Review of the Low-Temperature Acoustic Properties of Water, Aqueous Solutions, Lipids, and Soft Biological Tissues.

Existing data on the acoustic properties of low-temperature biological materials is limited and widely dispersed across fields. This makes it difficult to employ this information in the development of ultrasound applications in the medical field, such as cryosurgery and rewarming of cryopreserved tissues. In this review, the low-temperature acoustic properties of biological materials, and the measurement methods used to acquire them were collected from a range of scientific fields. The measurements were reviewed from the acoustic setup to thermal methodologies for samples preparation, temperature monitoring, and system insulation. The collected data contain the longitudinal and shear velocity, and attenuation coefficient of biological soft tissues and biologically relevant substances-water, aqueous solutions, and lipids-in the temperature range down to -50 °C and in the frequency range from 108 kHz to 25 MHz. The multiple reflection method (MRM) was found to be the preferred method for low-temperature samples, with a buffer rod inserted between the transducer and sample to avoid direct contact. Longitudinal velocity changes are observed through the phase transition zone, which is sharp in pure water, and occurs more slowly and at lower temperatures with added solutes. Lipids show longer transition zones with smaller sound velocity changes; with the longitudinal velocity changes observed during phase transition in tissues lying between these two extremes. More general conclusions on the shear velocity and attenuation coefficient at low-temperatures are restricted by the limited data. This review enhance knowledge guiding for further development of ultrasound applications in low-temperature biomedical fields, and may help to increase the precision and standardization of low-temperature acoustic property measurements.

Evaluation of Blood Induced Influence for High-Definition Intravascular Ultrasound (HD-IVUS).

High-definition intravascular ultrasound (HD-IVUS) utilizing more than 80 MHz frequency to assess atherosclerotic plaque, can theoretically achieve an axial resolution of less than [Formula: see text]. However, the blood is a high-attenuation source at high frequency, which would affect the imaging quality. There has been no research evaluating the blood-induced influence on HD-IVUS imaging. And whether a temporary removal of blood is needed for HD-IVUS is unknown. In this study, an ultrahigh-frequency (100 MHz) ultrasound transducer was developed to evaluate the blood-induced attenuation for HD-IVUS imaging. A series of tungsten-wire phantom images in saline and blood at varying hematocrits were obtained. The images showed that blood did influence the ultrahigh-frequency imaging quality greatly. The signal-to-noise ratio (SNR) decrease by 71.7% in porcine whole blood compared to that in saline at the same depth of 2.3 mm. Moreover, the potential flushing schemes for HD-IVUS were studied in varying hematocrits. Three flushing agents commonly used in intravascular optical coherence tomography (IV-OCT) were investigated, including iohexol, mannitol, and dextran 5% and saline as the control group. The attenuation of blood in varying hematocrits/flushing agents was measured from 90 to 110 MHz. The result indicated dextran 5% was a suitable flushing agent for HD-IVUS due to its less signal attenuation compared to others.

On-Chip Multicolor Photoacoustic Imaging Flow Cytometry.

On-chip imaging flow cytometry has been widely used in cancer biology, immunology, microbiology, and drug discovery. Pure optical imaging combined with flow cytometry to derive chemical, structural, and morphological features of cells provides systematic insights into biological processes. However, due to the high concentration and strong optical attenuation of red blood cells, preprocessing is necessary for optical flow cytometry while dealing with whole blood. In this study, we develop an on-chip photoacoustic imaging flow cytometry (PAIFC), which combines multicolor high-speed photoacoustic microscopy and microfluidics for cell imaging. The device employs a micro-optical scanner to achieve a miniaturized outer size of 30 × 17 × 24 mm3 and ultrafast cross-sectional imaging at a frame rate of 1758 Hz and provides lateral and axial resolutions of 2.2 and 33 µm, respectively. Using a multicolor strategy, PAIFC is able to differentiate cells labeled by external contrast agents, detect melanoma cells with an endogenous contrast in whole blood, and image melanoma cells in blood samples from tumor-bearing mice. The results suggest that PAIFC has sufficient sensitivity and specificity for future cell-on-chip applications.

Flexible Pico-Liter Acoustic Droplet Ejection Based on High-Frequency Ultrasound Transducer.

Acoustic droplet ejection (ADE) uses the acoustic energy produced by a focused ultrasound beam to provide a noncontact, highly precise, automatic, and cost-effective liquid transfer method for life science applications. The reported minimum precision of the current acoustic liquid transfer technology is 1 nL. Since precision improvement always brings valuable results in biological research, it is highly necessary to develop pico-liter precision liquid transfer technology. In this work, we developed a 40-MHz ultrahigh -frequency focused ultrasound transducer with a large aperture of 7×7 mm2 and a wide bandwidth of 76.4%. The designed transducer can successfully eject pico-liter droplets, and the droplet ejection accuracy ranges from 28 to 439 pL. The effects of the acoustic parameters, including excitation amplitude, pulsewidth, and frequency, on the size of the ejected droplet were studied. A wide range of ejected droplet sizes could be obtained by adjusting the acoustic parameters, thereby making liquid transfer flexible. The flexible pico-liter liquid transfer based on the wide-bandwidth, high-frequency ultrasound transducer is easier to achieve automatically, and thus it has broad prospects in biological research and industrial applications.

Cable shared dual-frequency catheter for intravascular ultrasound.

This study proposes a catheter consisting of dual-frequency transducer for intravascular ultrasound. Both ultrasonic elements with different frequencies were connected to one coaxial cable to make the connection simple. The aperture size of the ultrasound elements were 0.4×0.6 mm2 and 0.3×0.4 mm2 for the low frequency element and high frequency element, respectively. The center frequency and bandwidth of the fabricated low frequency transducer were 33.8 MHz and 49.3%, respectively. Meanwhile, the center frequency and bandwidth of the high frequency transducer were 80.6 MHz and 50.3%, respectively. Imaging evaluations of wire phantom, tissue phantom and vessel tissue demonstrated good imaging capability of the dual-frequency catheter. The spatial resolution are 19 µm axially and 128 µm laterally for the high frequency transducer, and 37 µm axially and 199 µm laterally for the low frequency transducer. Band-pass filters were designed to separate the mixed echo signals. After filtering, the images from different ultrasound elements can be successfully identified, indicating the feasibility of the proposed cable shared dual-frequency imaging strategy. The proposed method has simple structure, good imaging resolution, and large penetration depth, showing good application prospect for intravascular ultrasound.