RESUMO
Two rapid (pilot) methods for the determination of blood microflora are compared. One of them is based on bacterioscopy, the other on culturing of bacteria. Use of these methods helps increase the detection rate of microorganisms in the blood from 34.8 to 59.6% in a shorter time, 1-3 days instead of 5-7. No additional staff or sophisticated equipment and tests kits are needed for the tests. The methods are recommended for research and clinical laboratories.
Assuntos
Técnicas Bacteriológicas , Sangue/microbiologia , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Meios de Cultura , Humanos , Projetos Piloto , Fatores de TempoRESUMO
The possibility of conducting intraoperative reinfusion of blood in coloproctology is appraised on the basis of complex microbiological, cytological, and biochemical study of blood of patients who underwent operation for various diseases of the large intestine. It was found that blood escaping into the abdominal cavity during operations on the large intestine is hemolized to a great measure and contains many bacteria, and therefore such blood cannot be used for transfusion. At the same time, if blood is collected directly from the pulsating vessel its contamination, marked distortions of its formed elements, and hemolysis above admissible values can be avoided. The dependence on the time of blood collection, whether before or after the intestine is opened, has not been established.