[Thrombolytic effect of urokinase upon various methods of administration into the body]. / Tromboliticheskoe deistvie urokinazy pri raznykh sposobakh ee vvedeniia v organizm.

A preparation of urokinase, obtained from human kidney cell culture, was administered into rats at a single dose of 5,000-10,000 U/200 g of body mass in a variety of ways using intravenous, intraperitoneal and subcutaneous inoculations. After intraperitoneal and subcutaneous administrations an increase of fibrinolytic activity in blood was more long-term although less distinct; the phase of reactive hypercoagulation was only slightly detected within 24 hrs after these procedures. Thrombin-produced provocation of thrombosis led to a lesser ratio of death in these animals as compared with the animals group administered intravenously. However, thrombolytic effect of similar doses of urokinase was the highest in intravenous administration. The fibrinolytic activity correlated well with content of the antigen (enzyme) in blood but not with the antiplasmin content in all the procedures used.

[Determination of the enzyme activity of the fibrinolytic system using fibrinogen conjugated with peroxidase]. / Opredelenie aktivnosti fermentov fibrinolitecheskoi sistemy s ispol'zovaniem fibrinogena, kon"iugirovannogo s peroksidazoi.

The authors suggest a method for enzymic fibrinolytic activity measurements, based on the detection in the solution of degradation products of fibrin, prelabeled with peroxidase. The method is highly sensitive, permits the detection in solution of as little as 0.01 MU per ml of plasminogen activator. As the basic method, it can be employed for measurements of plasminogen activator, plasminogen activator inhibitors, and plasmin inhibitors in biologic fluids, blood plasma including. Since the method is simple and the majority of its steps may be automated, it can be used in clinical laboratories.

[Effects of tissue-type plasminogen activator from a culture of calf kidney cells on hemostasis and fibrinolysis in experimental nephritis]. / Vliianie aktivatora plazminogena tkanevo tipa iz kul'tury kletok pochki telenka na gemostaz i fibrinoliz pri éksperimental'nom nefrite.

The plasminogen activator 960 IU/mg protein activity isolated from cultured fluid of the calf kidney cells was introduced to albino rats (180-200 g) with experimental Heynmann nephritis every day during 4 days. Nephritis caused activation of haemostasis and inhibition of fibrinolysis in the blood. There was increased excretion of the fibrin, fibrinogen degradation products in urine as a results of the local fibrin deposition in diseased kidneys. The fibrinolytic activity of the cortical zone of kidney was markedly decreased. The plasminogen activator, infused to experimental animals, resulted in normalization of the altered indexes.

[Fibrinolytic and thrombolytic properties of a plasminogen activator isolated from the culture broth of calf kidney cells]. / Fibrinoliticheskie i tromboliticheskie svoistva aktivatora plazminogena, vydelennogo iz kul'tural'noi zhidkosti kletok pochki teliat.

Plasminogen activator isolated from cultural liquid of calf renal cells is an enzyme having a fibrinolytic action in vitro and in vivo, provoking a demonstrable thrombolytic effect, which depends on the drug dose, thrombus size and animal's body weight.

[Plasminogen activator from a kidney cell culture]. / Aktivator plazminogena iz kul'tury kletok pochki.

Plasminogen activator (PA) isolated from kidney's cells culture (doses 10 to 5000 IE/kg) induced lysis of experimental thrombi. Lysis time decreased from 214 to 23 min after PA infusion in doses 10 to 700 IE/kg, further doses decrease from 700 to 5000IE/kg does not accelerate lysis time of thrombi. PA infusion in doses mentioned increases the activator activity of plasma and euglobulin lysis time, but does not change the fibrinolytic activity, antiplasmin content and fibrinogen concentration.