Meta-Analysis on the Anesthetic Effects of Remifentanil plus Dexmedetomidine versus Remifentanil Alone in Cardiac Surgery.

In this study, we performed a meta-analysis to investigate the anesthesia effects of remifentanil plus dexmedetomidine versus remifentanil alone in cardiac surgery. Literature search was performed on PubMed, Web of Science, Embase, China Knowledge Infrastructure, Wanfang Data, and other databases for relevant literature published in English or Chinese before October 2021. A total of 17 studies, consisting of 1350 patients, were included in this study. Of these, 10 studies showed that remifentanil plus dexmedetomidine had a good anesthesia effect in cardiac surgery (OR = 3.61, 95% CI: 1.73, 7.52, P < 0.001), and 8 studies showed that the Ramsay score test of anesthesia (SMD = 0.88; 95% CI: -0.77, 2.53; P < 0.001) in the experimental group was better than that in the control group. In addition, changes in the hemodynamic heart rate (SMD = -0.74; 95% CI: -1.41, -0.07; P < 0.001) and mean arterial pressure (SMD = -0.18; 95% CI: -0.72, 0.36; P < 0.001) of the two groups of anesthesia were counted in 17 studies, which also showed that the anesthesia effect of remifentanil plus dexmedetomidine was good. Thus, remifentanil plus dexmedetomidine may be a more promising option for cardiac surgery anesthesia than remifentanil alone.

[Expression of endoglucanase gene in Bacillus Megaterium and characterization of recombinant enzyme].

The signal peptide-encoding sequence, which was included in the gene (GenBank No. DQ782954) encoding for endoglucanase, was removed by PCR. The gene without the signal peptide was then ligated with the expression plasmid pHIS1525. The recombination plasmid pHIS1525 was transformed into Escherichia coli DH5a and the transformant was designated DH5a-pHIS1525-G7. The plasmid from the recombinant DH5a-pHIS1525-G7 was transformed into the proto-plasts of Bacillus megaterium strains WH320, and the genetically engineered bacterium, known as WH320-pHIS1525-G7, was acquired. The effective expression of the gene in the recombinant was confirmed by Congo-red dyeing and SDS poly-acrylamide gel electrophoresis (SDS-PAGE). WH320-pHIS1525-G7 was cultured in optimum condition. The activity of the endoglucanase was 899U, which was 11.22-fold higher than that of B.subtilis C-36. The properties of enzyme were deter-mined. The optimum temperature and pH value were 65 and pH 6.0, respectively. The enzyme maintained over 80% of the original enzyme activity between pH 4.5 and pH 10.0 after incubated at 50 for 30 min.