TH17 cells, interleukin-17 and interferon-γ in patients and households contacts of leprosy with multibacillary and paucibacillary forms before and after the start of chemotherapy treatment.

BACKGROUND: Studies on the immunology of leprosy are fundamental to the understanding of the various forms of clinical manifestation of the disease. In some diseases, lymphocytes TH17 and one of its key cytokines, interleukin-17 has been shown to be essential in developing an effective immune response. In leprosy, involvement of lymphocyte TH17 and interleukin-17 remains understudied. OBJECTIVES: This study is the first investigation to examine the association between TH17 cells, interleukin-17 and interferon- γ in patients and households contacts of leprosy. METHODS: To document the participation of TH17 cells and interleukin-17 in the immunology of leprosy, to observe the behavior of interferon-γ in relation to interleukin-17 and to verify the differences found between individuals paucibacillary, multibacillary and household contacts, we analyzed samples peripheral blood to identify TH-17 cells, interleukin-17 and IFN-γ; establishing relationships between all the groups. RESULTS: There was a significant difference in the results found in the comparison between the paucibacillary and multibacillary groups of patients (P < 0.001), as well with the household contacts (P < 0.005). The polychemotherapeutic treatment modified the profile of immune response in multibacillary patients compared to what was observed before the start of treatment. CONCLUSION: The principal finding was that TH17 lymphocytes and interleukin-17 actively participating in the immune response of Hansen's disease as well these cells can stimulate the cellular immunity.

Signal-averaged electrocardiogram in chronic Chagas' heart disease.

UNLABELLED: The aim of the study was to register the prevalence of late potentials (LP) in patients with chronic Chagas' heart disease (CCD) and the relationship with sustained ventricular tachycardia (SVT). 192 patients (96 males), mean age 42.9 years, with CCD were studied through a Signal Averaged ECG using time domain analysis. According to presence or absence of bundle branch block (BBB) and SVT, four groups of patients were created: Group I (n = 72): without SVT (VT-) and without BBB (BBB-): Group II (n = 27): with SVT (VT+) and BBB-; Group III (n = 63): VT- and with BBB (BBB+); and Group IV (N = 30): VT+ and BBB+. The LP was admitted, with 40 Hz filter, in the groups without BBB using standard criteria of the method. In the group with BBB, the root-mean-square amplitude of the last 40 ms (RMS) < = 14 microV was considered as an indicator of LP. RESULTS: In groups I and II, LP was present in 21 (78%) of the patients with SVT and in 22 (31%) of the patients with SVT (p < 0.001), with Sensitivity (S) 78%; Specificity (SP) 70% and Accuracy (Ac) 72%. LP was present in 30 (48%) of the patients without and 20 (67%) of the patients with SVT, in groups III and IV. p = 0.066, with S = 66%; SP = 52%; and Ac = 57%. In the follow-up, there were 4 deaths unrelated to arrhythmic events, all of them did not have LP. Eight (29.6%) of the patients from group II and 4 (13%) from group IV presented recurrence of SVT and 91.6% of these patients had LP. CONCLUSIONS: LP occurred in 77.7% of patients with SVT and without BBB. In the groups with BBB, there was association of LP with SVT in 66.6% of the cases. The recurrence of SVT was patient in 21% of the cases from which 91.6% had LP.

[Value of computerized exercise stress test in the differential diagnosis of ischemic phenomena of obstructive versus nonobstructive origin]. / Valor do teste de esforço computadorizado no diagnóstico diferencial entre fenômenos isquêmicos de origem obstrutiva e não-obstrutiva.

PURPOSE: To describe groups of patients who have obstructive and non-obstructive coronary artery disease, through computadorized exercise stress test. METHODS: The test was done in 121 patients, all male, divided into 3 groups: GN group, 50 patients with normal electrocardiographic response to exercise; GLO group, 40 patients with obstructive coronary artery disease and GNO group, 31 patients with normal coronary arteries, showing one or more of the following entities: intramural coronary traject, coronary tortuosity, slow flow, mitral valve prolapse or left ventricular hypertrophy. GLO and GNO groups presented with abnormal response of the ST segment during exercise. The quantitative variables registered by computer were particularly analyzed as follows: STL (point Y depression), slope, index and ST segment integral. The magnitude of ST vector was visually measured and quantified. The statistic study was made through ANOVA and multiples comparison by the Scheffe's method, Fisher's test, quisquare and sensibility, specificity and accuracy calculation. RESULTS: There was a significant statistic difference among the 3 groups relative to slope and index (p < 0.05). The integral variable of ST segment did not allow us to differentiate the GLO and GNO groups. In the association study between the ST vector magnitude and abnormal T loop, there was an increase in sensibility of 15% in the exercise stress test. CONCLUSION: The ST segment slope below zero values, define patients having obstructive disease, and the opposite, non-obstructive disease. Values of ST segment index lower than -2 are linked to obstructive disease and higher than -2 linked to non-obstructive. Values of ST segment lower than -7 microV. s separate individuals with normal exercise stress test from those with ischemic type response. The magnitude of ST vector equal to or lower than 0.20mV define normal vectorcardiographic response to the exercise.

[Intermittent ventricular pre-excitation in the presence of total atrioventricular block]. / Pré-excitação ventricular intermitente na presença de bloqueio atrioventricular total.

An 8 year old child suffering from asthenia and complete atrioventricular block, probably congenital. During the tread-mill test there was a sudden electrocardiogram change to a ventricular preexcitation pattern. Besides this rare association, we emphasize the importance of the autonomic balance over the accessory pathway's electrophysiological properties. The appearance of a latent accessory pathway might be a substitutive mechanism to counterbalance the serious impairment of physiologic atrioventricular conduction.

Caracterizacao de uma instituicao nao-escolar e avaliacao de sua possivel interferencia no desenvolvimento da personalidade de parte de sua clientela (segunda parte). / Profiles of an extra-school institution and evaluation of its possible interference on the personality development of part of its clients (2d part)

Na etapa inicial desta pesquisa, os autores compararam a analise de uma instituicao nao-escolar para criancas de seis a catorze anos com os resultados de duas aplicacoes da prova de Rorschach com intervalo de um ano. Neste intervalo detectaram a evasao de quarenta das setenta criancas da amostragem inicial. Na presente etapa, propoem a pesquisa dos fatores que ponderam esse fenomeno. Apresentam os dados obtidos junto as familias da populacao evadida (que se mostram possiveis de explicar o fenomeno) bem como discutem aplicacao da prova de Rorschach junto as criancas desta segunda populacao