Assuntos
Antígenos de Neoplasias/análise , Antígenos Nucleares/análise , Linfoma/química , Proteínas Nucleares/análise , Anticorpos/uso terapêutico , Antígenos de Neoplasias/imunologia , Antígenos Nucleares/imunologia , Linhagem Celular Tumoral , Humanos , Linfoma/terapia , Proteínas Nucleares/imunologiaRESUMO
It has been proposed that a family of small soluble binding proteins, variously named as chemosensory proteins, sensory appendage proteins, pherokines and OS-D-like proteins, are involved in insect chemoreception. These proteins are present in a wide range of insect species and have a characteristic four cysteine motif. We have cloned cDNAs and gene sequences encoding these proteins from a number of aphid species, the first report of such in the order Hemiptera and supporting the view that these genes predate the divergence of the Neoptera. In one aphid species, Megoura viciae, we have shown that the proteins are present primarily in adults with one protein being most abundant in antennae and legs. This supports the view that the proteins could be involved in chemoreception but our preliminary binding studies failed to detect binding to a range of compounds which are known to elicit an electrophysiological response by aphids.
Assuntos
Afídeos/genética , Proteínas de Insetos/biossíntese , Sequência de Aminoácidos , Animais , Afídeos/metabolismo , Sequência de Bases , Ligação Competitiva , Western Blotting , Clonagem Molecular , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Insetos/genética , Dados de Sequência Molecular , Ligação Proteica , RNA/química , RNA/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de SequênciaRESUMO
Here we report that the OX-TES-1 SEREX antigen, which showed immunological reactivity with serum from four out of 10 diffuse large B-cell lymphoma (DLBCL) patients, is encoded by a novel gene, PAS domain containing 1 (PASD1). PASD1_v1 cDNA encodes a 639 amino-acid (aa) protein product, while an alternatively spliced variant (PASD1_v2), lacking intron 14, encodes a 773 aa protein, the first 638 aa of which are common to both proteins. The PASD1-predicted protein contains a PAS domain that, together with a putative leucine zipper and nuclear localisation signal, suggests it encodes a transcription factor. The expression of PASD1_v1 mRNA was confirmed by RT-PCR in seven DLBCL-derived cell lines, while PASD1_v2 mRNA appears to be preferentially expressed in cell lines derived from non-germinal centre DLBCL. Immunophenotyping studies of de novo DLBCL patients' tumours with antibodies to CD10, BCL-6 and MUM1 indicated that two patients mounting an immune response to PASD1 were of a poor prognosis non-germinal centre subtype. Expression of PASD1 mRNA was restricted to normal testis, while frequent expression was observed in solid tumours (25 out of 68), thus fulfilling the criteria for a novel cancer testis antigen. PASD1 has potential for lymphoma vaccine development that may also be widely applicable to other tumour types.
