RESUMO
AnnongS-1, a thermo-sensitive genic male-sterile (TGMS) rice line, has a new TGMS gene. Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5. In our previous studies based on an F(2) population from the cross between AnnongS-1 and Nanjing11, tms5 was mapped on chromosome 2. Recently, a RIL (recombinant inbred line) population from the same cross was developed and used for the fine mapping of the tms5 gene. Molecular marker techniques combined with BSA (bulked segregant analysis) were used. As a result, two AFLP markers (AF10, AF8), one RAPD marker (RA4), one STS marker (C365-1), one CAPs marker (G227-1) and four SSR markers (RM279, RM492, RM327, RM324) were found to be closely linked to tms5 gene. The DNA sequences of the RFLP marker of C365 and G227 were found in GenBank, and on the basis of these sequences, many primers were designed to amplify the two parents and their RIL population plants. Finally, the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1.04 cM from C365-1 and 2.08 cM from G227-1.
Assuntos
Mapeamento Cromossômico , Genes de Plantas/genética , Genes Recessivos , Infertilidade/genética , Oryza/genética , Cruzamentos Genéticos , Oryza/crescimento & desenvolvimento , Plantas Geneticamente Modificadas , Pólen/química , Pólen/genética , Polimorfismo de Fragmento de Restrição , Técnica de Amplificação ao Acaso de DNA Polimórfico , Sitios de Sequências Rotuladas , TemperaturaRESUMO
OBJECTIVE: To study the effect of Chengqi prescription in preventing hepatic ischemia/reperfusion injury after liver transplantation in rats. METHODS: Rat in situ liver transplantation model was used, donor liver was preserved in Ringer's solution for 4 hrs under 4 degrees C, then divided randomly into Chinese medicine group (Chengqi prescription), polymyxin B group, mannitol group, normal saline group and sham operation control group, each group further subdivided into two subgroups: 1 hr group and 4 hrs group after transplantation. The serum ALT and liver injury index was determined. RESULTS: In preventing hepatic ischemia/reperfusion injury after liver transplantation, the Chengqi prescription was polymyxin B showed more effective than that of mannitol and normal saline, and the effect of Chengqi prescription was higher than that of polymyxin B (P < 0.05). CONCLUSION: Chengqi prescription was effective in preventing hepatic ischemia/reperfusion injury after liver transplantation in rat.
