Assessment of environmental exposure to betamethasone on the reproductive function of female Japanese medaka (Oryzias latipes).

Betamethasone has been extensively used in medicine in recent years and poses potential hazards to aquatic organisms. This study investigated the reproductive toxic effects of betamethasone exposure in fish, employing female Japanese medaka (Oryzias latipes) as a model. Betamethasone exposure at environmentally relevant concentrations (0, 20, 200, and 2000 ng/L) for a period of 15 weeks resulted in its high accumulation in the ovary, leading to abnormal oogenesis in female Japanese medaka. The production of gonadotropins (LH and FSH) in the pituitary gland was inhibited, and sex steroid biosynthesis in the ovary was significantly influenced at the transcriptional level. The imbalance of androgens and estrogens resulted in a decrease in the E2/T ratio and hepatic VTG synthesis, and the suppression of estrogen receptor signaling was also induced. Furthermore, betamethasone exposure delayed spawning and reduced fertility in the F0 generation, and had detrimental effects on the fertilization rate and hatchability of the F1 generation. Our results showed that environmental betamethasone had the potential to adversely affect female fertility and steroid hormone dynamics in fish.

Long-term hypoxia stress-induced oxidative stress, cell apoptosis, and immune response in the intestine of Pelteobagrus vachelli.

Hypoxia is a common phenomenon in aquaculture. With the dissolved oxygen (DO) 3.75 ± 0.25 mg O2 /L for hypoxia group and 7.25 ± 0.25 mg O2 /L for control group for 30, 60, and 90 days, long-term hypoxia stress was used to investigate the oxidative stress, apoptosis, and immunity in the intestine of Pelteobagrus vachelli. According to the results of measurement of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) activities and malondialdehyde (MDA) content, the oxidative stress ability of the intestine was activated at 30 days and impaired at 60 and 90 days. The upregulation of Bcl-2-associated x (Bax); downregulation of B cell lymphoma-2 (Bcl-2); increased activities of caspase-3, caspase-9, and Na+-K+-ATPase; decreased activities of succinate dehydrogenase (SDH); and the release of cytochrome c (Cyt-c) in mitochondria revealed that hypoxia induced the apoptosis. Moreover, heat shock protein 70 (HSP 70), heat shock protein 90 (HSP 90), immunoglobulin M (IgM), and C-lysozyme (C-LZM) were activated to inhibit apoptosis, but the immunoregulatory function might be damaged at 60 and 90 days. This study provides a theoretical foundation for understanding the mechanisms of hypoxia stress and aquaculture management of P. vachelli.

iTRAQ-based quantitative proteomic analysis of Pelteobagrus vachelli liver in response to hypoxia.

Dissolved oxygen is one of the determinants in the healthy farming of Pelteobagrus vachelli. This study, we conducted quantitative proteomics on the juvenile P. vachelli livers using iTRAQ. P. vachelli were treated by 3.75 ± 0.25 mg O2/L (hypoxia group) and 7.25 ± 0.25 mg O2/L (control group) for 90 days. The results revealed that under hypoxic conditions, P. vachelli grew slower than control group. Proteomic profiling enabled us to identify 2618 proteins, of which 176 were significantly differentially abundant proteins (DAPs). Verification of protein regulation based on qRT-PCR indicated that the proteomics data were reliable. The top 20 significantly DAPs (10 up-regulated, 10 down-regulated) were primarily involved in energy metabolism, apoptosis inhibition, and heavy metal detoxification. KEGG pathway enrichment analysis revealed significant enrichment of 'protein digestion and absorption', 'glycolysis/gluconeogenesis', and 'phagosome'. Combining the proteomics results of short-term hypoxia (treated with 0.70 ± 0.10 mg O2 /L for 4 h), we screened 36 common DAPs. The analysis of the 36 common DAPs indicated that P. vachelli responded to the hypoxia by regulating energy supply, inhibiting apoptosis, and disturbing defensive system. Our results lay a theoretical foundation for the cultivation of hypoxia-tolerant species and eco-breeding of P. vachelli. SIGNIFICANCE OF THE STUDY: The hypoxia tolerance of Pelteobagrus vachelli is poor, which will seriously lead to its death in high-density culture. This study analysed the liver proteome of P. vachelli under long-term hypoxia stress (treated for 90 days at 3.75 ± 0.25 mg O2/L), and then combined the proteome results of short-term hypoxia stress (treated for 4 h at 0.70 ± 0.10 mg O2/L). The results showed P. vachelli responded to the hypoxia by regulating energy supply, inhibiting apoptosis and disturbing defensive system. The study contributes to the breeding of new hypoxia-tolerant species of P. vachelli and lays the theoretical foundation for eco-breeding.