RESUMO
The brp-like genes in amphioxus (Branchiostoma belcheri tsingtauense) and in zebrafish (Danio rerio) are reported. The putative brp-like proteins are orthologous to the ancestor of the human brain protein 239FB and 239AB. Previous studies showed that human brain protein 239 might play a role in central nervous system development and function. In this study, the transcripts of zebrafish brp-like gene are mainly located in the developing central neural system in embryo and larva similar to its orthologous genes in human. In contrast, the developmental expression pattern of amphioxus brp-like gene suggests that the gene might be involved in the development of the notochord, pharynx, gills and gut in amphioxus embryos and larvae. Phylogenetic analysis shows that the amphioxus brp-like gene is closer to the orthologous genes in vertebrates than those in invertebrates. The results suggest that the function of the brain protein 239 family genes may change in evolution.
Assuntos
Cordados não Vertebrados/genética , Filogenia , Proteínas de Peixe-Zebra/genética , Peixe-Zebra/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Cordados não Vertebrados/embriologia , Clonagem Molecular , Embrião não Mamífero , Regulação da Expressão Gênica no Desenvolvimento , Dados de Sequência MolecularRESUMO
We report here the expression of AmphiMdp in embryos and larvae. Faint AmphiMdp transcripts were first detected in the mesendoderm at the mid-gastrula stage and later in the somites of the early neurula. Expression remained in somites throughout the neurula and early larval stages and then disappeared from the somites starting with the most anterior somite and progressing posteriorly. At the 48-h larval stage, transcripts were detected in the developing tail bud. No transcripts were detectable in the somites of the 72-h larva. The result suggests that AmphiMdp is involved in myogenesis in amphioxus.
Assuntos
Cordados não Vertebrados/metabolismo , Fatores de Crescimento Neural/biossíntese , Sequência de Aminoácidos , Animais , Cordados não Vertebrados/embriologia , Cordados não Vertebrados/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento , Biblioteca Gênica , Hibridização In Situ , Larva/crescimento & desenvolvimento , Larva/metabolismo , Dados de Sequência Molecular , Fatores de Crescimento Neural/genética , Proteínas de Plantas/genética , Homologia de Sequência de Aminoácidos , Somitos/metabolismoRESUMO
A full-length amphioxus gamma-aminobutyric acid type-A receptor-associated protein-like 2 ( GABARAPL2) cDNA was isolated. Its sequence and developmental expression are first described in this paper. The phylogenetic analysis shows that the amphioxus GABARAPL2 and GABARAPL2 in vertebrates are highly homologous. The results of in situ hybridization show that the amphioxus GABARAPL2 gene is expressed in the neural tube, neurenteric canal, notochord, muscle and developing alimentary canal.
