[Effects of prostacyclin MM-706 on liver function restoration in dogs after severe hemorrhagic shock]. / Vplyv prostatsyklinu MM-706 na vidnovlennia funktsii pechinky u sobak pislia tiazhkoho hemorahichnoho shoku.

The new synthetic analogue of prostaglandin I2 (prostacyclin MM-706) influence on some hepatic function indexes was studied up in 7 dogs with an acute hepatic insufficiency caused by severe hemorrhagic shock. Rapid and steady normalization of indexes and the animals survival were promoted by intravenous infusion of MM-706 given in 300 ng/kg dose together with hemocorrector named lactoprotein given in 10 ml/kg dose (7,5 +/- 1,4) hours later. Possible action mechanisms of the MM-706 therapeutic efficacy are considered. Results of investigation performed constitute an experimental base of the MM-706 application in the complex treatment of an acute hepatic insufficiency of different genesis in the clinic.

[The effect of prostacyclin and its synthetic analog MM-706 on kidney function in corrosive sublimate-induced nephropathy]. / Vliianie prostatsiklina i ego sinteticheskogo analoga MM-706 na funktsional'noe sostoianie pochek pri sulemovoi nefropatii.

The effects of prostacyclin and its stable analogue MM-706 on renal function were examined in rats with sublimate nephropathy. Prostacyclin was shown to attenuate renal dysfunctions by increasing glomerular filtration rate and water and salt reabsorption. MM-706 produced more pronounced protective effects and restored the filtration and reabsorption up to the level observed in control rats.

[Effect of a synthetic prostacyclin analog on kidney function and the system of regulation of blood coagulation in experimental acute renal failure]. / Vliianie sinteticheskogo analoga prostatsiklina na funktsiiu pochek i sistemu reguliatsii agregatnogo sostoianiia krovi pri éksperimental'noi ostroi pochechnoi nedostatochnosti.

The influence of a stable analogue of prostacyclin on the renal function and parameters of hemostasis was investigated in experiments on animals with the nephrotic form of acute renal insufficiency. Diuresis, glomerular filtration, water and sodium reabsorption in the presence of diminished intravascular coagulation were recovered after two injections of the compound in a dose of 115 micrograms/kg.

[Action of carbacyclic 13,14-didehydro-analogs of prostacyclin on the contractile activity of the rat stomach muscles]. / Deistvie karbitsiklicheskikh 13,14-didegidro-ananlogov prostatsiklina na sokratitel'nuiu aktivnost' myshtsy zheludka krysy.

The pharmacological activity of bicyclooctane and bicycloheptane analogues of prostacyclin was studied on the rat stomach muscle strip. All the analogues are partial agonists. EC50 of the most active of them, 5E isomer of bicyclooctane analogue, is 2.8.10(-7) M-1.2.10(-7) M. 5E isomers of both bicyclooctane and bicycloheptane analogues with a normal length of alpha-chain are at least by an order of values more active than the corresponding 5Z isomers. At the increase of alpha-chain by one methylene group the activity of 5E and 5Z isomers of bicycloheptane analogue becomes similar.

[Interactions of thyrotropin and prostaglandins E2 with receptors and their effect on the cAMP levels in human thyroid tissue]. / Vzaimodeistvie tireotropnogo gormona i prostaglandinov E2 s retseptorami i ikh vliianie na uroven' tsAMF v tkani shchitovidnoi zhelezy cheloveka.

A study was made of the interactions of prostaglandins E2 (PGE2) and TTH with receptors and their effect on the cAMP level using thyroid tissue obtained suboperatively from patients with nodular euthyroid goiter and diffuse toxic goiter. There was no significant difference in the kinetic characteristics of TTH-receptor interaction in euthyroid and thyrotoxic thyroid tissue. At the same time in thyrotoxicosis there was a decrease in the pool of free receptors to 3H-PGE2, probably, determined by a rise of the formation of receptor complexes with endogenous PGE2 whose content in thyrotoxic tissue was raised. In thyrotoxic thyroid tissue the PGE2 effect maintained in the presence of a decrease in the stimulating TTH action on the cAMP level.

[Characteristics of prostaglandin E1 interaction with components of biological membranes]. / Osobennosti vzaimodeistviia prostaglandina E1 s komponentami biologicheskikh membran.

To estimate the connection between physico-chemical characteristics and biological activity of prostaglandins the interaction of prostaglandin E1 with biological membrane lipids was studied. It is shown that as a result of prostaglandin interaction with phosphatidylcholine a complex is formed that behaves as an individual component and occupies in the surface layer twice as large area than the complex with prostaglandin F2 alpha. The prostaglandin E1 film collapses earlier than F2 alpha. Both facts indicate that the first is more friable. A difference in morphology of prostaglandin monolayers was revealed by electron microscopy. When studying the catalytic activity of peroxidase incorporated in prostaglandin E1 and F2 alpha monolayers some differences were also revealed. In the second case oxidation with methylblue located under the monolayer proceeds more actively. The results obtained point to the connection between the regulatory function of prostaglandins and their chemical structure. Molecular rearrangements of the monolayer caused by prostaglandin incorporation were recorded.

[Interaction of prostaglandin E2 with receptors and their effect on adenylate cyclase activity in human thyroid tissue]. / Vzaimodeistvie prostaglandinov E2 s retseptorami i ikh vliianie na aktivnost' adenilattsiklazy v tkani shchitovidnoi zhelezy cheloveka.

The investigations have been performed on thyroid tissue excised from patients suffering from euthyroid goiter and thyrotoxicosis. The study of thyroid thyrotoxic tissue has revealed the decrease in the number of free receptors to 3H-PGE2 caused by the enhanced production of receptor-endogenous PGE2 complexes whose level in thyrotoxic tissue was significantly higher than in euthyroid tissue. PGE2 and GppNHp had a pronounced stimulating effect on adenylate cyclaze activity both in euthyroid and thyrotoxic tissues, with enzyme sensitivity to stimulating effects considerably increased in the latter case.