Multi-directional differentiation renal cell cancer: a case report / 中华泌尿外科杂志

Renal cancer with multiple differentiation and multiple components is rare in clinic. It has the character of high malignancy, early metastasis, and poor prognosis. A patient with multiple differentiated renal cell carcinoma was diagnosed in our hospital mainly because of emaciation, fatigue and loss of appetite. CT scan showed the 6.5 cm×6.0 cm mass in the lower pole of right kidney with unequal enhancement and necrosis. The chest CT scan showed multiple enlarged lymph nodes in the mediastinum and right superior clavicle. The patient accepted the laparoscopic retroperitoneal right nephrectomy and right supraclavicular lymph node biopsy. Postoperative pathology showed multiple differentiated renal cell carcinoma with localized sarcomatoid leisions, including clear cell carcinoma, collecting duct-like carcinoma and medullary carcinoma combined with right supraclavicular lymph node metastasis. Targeted therapy was performed at 1 month after surgery. Liver and lung metastasis occured at 6 months and the patient died of multi-organ failure at 12 months after surgery.

The practice of and reflection on hospital publicity in post-epidemic era / 西安交通大学学报(医学版)

In the wake of the COVID-19 pandemic, only through whole-hearted cooperation and joined efforts can medical institutions, governments, and social organizations cope with emergency, convert the crisis into opportunities, promote modern governance and accomplish high-quality development in the fight against the pandemic. Taking the First Affiliated Hospital of Xi’an Jiaotong University as an example, this paper analyzes the all-media communication matrix of "one network platform, 3W + N" that the hospital established during the pandemic by combining the authoritative official website with WeChat, Wesee, Weibo and that other publicity media. The study explores how to create a publicity brand that is mindful, poignant, compassionate and devoted and maintains its scientific and refined management, so as to achieve the professional goal of educating the public, shouldering the responsibility in the combat against the pandemic, and taking the lead in the research on the pandemic.

Expression of MCM6 in bladder cancer and its correlation with prognosis / 国际生物医学工程杂志

Objective:To detect the expression of microchromosome maintenance protein 6 (MCM6) in bladder cancer tissues, and to explore the relationship between MCM6 and the clinical prognosis of bladder cancer. To explore the possibility of MCM6 as a potential biomarker for bladder cancer.Methods:Bioinformatics methods were used to analyze the mRNA levels of MCM6 in bladder cancer tissues and adjacent normal tissues, and to analyze the relationship between its expression and the survival rate of bladder cancer patients. The clinicopathological data of 83 patients with bladder cancer underwent surgical treatment were retrospectively analyzed. Immunohistochemical method was used to detect the expression level of MCM6 protein in bladder cancer tissue and normal tissue adjacent to the cancer, and analyze its relationship with clinicopathological characteristics of bladder cancer patients.Results:The results of bioinformatics analysis showed that MCM6 mRNA was significantly highly expressed in bladder cancer tissues and was significantly correlated with the overall survival rate ( P=0.036) and disease-free survival rate ( P=0.01) of the patients. The results of immunohistochemistry showed that the expression of MCM6 in bladder cancer tissue was significantly higher than that in normal tissues adjacent to the cancer, and high expression of MCM6 in bladder cancer tissue was related to tumor stage ( P=0.025), but had nothing to do with age, gender and tumor grade (all P>0.05). Conclusions:The expression of MCM6 in bladder cancer tissue is significantly high and indicates a poor prognosis, and its expression level is related to the tumor stage of bladder cancer patients. MCM6 may serve as a new potential biomarker for bladder cancer.

Expression level of ZNF217 in bladder cancer and its clinical significance / 国际生物医学工程杂志

Objective To determine the expression of ZNF217 protein in bladder cancer tissues and to analyze its clinicopathological characteristics, so as to clarify its clinical significance. Methods The clinicopathological data of 123 patients with bladder cancer who underwent bladder cancer resection were retrospectively analyzed. The expression of ZNF217 protein in bladder cancer tissues and corresponding adjacent tissues was detected by immunohistochemistry, and its correlation with clinical characteristics of patients was analyzed. Results ZNF217 protein was highly expressed in tumor tissues, and the ratio of high expression of tumor tissues was 67.4％, but it was lowly expressed in adjacent tissues. By comparing with clinicopathological features, the expression level of ZNF217 was significantly correlated with tumor T stage and tumor recurrence (all P<0.05), but not correlated with age , gender , tumor differentiation and lymph node metastasis (all P>0.05). The survival analysis showed that patients with high expression of ZNF217 in bladder cancer had significantly shorter overall survival and progression-free survival than those with low ZNF217 expression ( all P<0 . 05 ) . Conclusions ZNF217 is highly expressed in bladder cancer tissues, and its expression level is associated with tumor stage, tumor recurrence and poor prognosis, suggesting that it can be used as a potential therapeutic target for bladder cancer.

Effect of High Positive Acceleration (+Gz) Environment on Dental Implant Osseointegration:A Preliminary Animal Study / 生物医学与环境科学（英文）

OBJECTIVE@#To observe the effect of high positive acceleration (+Gz) environment on dental implant osseointegration in a rabbit model and to investigate its mechanism.@*METHODS@#Forty-eight New Zealand white rabbits were randomly divided into 6 groups. The rabbit's mandibular incisors were extracted and 1 implant was placed in each socket immediately. After 1 week of rest, the rabbits were exposed to a high +Gz environment, 3 times a week. The rabbits were sacrificed at 3 weeks (2 weeks +Gz exposure), 5 weeks (4 weeks +Gz exposure), and 12 weeks (4 weeks +Gz exposure and 7 weeks normal environment) after surgery, respectively. Specimens were harvested for micro-CT scanning, histological analysis, and real-time polymerase chain reaction examination.@*RESULTS@#Compared with those in the control group, the mRNA expression levels of bone morphogenetic protein-2 (BMP-2), osteopontin (OPN), and transforming growth factor-β1 (TGF-β1) were significantly lower (P < 0.05), while the mRNA expression level of receptor activator of nuclear factor κB ligand (RANKL) and the RANKL/osteoprotegerin (OPG) ratio were significantly higher (P < 0.05) at 3 weeks; values of bone volume fraction, trabecular number, bone-implant contact (BIC), and TGF-β1 and OPG mRNA expression levels were significantly lower (P < 0.05), and the value of trabecular separation, RANKL mRNA expression level and RANKL/OPG ratio were significantly higher (P < 0.05) at 5 weeks; and the value of BIC was still significantly lower (P < 0.05) at 12 weeks in the experimental group.@*CONCLUSION@#Early exposure to the high +Gz environment after implant surgery might have an adverse effect on osseointegration, and its mechanism could be related to the inhibition of osteoblast activity and promotion of osteoclast activity.

Expression of Notch4 in renal cell carcinoma and its relationship with the microvascular density / 实用医学杂志

Objective To investigate the expression of Notch4 protein and to analyze its correlation with the clinical parameters and the microvessel dentisty in renal cell carcinoma. Methods The expression of Notch4 was examined in 60 cases of renal cell carcinoma and the para-carcinoma tissue by SP immunohistochemical stain-ing ,and CD34 detection was used for counting microvessel density. Statistical analysis was performed to reveal the correlation with clinicopathological parameters ,microvessel density and prognosis. Results The positive rate of Notch4 protein expression was 75%(45/60)in para-carcinoma tissue,and was 43.3%(26/60)in renal cell car-cinoma,with significant difference on tumor grade and Lymph node metastasis(P<0.05). The microvessel densi-ty in Notch4 positive tissues was significant lower than that in the negative samples(P<0.05). The survival time of patients with Notch4 positive expression was significantly longer than that of patients with Notch4 negative expres-sion(P<0.05). Conclusion Notch4 protein plays an important role in the development of renal cell carcinoma. Notch4 expression might both attenuate the malignant biological characteristics and suppress the angiogenesis dur-ing tumor development.

Impact of different extents of lymph node dissection on the survival in stage III esophageal cancer patients / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the safety, feasibility and the impact of different extents of lymph node dissection on the survival in the patients with locally advanced thoracic esophageal carcinoma.</p><p><b>METHODS</b>From January 2001 to December 2006, 122 patients with locally advanced thoracic esophageal carcinoma underwent radical resection through cervical, thoracic, and abdominal incisions, and were randomly divided into two-field lymph node dissection group (Two-FD) and three-field lymph node dissection group (Three-FD). Life-table method was used to compare the difference of survival rates between the two groups. Kaplan-Meier method was used to compare the cumulative survival time and median survival time between the two groups. Multivariate analysis was performed using Cox model to identify the prognostic factors affecting the survival (alpha = 0.05).</p><p><b>RESULTS</b>There was no significant difference between the two groups in age, sex, and disease stage. Postoperative complication rate and perioperative mortality rate were 14.5% and 1.6% in the two-FD group versus 15.0% and 1.7% in the three-FD group, statistically without a significant difference (P > 0.05). The 1-, 3- and 5-year survival rates were 78.2%, 39.6% and 14.5% in the two-FD group, and 83.7%, 42.4% and 18.1% in the three-FD group, respectively. The median survival time was 24.0 months in the two-FD group and 31.0 months in the three-FD group. Log-rank analysis showed that in the patients without preoperative weight loss, in T3N1M0 stage, only single regional lymph node metastasis but < 3 in total, the three field lymph node dissection achieved a better prognosis (P < 0.05). Multivariate analysis using Cox model showed that T and N stages and lymph node dissection extent were still risk factors in patients with stage III locally advanced thoracic esophageal carcinoma.</p><p><b>CONCLUSION</b>Compared with the two field lymph node dissection, the three field lymph node dissection is safe and feasible, and can improve the survival for a part of stage III esophageal cancer patients without increase in operative mortality and complications.</p>

Comparison and analysis of human dentin matrix protein 1 promoter activity in three different cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe and compare the luciferase activities of different length segments of human dentin matrix protein 1 promoter in human dental pulp stem cells (HDPSC), osteoblasts (OC) and Hela cells.</p><p><b>METHODS</b>The differentlength desired DNA segments were obtained from 2 195 bp Dmp1 promoter cloned by PCR method. The amplified promoter segments with different length were cloned into luciferase report gene vector pGL3-Basic, the correct orientation of those inserts was verified by cutting with two different restrict enzymes. The luciferase activity was observed after different pGL3-PDmp1 vectors were transfected transiently into those three different-type cells.</p><p><b>RESULTS</b>6 Dmp1 promoter segments with different-length were obtained successfully, and luciferase report gene vectors with different promoter segments were successfully constructed after identified by restriction enzymes cutting. They had different luciferase activities when they were transfected transiently into HDPSC, and the region of -505(-)-193 bp and -935(-)-505 bp could be regarded as the specific promoters of Dmp1 promoter for HDPSC and OC respectively, which could include the basic regulatory elements.</p><p><b>CONCLUSION</b>The correct clone of the upstream of human Dmp1 promoter segments with different length had been obtained, and they had strong luciferase activities in HDPSC and OC, but very low in Hela cell. These results will make an important basis for studying mineralized tissue-specific transcriptional regulation mechanisms of Dmp1.</p>

Synthesis and structural characterization of a novel mixed-valent Cu(II)Cu(I)Cu(II) triangular metallomacrocycle using an imine-based rigid ligand.

A novel neutral mixed-valent Cu(I)Cu(II)(2) triangular metallomacrocycle [Cu(3)L(2)(HL)].3CH(3)OH.2H(2)O (1) was assembled by reaction of the tetradentate ligand bis(N-salicylidene-4,4'-diphenylamine), H(2)L, with a copper(II) salt. ESI-MS show peaks only corresponding to the triangular structural species, indicating the high stability of the trimer structure in solution. Magnetic study confirms that there are two Cu(II) ions and one Cu(I) ion in a discrete triangular molecule. The crystal structure of 1 reveals that the triangle is formed by three deprotonated ligands and three copper ions with a Cu-Cu separation of ca. 11.8 A. Each copper atom is coordinated by two oxygen atoms and two nitrogen atoms from two different bis-bidentate ligands in a heavily distorted tetrahedral geometry, while each ligand is bound to two metal ions in a bis-bidentate coordination mode and links the metal centers overlapping in an unprogressive manner. Strong intramolecular pi.pi interactions between the ligands are found to stabilize the constraint conformation of the triangle. Electrochemical study reveals that the mixed-valent Cu(I)Cu(II)(2) complex is the most stable state in solution condition, and the electrochemical communication between the copper ions might be explained on the basis of the through-bond interaction. UV-vis-NIR spectral measurement demonstrates the Robin-Day class II behavior of the mixed-valence compound with a weak copper-copper interaction.

Design and crystal structures of triple helicates with crystallographic idealized D3 symmetry: the role of side chain effect on crystal packing.

Novel crystallographic D3-symmetric binuclear triple molecular helices [Co2L(1)3][BF4]4 (1), [Zn2L(1)3][BF4]4 (2), [Mn2L(1)3][BF4]4 (3), [Co2L(2)3][BF4]4 (4), [Zn2L(2)3][BF4]4 (5), and [Mn2L(2)3][BF4]4 (6) have been achieved to establish the side chain effect on molecular packing, where L1 is [(C5H4N)C(CH3)=N-(C6H4)-]2CH2 and L2 is [(C5H4N)C(CH3)=N-(C6H4)-]2O, respectively. Crystal structure analyses show that each helix crystallizes in a hexagonal crystal system with space group Pc1 and the general axis of the helix occupies the crystallographic 3-fold axial position with the other three crystallographic 2-fold symmetries perpendicular to it. Each metal center is bound to three pyridylimine units to attain C3 pseudooctahedral coordination geometry with respective equivalent metal-N (CH=N) and metal-N (pyridyl) bonds. It is speculated that the existence of the methyl group might minimize the potential intermolecular interactions, which would be the essential factor controlling the helices formed in idealized crystallographic D3 symmetry. Moreover, crystallographic idealized C3-symmetric helicates [Co2L(3)3][BF4]4 (7), [Zn2L(3)3][BF4]4 (8), [Ni2L(3)3][BF4]4 (9), and [Cu2L(3)3][BF4]4 (10) were also structurally characterized for comparison, where L3 is [(C5H4N)C(CH3)=N-]2. All the results indicate that the existence of the methyl group in the side chain of aromatic ligands could effectively reduce the potential - intermolecular interactions and the side chain effect of the methyl group in crystal packing is robust enough to be exchanged from one network structure to another, which ensures the generality and predictability of the crystallographic idealized symmetry formation to a certain extent.

Novel ferrocene-containing helical triangular macrocycle achieved via an exchange reaction.

A novel ferrocene-containing helical triangular macrocyclic compound has been constructed through an exchange reaction of hydrazone groups which templated and catalyzed by Co(II) ions.