The BCR (European Community Bureau of Reference) sequential extraction confirmed Ca-MBC facilitated the transformation of the unstable fraction of arsenic to stable fractions. The stabilization mechanisms were explored through synchrotron-based micro X-ray fluorescence and X-ray absorption near edge structure. The results show that Ca-MBC remediated the dual contamination of arsenic and cadmium through (1) elevated pH and cation exchange capacity (for Cd); (2) the formation of bi-dentate chelate and ternary surface complexes on the surface of iron oxide; (3) enhanced adsorption ability of porous biochar. In addition, Ca-MBC increased the abundance and diversity of bacterial community, and modified the relative abundances of bacterial taxa, leading to a shift of the composition. These new insights provide valuable information for stabilization of co-contamination of arsenic and cadmium in soil using the potential material Ca-MBC.

Simultaneous adsorption of Cd(II)andAs(III)by a novel biochar-supported nanoscale zero-valent iron in aqueous systems.

Biochar-supported nanoscale zero-valent iron (nZVI-BC) is a promising material for Cd(II) and As(III) removal from aqueous systems. In this study, simplified nZVI-BC composites were successfully synthesized and characterized via scanning electron microscopy (SEM), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectrometry (XPS), and Fourier transform infrared spectroscopy (FTIR) to understand the underlying adsorption mechanism. SEM and FTIR confirmed that nZVI particles were distributed evenly on the biochar surface. XRD and XPS revealed that metal ions were separated from solutions via electrostatic adsorption, complexation, oxidation, precipitation/co-precipitation, and the formation of type B ternary surface complex. Batch experiments showed that nZVI-BC (1:1) had a high removal efficiency in a wide pH range of 5.0-8.0 for Cd(II) and 3.0-8.0 for As(III), the maximum Cd(II) and As(III) adsorption capacities were 33.81 and 148.5 mg/g within 2 and 1 h, respectively. Additionally, synergisticeffects considerably enhanced the adsorption capacity of nZVI-BC(1:1) in mixed adsorption systems, the adsorption capacities of Cd(II) and As(III) reached 179.9 and 158.5 mg/g, respectively. Hence, nZVI-BC(1:1) is an ideal candidate for Cd(II) and As(III) pollution treatment.

A comprehensive mitigation strategy for heavy metal contamination of farmland around mining areas - Screening of low accumulated cultivars, soil remediation and risk assessment.

A three-year field test was conducted in an area surrounding past mining activity (mining area) to investigate the value of a novel comprehensive remediation strategy for Cd and Pb contamination, which included screening of low accumulated vegetable cultivars that take up Cd and Pb less than normal cultivars, in situ soil remediation using different soil amendments, and health risk assessment that evaluates the possibility of safe consumption for the vegetables. Results showed that cultivar Huoqing 91-5C of which vegetable was selected as a low accumulator of Cd and Pb in a soil contaminated with 0.5 mg kg-1 and 8180 mg kg-1 total Cd and Pb concentrations, respectively. Addition of 20 t ha-1 of biochar with 2 t ha-1 of calcium superphosphate in 10 cm depth could decrease available Cd and Pb by 70% and 85% after 1 year, respectively. Following treatments, hazard quotients for adults and children were below 1, indicating that the vegetables grown were safe for human consumption. The total cost of remediation was $3885 ha-1, so the cost of the remediation of the combination of Cd and Pb was economic in this mining area.

Ten-year regional monitoring of soil-rice grain contamination by heavy metals with implications for target remediation and food safety.

Farmland soil heavy metal contamination could pose potential risks to ecosystems, food safety and human health ultimately. Regional researches on the long-term monitoring of heavy metals in a soil-rice grain system, changed with environmental policy adjustment, have been hindered by limited detailed data. In this study, we collected 169 paired paddy rice grain and corresponding soil samples from a former intensive electronic-waste dismantling region to survey the current status of heavy metal contamination, and to reveal the temporal trends over the past decade based on the previous data obtained in 2006 and 2011. Moderate contaminations of Cd, Cu, Zn and Ni were observed in soil currently. Furthermore, 20.7% of rice grain samples exceeded the Cd threshold value. Cd, Cu, Zn and Pb shared the similar spatial distribution pattern with higher concentrations in northwest, which were contrary to Cr, Ni and As. Risk assessment indicated that much attention is required for the carcinogenic risk of Cr, Cd and As and non-carcinogen risk of Cr. Combining the spatial distribution of heavy metals in soil and rice grains, and the potential ecological risks, with the human health risks, the middle-west rice paddies were identified and proposed as priority areas. Percentage of soil Pb, Cd and Zn decreased in most area and slightly increased in northwest and east. Cu decreased in southwest and increased in central part, while Ni slightly increased in the whole region between 2006 and 2016. With the scrutiny of strict environmental policy, Cd still remained relatively constant levels in soil and rice grains during the last decade, which confirmed that the heavy metals were persisted over the long duration. Target sustainable and ongoing green remediation methods should be adopted urgently in specific area to guarantee food safety and human health for local residents.

A Multiobjective Evolutionary Algorithm Based on Structural and Attribute Similarities for Community Detection in Attributed Networks.

Most of the existing community detection algorithms are based on vertex connectivity. While in many real networks, each vertex usually has one or more attributes describing its properties which are often homogeneous in a cluster. Such networks can be modeled as attributed graphs, whose attributes sometimes are equally important to topological structure in graph clustering. One important challenge is to detect communities considering both topological structure and vertex properties simultaneously. To this propose, a multiobjective evolutionary algorithm based on structural and attribute similarities (MOEA-SA) is first proposed to solve the attributed graph clustering problems in this paper. In MOEA-SA, a new objective named as attribute similarity is proposed and another objective employed is the modularity . A hybrid representation is used and a neighborhood correction strategy is designed to repair the wrongly assigned genes through making balance between structural and attribute information. Moreover, an effective multi-individual-based mutation operator is designed to guide the evolution toward the good direction. The performance of MOEA-SA is validated on several real Facebook attributed graphs and several ego-networks with multiattribute. Two measurements, namely density and entropy , are used to evaluate the quality of communities obtained. Experimental results demonstrate the effectiveness of MOEA-SA and the systematic comparisons with existing methods show that MOEA-SA can get better values of and in each graph and find more relevant communities with practical meanings. Knee points corresponding to the best compromise solutions are calculated to guide decision makers to make convenient choices.

Zeolite-supported nanoscale zero-valent iron: New findings on simultaneous adsorption of Cd(II), Pb(II), and As(III) in aqueous solution and soil.

Nanoscale zero-valent iron (NZVI) has a high adsorption capacity for heavy metals, but it forms aggregates easily. In this study, zeolite-supported nanoscale zero-valent iron (Z-NZVI) was synthesized from a simplified liquid-phase reduction of iron(III) salts which simultaneously adsorbed As(III), Cd(II) and Pb(II) from aqueous solution and soil. Scanning electron micrographs showed that aggregation was eliminated and the NZVI evenly dispersed onto the surface of zeolite. FTIR spectra reveal that NZVI was protected from oxidization on the surface of Z-NZVI. XRD and XPS patterns confirmed the formation of Cd(OH)2, Pb0, and FeAsO4 in Z-NZVI after adsorption. The experimental maximum adsorption capacity of Z-NZVI was 11.52mg As(III)/g, 48.63mg Cd(II)/g, and 85.37mg Pb(II)/g at pH 6, respectively, much higher than that of zeolite. Batch experiments indicate that various adsorption mechanisms including electrostatic adsorption, ionic exchange, oxidation, reduction, co-precipitation, and complexation coexisted with the selected heavy metals. Due to the formation of multiphase compounds on the Z-NZVI, the synergy and competition among heavy metals were concurrent. Most arsenic, cadmium and lead in the soil samples were immobilized after mixing with 30g/kg Z-NZVI. These results suggest that Z-NZVI has great potential for treating water and soil multi-contaminated with heavy metals.

First "charosphere" view towards the transport and transformation of Cd with addition of manure derived biochar.

The specific area between biochar and soil surfaces is termed the "charosphere", which is analogous to the rhizosphere between plant roots and soil. Physicochemical properties of charosphere soil differ from natural soil. A double-layer mesh bag experiment was conducted to separate the charosphere soil (>2 mm) from biochar (derived from manure pyrolyzed at 300, 500, 700 °C) and the bulk soil. A 95-day experiment determined the availability, total concentration and speciation of Cd in charosphere soil and the total and available Cd in biochar. The availability of Cd decreased in the charosphere soils. Total Cd concentration increased in the biochars but decreased in charosphere soil, suggesting that Cd might transfer from charosphere soil to biochar. Cd in the acid soluble fraction decreased by 25-40% in different biochars while it increased in residue fraction, indicating that Cd tends to be more stable in charosphere soil. Instrumental analysis using SEM, FTIR were performed to provide further evidence of the transport of Cd and to enable interpretation of the mechanisms involved. The phenomena that Cd concentrations change markedly in the charosphere inspires a novel research perspective towards the study of transport and transformation of heavy metals in soil after biochar application.