RESUMO
OBJECTIVE: To illustrate the role of FOXF2 in the aggravation of the progression of non-small cell lung cancer (NSCLC) by targeting long non-coding RNA (lncRNA) H19 to down-regulate the gene of phosphate and tensin homolog deleted on chromosome ten (PTEN). PATIENTS AND METHODS: The relative levels of FOXF2 and H19 in NSCLC tissues and adjacent normal tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between the expression levels of FOXF2 and H19 was analyzed. Kaplan-Meier curves were depicted for uncovering the prognostic value of FOXF2 in NSCLC patients. The proliferative and migratory abilities of A549 cells influenced by FOXF2 were also assessed. The interaction between FOXF2 and H19 was evaluated through chromatin immunoprecipitation (ChIP) assay and Western blot, so did the association between H19 and PTEN. Finally, rescue experiments were conducted to explore the role of FOXF2/H19/PTEN axis in regulating the viability and migration of A549 cells. RESULTS: FOXF2 and H19 were upregulated in NSCLC and a positive correlation was observed between the two genes. High level of FOXF2 indicated a worse prognosis in NSCLC patients. The knockdown of FOXF2 attenuated the proliferative and migratory abilities of A549 cells. FOXF2 could bind to the promoter region of H19 and accelerated its transcription. Moreover, H19 could recruit EZH2 to bind to PTEN. The overexpression of H19 could reverse the regulatory effects of FOXF2 on the viability and migration of A549 cells. CONCLUSIONS: FOXF2 was upregulated in NSCLC. It accelerated the proliferative and migratory abilities of the NSCLC cells by targeting H19 to downregulate PTEN, thus aggravating the progression of NSCLC.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Fatores de Transcrição Forkhead/metabolismo , Neoplasias Pulmonares/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , RNA Longo não Codificante/metabolismo , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Fatores de Transcrição Forkhead/genética , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , PTEN Fosfo-Hidrolase/genética , RNA Longo não Codificante/genética , Células Tumorais CultivadasRESUMO
20 patients with intracranial A.V. malformation with orbital involvement were examined by B-Scan (Real- time ophthascanB). colour doppler (Acason 128) and CT scan. These patients were confirmed in clinically and other examination. Ultrasonography Showed dilated superior ophthalmic vein and its pulsating which are Synchroniged with the heart beating; the echo-free cavity changed in shape upon compression with the probe and some thickened extraocular muscles. The flowing counter pulsating and volume of blood stream were showed clearly in colour doppler. CT scan showed dilated superior ophthalmic vein, thickened extraocular muscles and dilate shadow of cavernous sinus. If strengthen scan to be done, the malformation of cerebrum artery and vein can be showed clearly. These examination are of value in diagnosis. The rate of ultrasound and CT scan for the differential-diagnosis was discussed.
