Attentional bias for rejection and sad words in Chinese left-behind children with depression.

BACKGROUND: This study aimed to examine attentional bias (AB) for sad and social rejection words in Chinese left-behind children (LBC) with depression. METHOD: We investigated both stimulus specificity and components of AB in different groups using a cross-sectional design. Data were drawn from a school assessment of depression and anxiety, from which we selected LBC with depression (n = 40), LBC without depression (n = 33), a control group with depression (n = 31), and a control group without depression (n = 37). AB was measured with a dot-probe task covering two stimulus types (sad and rejection). RESULTS: The analysis of AB scores revealed a significant three-way interaction (LBC × depression × word type), F(1, 137) = 4.00, p = 0.047, η2 = 0.028, with depressed non-LBC exhibiting a significant depression × word type interaction, F(1, 66) = 4.67, p = 0.034, η2 = 0.066, while the depression × word type interaction was not significant in LBC, F(1, 71) = 0.18, p = 0.675, η2 = 0.002. Depressed children living with their parents showed AB towards sad words but not rejection words, while depressed LBC showed greater AB towards both rejection and sad words. CONCLUSIONS: The findings provide evidence that an AB towards sad information is critically involved in the depressed LBC. Compared with non-LBC depressed individuals, an AB for rejection may be involved as a risk factor in the LBC. It sheds light on the effective intervention programmes for LBC's depression and have important practical significance for reducing depression and improving the mental health of LBC.

Activating lattice oxygen in high-entropy LDH for robust and durable water oxidation.

The oxygen evolution reaction is known to be a kinetic bottleneck for water splitting. Triggering the lattice oxygen oxidation mechanism (LOM) can break the theoretical limit of the conventional adsorbate evolution mechanism and enhance the oxygen evolution reaction kinetics, yet the unsatisfied stability remains a grand challenge. Here, we report a high-entropy MnFeCoNiCu layered double hydroxide decorated with Au single atoms and O vacancies (AuSA-MnFeCoNiCu LDH), which not only displays a low overpotential of 213 mV at 10 mA cm-2 and high mass activity of 732.925 A g-1 at 250 mV overpotential in 1.0 M KOH, but also delivers good stability with 700 h of continuous operation at ~100 mA cm-2. Combining the advanced spectroscopic techniques and density functional theory calculations, it is demonstrated that the synergistic interaction between the incorporated Au single atoms and O vacancies leads to an upshift in the O 2p band and weakens the metal-O bond, thus triggering the LOM, reducing the energy barrier, and boosting the intrinsic activity.

Quality evaluation index development for nutritional management in patients with nasopharyngeal carcinoma during peri-radiotherapy.

OBJECTIVES: It is necessary to construct an evaluation index for patients with nasopharyngeal carcinoma during peri-radiotherapy to provide a reference for the evaluation of the quality of nutritional management of patients with nasopharyngeal carcinoma during peri-radiotherapy. The aim of this study was to construct a set of scientific, comprehensive, and feasible indicators for evaluating the quality of nutrition management in patients with nasopharyngeal carcinoma during peri-radiotherapy to provide a unified reference basis for objective nutritional evaluation of these patients during the peri-radiotherapy period and to provide insights to the clinical treatment and care of these patients. METHODS: A multidisciplinary research team was set up from December 2021 to April 2022. We took the three-dimensional quality structure model as the theoretical framework; based on the literature review, the first draft of the nutrition management quality evaluation index for patients with nasopharyngeal carcinoma during peri-radiotherapy was formed by a semi-structured interview. The Delphi correspondence method was used to survey 18 experts from 12 cities in China. The multidimensional analytical hierarchy process was used to determine the evaluation index and weight of nutrition management quality of patients with nasopharyngeal carcinoma during peri-radiotherapy. RESULTS: The effective questionnaire recovery rates of the two rounds of letters were 90.005% and 100%, respectively, and the expert authority coefficients were 0.906 and 0.918, respectively. The Kendall harmony coefficients of the two rounds of letters were 0.271 to 0.313 and 0.309 to 0.349, respectively. The nutrition management quality evaluation index of patients with nasopharyngeal carcinoma during peri-radiotherapy was constructed and included 3 first-level indexes, 10 second-level indexes, and 71 third-level indexes. CONCLUSION: The evaluation index of the nutrition management quality of patients with nasopharyngeal carcinoma during peri-radiotherapy is scientific and reliable, and it may have a certain guiding significance for nurses to evaluate the quality of nutrition management of these patients during this period.

Efficacy and confounding factors of CT attenuation value differences in distinguishing acute and old vertebral compression fractures: a retrospective study.

PURPOSE: To evaluate the influence of various factors on CT attenuation values (HUs) of acute and old fracture vertebra, and to determine the efficacy of HU differences (△HUs) in the differentiation of the two type of fractures. MATERIALS AND METHODS: A total of 113 acute and 71 old fracture vertebrae confirmed by MRI were included. Four HUs measured at the mid-sagittal, upper 1/3 axial, mid-axial, and lower 1/3 axial planes of each vertebra were obtained. The △HUs between fracture vertebra and its control counterpart was calculated. Receiver operating characteristic (ROC) curve analysis was used and the areas under the ROC curve (AUC) were calculated to evaluate the efficacy of HUs and △HUs. To evaluate the effect of height reduction, region, age and gender on HUs and △HUs, one-way analysis of variance, Pearson correlation analysis and t-test were used. RESULTS: The HUs and △HUs at the upper 1/3 axial plane achieved the highest AUCs of 0.801 and 0.839, respectively. The HUs decreased gradually from Thoracic to Lumbar in control group of acute fracture. While no significant differences were found in the HUs among the 3 localizations in both fracture groups (all P > 0.05). The HUs were negatively correlated with age in all groups. The HUs of male were significantly higher than female patients in all groups (all P < 0.05). While △HU was not significantly different between males and females (all P > 0.05). CONCLUSION: The vertebral HUs at the upper 1/3 axial plane are more likely to identify acute fractures. △HUs were beneficial in eliminating interfering factors.

In-situ surface reconstruction of single-crystal (NiFe)3Se4 nano-pyramid arrays for efficient oxygen evolution.

Transition metal-based selenides (TMSe) are considered as efficient pre-electrocatalysts towards oxygen evolution reaction (OER). However, the key factor in determining the surface reconstruction of TMSe under OER condition is not yet clear. Herein, we uncover that the crystallinity of TMSe will obviously impact the conversion degree from TMSe to transition metal oxyhydroxides (TMOOH) during OER. A novel single-crystal (NiFe)3Se4 nano-pyramid array grown on NiFe foam is fabricated by a facile one-step polyol process, which exhibits an excellent OER activity and stability, only requiring 170 mV to reach a current density of 10 mA cm-2 and can sustain for more than 300 h. In situ Raman spectrum studies reveals that the single-crystal (NiFe)3Se4 is partially oxidized on its surface during OER, generating a dense heterostructure of (NiFe)OOH/(NiFe)3Se4. Benefiting from the in situ formed heterointerface, the adsorption of OER intermediates on Ni active sites calculated by density functional theory (DFT) analysis is optimized, leading to the reduced energy barrier, which accounts for the enhanced intrinsic activity. This work not only reports a novel single-crystal (NiFe)3Se4 nano-pyramid array electrocatalyst with high-efficient OER performance, but also gains a deep insight into the role of the crystallinity of TMSe on the surface reconstruction during OER.

Engineering cation vacancies in high-entropy layered double hydroxides for boosting the oxygen evolution reaction.

High-entropy layered double hydroxides (HE-LDHs) are emerging as promising electrocatalysts towards the OER due to their high-entropy effect and the cocktail effect. However, the catalytic activity and stability of HE-LDHs is, as yet, unsatisfactory. Herein, we designed FeCoNiCuZn LDHs with rich cation vacancies, which need only low overpotentials of 227, 275 and 293 mV to reach 10, 100 and 200 mA cm-2, respectively, and show almost no decay up to 200 h at 200 mA cm-2. DFT calculations validate that the cation vacancies can boost the intrinsic activity of HE-LDHs through optimizing the adsorption energy of OER intermediates.

Does Property Return Affect Seller Behavior? An Empirical Study of China's Real Estate Market.

This paper examines the relationship between property return and seller behavior and aims to test the disposition effect in China's real estate market. Using transaction data in Beijing, we find that loss properties have a lower sell propensity relative to gain properties, confirming the existence of the disposition effect. We also find that the disposition effect is more pronounced in samples with shorter holding periods. Sellers with financial constraints and popular projects are more likely to show the disposition effect. Furthermore, we find that sellers exhibit loss aversion; specifically, sellers with loss properties are likely to set a higher listing price, which provides indirect evidence for the disposition effect.

Multi-omics Analyses Provide Insight into the Biosynthesis Pathways of Fucoxanthin in Isochrysis galbana.

Isochrysis galbana is considered an ideal bait for functional foods and nutraceuticals of humans because of its high fucoxanthin (Fx) content. However, multi-omics analysis of the regulatory networks for Fx biosynthesis in I. galbana has not been reported. In this study, we report a high-quality genome assembly of I. galbana LG007, which has a genome size of 92.73 Mb, with a contig N50 of 6.99 Mb and 14,900 protein-coding genes. Phylogenetic analysis confirmed the monophyly of Haptophyta, with I. galbana sister to Emiliania huxleyi and Chrysochromulina tobinii. Evolutionary analysis revealed an estimated divergence time between I. galbana and E. huxleyi of âˆ¼ 133 million years ago. Gene family analysis indicated that lipid metabolism-related genes exhibited significant expansion, including IgPLMT, IgOAR1, and IgDEGS1. Metabolome analysis showed that the content of carotenoids in I. galbana cultured under green light for 7 days was higher than that under white light, and ß-carotene was the main carotenoid, accounting for 79.09% of the total carotenoids. Comprehensive multi-omics analysis revealed that the content of ß-carotene, antheraxanthin, zeaxanthin, and Fx was increased by green light induction, which was significantly correlated with the expression of IgMYB98, IgZDS, IgPDS, IgLHCX2, IgZEP, IgLCYb, and IgNSY. These findings contribute to the understanding of Fx biosynthesis and its regulation, providing a valuable reference for food and pharmaceutical applications.

Engineering nickel vacancies in NiCo LDH nanoarrays accelerates hydrogen evolution and oxygen evolution reactions.

Exploring efficient bifunctional electrocatalysts is crucial for constructing water splitting systems. In this work, a bifunctional catalyst, NiCo layered double hydroxide (LDH) nanosheets with nickel vacancies, was fabricated by a hydrothermal and chemical etching method, which requires 195 and 227 mV overpotentials for HER and OER to achieve 10 mA cm-2 and exhibited sustained activity for 100 h with almost no degradation. This study provides a new idea for the rational design of efficient non-precious metal catalysts with defects for water splitting.

Boron Coordination Effect in Ni-Nx Doped Graphene Catalysts on the ORR Performance Based on DFT Calculations.

The coordination atoms of metal active site in transition metal N-doped carbon single atom electrocatalysts play a vital role in dominating the catalytic performance of oxygen reduction reaction (ORR) at the cathode of fuel cells or metal-air cells. In view of weak adsorption ability of Ni active site in NiN4 -C catalysts to oxygen intermediate states, herein we introduce boron atoms with smaller electronegativity than N and C atoms to modulate the local coordination environment and electronic structures of Ni site. First-principles density functional calculations reveal that both B substitution for N atoms (NiN2 B2 -C) and B coordinating with N and C (NiN4 B8 -C) can effectively optimize the Gibbs free energy of oxygen intermediate states and hence improve the catalytic activity of the materials. In addition, we propose that the trend change in catalytic activity is mainly governed by the filling of antibonding orbitals between Ni-3d and O-2p states near the Fermi level.

Designing Zn-doped nickel sulfide catalysts with an optimized electronic structure for enhanced hydrogen evolution reaction.

Designing non-noble-metal electrocatalysts with excellent performance and economic benefits toward the hydrogen evolution reaction (HER) is extremely crucial for future energy development. In particular, the rational cationic-doped strategy can effectively tailor the electronic structure of the catalysts and improve the free energy of the adsorbed intermediate, thus enhancing HER performance. Herein we reported Zn-doped Ni3S2 nanosheet arrays supported on Ni foam (Zn-Ni3S2/NF) that were synthesized by a two-step hydrothermal process for improving HER catalysis under alkaline conditions. Remarkably, the obtained Zn-Ni3S2/NF displays excellent HER catalytic performance with an overpotential of 78 mV to reach a current density of 10 mA cm-2 and dramatic long-term stability for 18 h in 1 M KOH. In addition, the results based on the density functional theory calculations reveal that Zn dopants can modulate the electronic structure of Ni3S2 and optimize the hydrogen adsorption free energy (ΔGH*). Thus cationic-doping engineering provides an efficient method to enhance the intrinsic activities of transition-metal sulfides, which may contribute to the development of nonprecious electrocatalysts for HER.

MTTL3 upregulates microRNA-1246 to promote occurrence and progression of NSCLC via targeting paternally expressed gene 3.

Non-small cell lung cancer (NSCLC) is one of the major causes of morbidity and mortality worldwide. We aimed to investigate the role of N6-methyladenosine (m6A) methyltransferase-like 3 (METTL3) regulating microRNA-1246 (miR-1246) in the progression of NSCLC by targeting paternally expressed gene 3 (PEG3). METTL3, miR-1246, and PEG3 expression in tissues was assessed, and the predictive role of METTL3 in prognosis of patients with NSCLC was detected. NSCLC cells were relatively treated with altered expression of METTL3, miR-1246, or PEG3 to measure their roles in the proliferation, migration, invasion, apoptosis, and in vivo growth of the NSCLC cells. The RNA m6A level was determined, and the targeting relationship between miR-1246 and PEG3 was confirmed. Our results revealed that METTL3 and miR-1246 were upregulated, whereas PEG3 was downregulated in NSCLC tissues. METTL3 knockdown or PEG3 overexpression in NSCLC cells suppressed malignant behaviors of NSCLC cells. METTL3 affected the m6A modification of miR-1246, thus upregulating miR-1246 and miR-1246-targeted PEG3. The elevation of PEG3 reversed the effects of miR-1246 upregulation on NSCLC cells. This study revealed that m6A methyltransferase METTL3 affects the m6A modification of miR-1246, thus upregulating miR-1246 to promote NSCLC progression by inhibiting PEG3.

Insights into salvianolic acid B biosynthesis from chromosome-scale assembly of the Salvia bowleyana genome.

Salvia bowleyana is a traditional Chinese medicinal plant that is a source of nutritional supplements rich in salvianolic acid B and a potential experimental system for the exploration of salvianolic acid B biosynthesis in the Labiatae. Here, we report a high-quality chromosome-scale genome assembly of S. bowleyana covering 462.44 Mb, with a scaffold N50 value of 57.96 Mb and 44,044 annotated protein-coding genes. Evolutionary analysis revealed an estimated divergence time between S. bowleyana and its close relative S. miltiorrhiza of ~3.94 million years. We also observed evidence of a whole-genome duplication in the S. bowleyana genome. Transcriptome analysis showed that SbPAL1 (PHENYLALANINE AMMONIA-LYASE1) is highly expressed in roots relative to stem and leaves, paralleling the location of salvianolic acid B accumulation. The laccase gene family in S. bowleyana outnumbered their counterparts in both S. miltiorrhiza and Arabidopsis thaliana, suggesting that the gene family has undergone expansion in S. bowleyana. Several laccase genes were also highly expressed in roots, where their encoded proteins may catalyze the oxidative reaction from rosmarinic acid to salvianolic acid B. These findings provide an invaluable genomic resource for understanding salvianolic acid B biosynthesis and its regulation, and will be useful for exploring the evolution of the Labiatae.

Engineering Bimetallic NiFe-Based Hydroxides/Selenides Heterostructure Nanosheet Arrays for Highly-Efficient Oxygen Evolution Reaction.

Developing cost-effective and high-efficiency electrocatalysts toward alkaline oxygen evolution reaction (OER) is crucial for water splitting. Amorphous bimetallic NiFe-based (oxy)hydroxides have excellent OER activity under alkaline media, but their poorly electrical conductivity impedes the further improvement of their catalytic performance. Herein, a bimetallic NiFe-based heterostructure electrocatalyst that is composed of amorphous NiFe(OH)x and crystalline pyrite (Ni, Fe)Se2 nanosheet arrays is designed and constructed. The catalyst exhibits an outstanding OER performance, only requiring low overpotentials of 180, 220, and 230 mV at the current density of 10, 100, and 300 mA cm-2 and a low Tafel slope of 42 mV dec-1 in 1 m KOH, which is among the state-of-the-art OER catalysts. Based on the experimental and theoretical results, the electronic coupling at the interface that leads to the increased electrical conductivity and the optimized adsorption free energies of the oxygen-contained intermediates plays a crucial role in enhancing the OER activities. This work focusing on improving the OER performance via engineering amorphous-crystalline bimetallic heterostructure may provide some inspiration for reasonably designing advanced electrocatalysts.

Al-doped nickel sulfide nanosheet arrays as highly efficient bifunctional electrocatalysts for overall water splitting.

The development of low-cost, high-activity, durable non-precious metal bifunctional electrocatalysts is of great importance in the production of hydrogen by water electrolysis. In this work, we have prepared new Al-doped Ni3S2 nanosheet arrays grown on Ni foam (Al-Ni3S2/NF) as an excellent bifunctional electrocatalyst in the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). The Al-Ni3S2/NF electrode obtained only requires extremely low overpotentials of 86 and 223 mV for the HER and OER to achieve a current density of 10 mA cm-2 in 1 M KOH, respectively. Moreover, the electrolytic cell assembled using this electrode as both cathode and anode provides a current density of 10 mA cm-2 at an extremely low battery voltage of 1.58 V relative to that with Ni3S2/NF (1.71 V). Additionally, both experimental results and theoretical calculations reveal that the increased electrochemical active surface area and optimized intermediate adsorption free energies are responsible for the enhanced electrocatalytic performance. This work provides a promising bifunctional electrocatalyst for water electrolysis in alkaline media with broad application prospects.

[Effect of HRT on Denitrifying Phosphorus and Nitrogen Removal in Modified A2/O-BAF].

The effect of hydraulic retention time (HRT) on denitrifying phosphorus and nitrogen removal in a modified two sludge A2/O-BAF system was studied. The influent COD, NH4+-N, and TP were 189.6, 60.4, and 5.1 mg·L-1, respectively. When HRT was 9, 8, 7, and 6 h, the average effluent COD was less than 42 mg·L-1. The average effluent NH4+-N levels were 2.4, 2.8, 3.3, and 6.5 mg·L-1, respectively. The average effluent TP values were 0.3, 0.4, 0.7, and 0.8 mg·L-1, respectively. The ratio of the denitrifying phosphorus accumulation organisms (DPAOs/PAOs) in the system of anoxic zone was reduced from 76.8% to 48.8%. When HRT was 8 h, the ratio of denitrifying phosphorus to nitrogen (ΔPO43-/ΔNO3--N) was increased by 37.5% by a mathematical statistics method. The ΔPO43-/ΔNO3--N in the anoxic zone was 1.24 (the theoretical value is 1.41). At this time, the effect of denitrifying phosphorus to nitrogen was the best. The SVI value was lower than 100 mL·g-1 throughout the experiment, and the MLVSS/MLSS gradually decreased from 0.74 to 0.63, indicating that the sludge activity was reduced.

Genome and Transcriptome Analyses Provide Insight Into the Omega-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis of Schizochytrium limacinum SR21.

Schizochytrium sp. is the best natural resource for omega-3 long-chain polyunsaturated fatty acids. We report a high-quality genome sequence of Schizochytrium limacinum SR21, which has a 63 Mb genome size, with a contig N50 of 2.67 Mb and 6,838 protein-coding genes. Phylogenomic and comparative genomic analyses revealed that DHA-producing Schizochytrium and Aurantiochytrium strains were highly similar and possessed similar genes. Analysis of the fatty acid synthase (FAS) for LC-PUFAs production results in the annotation of all genes in map00062 and map01212. A gene cluster and 10 ORFs related to PKS pathway were found in the genome. 1,402 differentially expressed genes (DEGs) of the treated groups (0.5 g/L yeast extract) were identified by comparing with the control groups (1.0 g/L yeast extract) at 36 h. A weighted gene coexpression network analysis revealed that 2 of 7 modules correlated highly with the fatty acid and DHA contents. The DEGs and transcription factors were significantly correlated with fatty acid biosynthesis, including MYB, Zinc Finger and ACOX. The results showed that these hub genes are regulated by genes involved in fatty acid biosynthesis pathways. The results providing an important reference for further research on promoting fatty acid and DHA accumulation in S. limacinum SR21.

A high activity zinc transporter OsZIP9 mediates zinc uptake in rice.

Zinc (Zn) is an essential micronutrient for most organisms including humans, and Zn deficiency is widespread in human populations, particularly in underdeveloped regions. Cereals such as rice (Oryza sativa) are the major dietary source of Zn for most people. However, the molecular mechanism underlying Zn uptake in rice is still not fully understood. Here, we report that a member of the ZIP (ZRT, IRT-like protein) family, OsZIP9, contributes to Zn uptake in rice. It was expressed in the epidermal and exodermal cells of lateral roots, localized in the plasma membrane and induced during Zn deficiency. Yeast-expressed OsZIP9 showed much higher Zn influx transport activity than other rice ZIP proteins in a wide range of Zn concentrations. OsZIP9 knockout rice plants showed a significant reduction in growth at low Zn concentrations, but could be rescued by a high Zn supply. Compared with the wild type, accumulation of Zn in root, shoot and grain was much lower in knockout lines, particularly with a low supply of Zn under both hydroponic and paddy soil conditions. OsZIP9 also showed Co uptake activity. Natural variation of OsZIP9 expression level is highly associated with Zn content in milled grain among rice varieties in the germplasm collection. Taken together, these results show that OsZIP9 is an important influx transporter responsible for the take up of Zn and Co from external media into root cells.

Diabetes downregulates peptide transporter 1 in the rat jejunum: possible involvement of cholate-induced FXR activation.

Peptide transporter 1 (PepT1), highly expressed on the apical membrane of enterocytes, is involved in energy balance and mediates intestinal absorption of peptidomimetic drugs. In this study, we investigated whether and how diabetes affected the function and expression of intestinal PepT1. Diabetes was induced in rats by combination of high-fat diet and low dose streptozocin injection. Pharmacokinetics study demonstrated that diabetes significantly decreased plasma exposures of cephalexin and acyclovir following oral administration of cephalexin and valacyclovir, respectively. Single-pass intestinal perfusion analysis showed that diabetes remarkably decreased cephalexin absorption, which was associated with decreased expression of intestinal PepT1 protein. We assessed the levels of bile acids in intestine of diabetic rats, and found that diabetic rats exhibited significantly higher levels of chenodeoxycholic acid (CDCA), cholic acid (CA) and glycocholic acid (GCA), and lower levels of lithocholic acid (LCA) and hyodeoxycholic acid (HDCA) than control rats; intestinal deoxycholic acid (DCA) levels were unaltered. In Caco-2 cells, the 6 bile acids remarkably decreased expression of PepT1 protein with CDCA causing the strongest inhibition, whereas TNF-α, LPS and insulin little affected expression of PepT1 protein; short-chain fatty acids induced rather than decreased expression of PepT1 protein. Farnesoid X receptor (FXR) inhibitor glycine-ß-muricholic acid or FXR knockdown reversed the downregulation of PepT1 expression by CDCA and GW4064 (another FXR agonist). In diabetic rats, the expression of intestinal FXR protein was markedly increased. Oral administration of CDCA (90, 180 mg·kg-1·d-1, for 3 weeks) dose-dependently decreased the expression and function of intestinal PepT1 in rats. In conclusion, diabetes impairs the expression and function of intestinal PepT1 partly via CDCA-mediated FXR activation.

A high-quality genome provides insights into the new taxonomic status and genomic characteristics of Cladopus chinensis (Podostemaceae).

The Podostemaceae are ecologically and morphologically unusual aquatic angiosperms that survive only in rivers with pristine hydrology and high water quality and are at a relatively high risk of extinction. The taxonomic status of Podostemaceae has always been controversial. Here, we report the first high-quality genome assembly for Cladopus chinensis of Podostemaceae, obtained by incorporating Hi-C, Illumina and PacBio sequencing. We generated an 827.92 Mb genome with a contig N50 of 1.42 Mb and 27,370 annotated protein-coding genes. The assembled genome size was close to the estimated size, and 659.42 Mb of the assembly was assigned to 29 superscaffolds (scaffold N50 21.22 Mb). A total of 59.20% repetitive sequences were identified, among which long terminal repeats (LTRs) were the most abundant class (28.97% of the genome). Genome evolution analysis suggested that the divergence time of Cladopus chinensis (106 Mya) was earlier than that of Malpighiales (82 Mya) and that this taxon diverged into an independent branch of Podestemales. A recent whole-genome duplication (WGD) event occurred 4.43 million years ago. Comparative genomic analysis revealed that the expansion and contraction of oxidative phosphorylation, photosynthesis and isoflavonoid metabolism genes in Cladopus chinensis are probably related to the genomic characteristics of this growing submerged species. Transcriptome analysis revealed that upregulated genes in the shoot group compared to the root group were enriched in the NAC gene family and transcription factors associated with shoot development and defense responses, including WUSCHEL (WUS), ASYMMETRIC LEAVES (ASL), SHOOT MERISTEMLESS (STM), NAC2, NAC8, NAC29, NAC47, NAC73, NAC83 and NAC102. These findings provide new insights into the genomic diversity of unusual aquatic angiosperms and serve as a valuable reference for the taxonomic status and unusual shoot apical meristem of Podostemaceae.