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Genet Mol Res ; 11(3): 2608-19, 2012 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-22869077

RESUMO

Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-treatment with trichlorphon in two-dimensional gel electrophoresis through mass spectrometry and protein sequencing. We found functional proteins that are responsible for signal transduction (pkaap and dual specificity tyrosine-phosphorylation-regulated kinase), immunity (hemolectin), synthesis and decomposition (twinstar, cathepsin B, RE66325p), oxidative stress metabolism (glutathione S transferase and CG7320), energy metabolism (Act57B), and cytoskeleton formation (actin). These proteins appear to be involved in the resistance response to trichlorphon.


Assuntos
Proteínas de Insetos/metabolismo , Resistência a Inseticidas/efeitos dos fármacos , Inseticidas/farmacologia , Proteínas Mutantes/metabolismo , Tephritidae/efeitos dos fármacos , Tephritidae/metabolismo , Triclorfon/farmacologia , Sequência de Aminoácidos , Animais , Eletroforese em Gel Bidimensional , Proteínas de Insetos/química , Proteínas de Insetos/classificação , Larva/efeitos dos fármacos , Larva/metabolismo , Dados de Sequência Molecular , Proteínas Mutantes/química , Proteínas Mutantes/classificação
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