RESUMO
Oysters (Oys) contain various beneficial components, such as, antioxidants and amino acids. However, the effects of Oys or taurine (Tau), a major amino acid in Oys on bone growth have not been determined. In the present study, we evaluated the effects of Oys or Tau on linear bone growth in a mouse model of protein malnutrition. To make the protein malnutrition in a mouse, we used a low protein diet. Growth plate thickness was increased by Oys or Tau. Bone volume/tissue volume, trabecular thickness, trabecular number, connection density, and total porosity were also improved by Oys or Tau. Oys or Tau increased insulin-like growth factor-1 (IGF-1) levels in serum, liver, and tibia-growth plate. Phosphorylations of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5) were increased by Oys and by Tau. These findings show that Oys or Tau may increase growth plate thickness by elevating IGF-1 levels and by promoting the phosphorylations of JAK2-STAT5, and suggest that Oys or Tau are growth-promoting substances of potential use in the food and pharmaceutical industries.
Assuntos
Desenvolvimento Ósseo/efeitos dos fármacos , Osso e Ossos/efeitos dos fármacos , Dieta com Restrição de Proteínas/efeitos adversos , Suplementos Nutricionais , Desnutrição/dietoterapia , Taurina/administração & dosagem , Animais , Densidade Óssea/efeitos dos fármacos , Densidade Óssea/genética , Desenvolvimento Ósseo/genética , Osso e Ossos/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica , Fator de Crescimento Insulin-Like I/agonistas , Fator de Crescimento Insulin-Like I/genética , Fator de Crescimento Insulin-Like I/metabolismo , Janus Quinase 2/genética , Janus Quinase 2/metabolismo , Masculino , Desnutrição/etiologia , Desnutrição/genética , Desnutrição/patologia , Camundongos , Camundongos Endogâmicos ICR , Ostreidae/química , Fosforilação , Porosidade/efeitos dos fármacos , Fator de Transcrição STAT5/agonistas , Fator de Transcrição STAT5/genética , Fator de Transcrição STAT5/metabolismo , Transdução de SinaisRESUMO
Given that tea contains a number of chemical constituents possessing medicinal and pharmacological properties, green tea seed is also believed to contain many biologically active compounds such as saponin, flavonoids, vitamins, and oil materials. However, little is known about the physiologic functions of green tea seed oil. The aim of this study is to investigate the anti-obesity effects of green tea seed oil in C57BL/6J mice and in preadipocyte 3T3L-1 cell lines. In vivo, three groups of mice were fed with a standard diet, a high-fat diet containing 30% shortening, or 30% of green tea seed oil based on a standard diet for 85 days. The levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglyceride, glucose, and alanine aminotransferase in blood were analyzed at the end of the study. The mice given green tea seed oil gained less weight compared to mice given the shortening diet (p < 0.01). The plasma level of total cholesterol was decreased by a significant level of 32.4% in mice given the green tea seed oil compared to the mice given the shortening diet (p < 0.01). In addition, 3T3-L1 cells were treated for 2 days to evaluate effects of green tea seed oil on adipocyte differentiation. Green tea seed oil inhibited expression of peroxisome proliferator-activated receptor-gamma(2) and CCAAT/enhancer binding protein-alpha in adipocytes and adipose tissue from the experimental animals. These results indicate that the anti-obesity effects of green tea seed oil might be, in part, through suppression of transcription factors related to adipocyte differentiation.
