Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Intervalo de ano de publicação
1.
Rev Soc Bras Med Trop ; 50(1): 99-103, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28327809

RESUMO

INTRODUCTION:: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2). METHODS:: HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified. RESULTS:: Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells. CONCLUSIONS:: This study provides insights into the mechanisms underlying immune evasion by DV.


Assuntos
Vírus da Dengue/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Vírus da Dengue/classificação , Regulação da Expressão Gênica , Células Hep G2 , Humanos , Subunidades Proteicas , Sorogrupo
2.
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;50(1): 99-103, Jan.-Feb. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-1041391

RESUMO

Abstract: INTRODUCTION: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2). METHODS: HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified. RESULTS: Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells. CONCLUSIONS: This study provides insights into the mechanisms underlying immune evasion by DV.


Assuntos
Humanos , Vírus da Dengue/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Regulação da Expressão Gênica , Subunidades Proteicas , Vírus da Dengue/classificação , Células Hep G2 , Sorogrupo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA