Chronic upper back and rib pain in a healthy man: re-examining the cause.

INTRODUCTION Back and rib pain is a common presentation in primary care practice. Although most cases are secondary to non-specific musculoskeletal pain, it is essential for clinicians to identify patients presenting with life-threatening pathologies. AIM This case report serves as a reminder to clinicians to reconsider their initial diagnosis when a patient's pain fails to improve, while considering life-threatening pathologies. CASE HISTORY We describe a 44-year-old man from India who presents to his general practitioner with a 2-week history of rib and upper back pain. He was initially diagnosed with non-specific musculoskeletal pain. However, after representing twice 2 months later due to persistent pain and due to the uncertainty about his condition, he was investigated with different imaging modalities. It was discovered on bone scan that he had osteolytic lesions in the right 11th rib and T2 vertebrae. As the cause of his osteolytic lesions were unclear, he was referred to different specialists. Skeletal tuberculosis was suspected when one of his specialists discovered his recent visit to India, a tuberculosis-endemic country. This reminded the specialist of the possible risks of the patient's background and its association with his symptoms. Bone biopsy of his lytic lesions revealed Mycobacterium tuberculosis, consistent with skeletal tuberculosis. DISCUSSION Revisiting the diagnosis of back and rib pain while considering other obscure and urgent pathologies is essential if a patient fails to improve clinically. Clinicians should focus on aspects of their clinical assessment to explore these pathologies, enabling earlier recognition of the disease.

Impact of nicotinamide mononucleotide on transplanted mouse ovarian tissue.

Ovarian tissue cryopreservation and future transplantation is the only strategy to preserve the fertility of young female adolescent and prepubertal patients. The primary challenge to ovarian graft longevity is the substantial loss of primordial follicles during the period of ischaemia post-transplantation. Nicotinamide mononucleotide (NMN), a precursor of the essential metabolite NAD+, is known to reduce ischaemic damage. Therefore, the objective of the current study was to assess the impact of short- and long-term NMN administration on follicle number and health following ovarian tissue transplantation. Hemi-ovaries from C57Bl6 mice (n = 8-12/group) were transplanted under the kidney capsule of bilaterally ovariectomised severe combined immunodeficient (SCID) mice. Recipient mice were administered either normal drinking water or water supplemented with NMN (2 g/L) for either 14 or 56 days. At the end of each treatment period, ovarian transplants were collected. There was no effect of NMN on the resumption of oestrous or length of oestrous cycles. Transplantation significantly reduced the total number of follicles with the greatest impact observed at the primordial follicle stage. We report that NMN did not prevent this loss. While NMN did not significantly impact the proportion of apoptotic follicles, NMN normalised PCNA expression at the primordial and intermediate stages but not at later stages. In conclusion, NMN administration did not prevent ovarian follicle loss under the conditions of this study.