Anomaly traffic detection based on feature fluctuation for secure industrial internet of things.

The detection of anomaly traffic in internet of things (IoT) is mainly based on the original binary data at the traffic packet level and the structured data at the session flow level. This kind of dataset has a single feature extraction method and relies on prior manual knowledge. It is easy to lose critical information during data processing, which reduces the validity and robustness of the dataset. In this paper, we first construct a new anomaly traffic dataset based on the traffic packet and session flow data in the Iot-23 dataset. Second, we propose a feature extraction method based on feature fluctuation. Our proposed method can effectively solve the disadvantage that the data collected in different scenarios have different characteristics, which leads to the feature containing less information. Compared with the traditional anomaly traffic detection model, experiments show that our proposed method based on feature fluctuation has stronger robustness, can improve the accuracy of anomaly traffic detection and the generalization ability of the traditional model, and is more conducive to the detection of anomalous traffic in IoT.

Recombinant SFRP5 protein significantly alleviated intrahepatic inflammation of nonalcoholic steatohepatitis.

BACKGROUND: Secreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine modulating metabolism dysfunction. This study aims to observe the effect of recombinant SFRP5 protein on nonalcoholic steatohepatitis (NASH). METHODS: We set up a prokaryotic expression system and purified the recombinant SFRP5 protein. Recombinant SFRP5 protein was further identified by SDS-PAGE, western blot, high performance liquid chromatography (HPLC), protein mass spectrometry and in vitro Wnt5a-binding test. NASH mouse model was induced by methionine and choline deficient diet (MCDD) for 2 weeks. SFRP5 treatment group received intraperitoneal injection with a dosage of 10µg/kg SFRP5 twice a day for 2 weeks. Saline was used as control. Inflammation and fatty lesion score of liver tissue pathology and serum transaminase level were compared. RESULTS: The purity of recombinant SFRP5 protein is 90% identified by HPLC. Its molecule size is 36,096.08 tested by mass spectrometry. Recombinant SFRP5 can specifically bind with Wnt5a which verifies its activity in vitro. The endotoxin level of this recombinant protein is 0.01EU/µg-0.1EU/µg and is suitable for animal experiment. SFRP5 can significantly improve liver inflammation (SFRP5 vs. control, 1.40 ± 0.70 vs. 2.00 ± 0.47, P < 0.05) as well as fatty lesion scores (SFRP5 vs. control, 1.40 ± 0.97 vs. 2.20 ± 0.63, P < 0.05), and lower ALT and AST levels. The mRNA expression of proinflammatory adipokines (IL-1ß, IL-6, TNFα and MCP-1) in liver was down-regulated significantly after SFRP5 intervention. Immunohistochemistry and quantitative PCR revealed a dramatically down-regulation of F4/80 in liver after SFRP5 treatment. CONCLUSIONS: Recombinant SFRP5 protein significantly alleviated NASH induced by MCDD.