[Blood serum proteins in diabetes mellitus. Euglobulin level]. / Belki syvorotki krovi pri sakharnom diabete. Issledovanie euglobulinov.

Some physicochemical and immunochemical properties of blood serum euglobulins were studied in patients with diabetes mellitus with different degree of vascular lesions. Euglobulins were isolated from blood serum of normal subjects and diabetes mellitus patients by precipitation in low ionic strength dialyzing solution. As a result two euglobulin subfractions were obtained: soluble and insoluble in an isotonic saline. To study euglobulins, use was made of radioimmunoassay, gel filtration, and radial immunodiffusion. The data obtained indicate that patients with diabetes mellitus showed a significant increase in euglobulin content in both subfractions (P less than 0.001). Besides, the insoluble subfraction demonstrated a new protein component with a molecular mass about 265000 dalton, uncommon to healthy donors. There was a dramatic increase (P less than 0.0001) in the amount of euglobulin bound insulin, an insignificant part of which was combined with insulin antibodies and greater part with unidentified euglobulin components. The changes in the physicochemical and immunochemical properties of serum globulins in diabetes mellitus patients correlated with the gravity of vascular lesions as regards the majority of the parameters. It is concluded that these changes are possibly involved in the pathogenesis of diabetic angiopathies.

[Blood serum euglobulins in diabetes mellitus and diabetic microangiopathies]. / Syvorotochnye euglobuliny pri sakharnom diabete i diabeticheskikh microangiopatiiakh

A study of serum euglobulins carried out in 26 patients with diabetes mellitus and in 14 healthy persons permitted to detect definite differences between sick and healthy individuals. In the patients with diabetes mellitus (most distinctly in those with microangiopathies) the level of soluble and insoluble in the phosphate buffer euglobulins was greater than in the healthy persons. The microprecipitation reaction and immunoelectrophoresis with the monospecific immunoglobulins (IgA, IgM and IgG) more frequent and higher titers demonstrated IgA and IgG in diabetic patients. More pronounced heterogeneity (particularly distinct in the subfraction of euglobulins insoluble in the phosphate buffer) than in healthy individuals was revealed in the patients with diabetic microangiopathies by disc electrophoresis in polyacrylamide gel. The data obtained served as the basis for the search in the serum euglobulins of the patients with diabetes of compounds possibly possessing the antigenic properties, capable of producing antibodies and immune complexes, i.e. the immunological factors taking part in the vascular wall injury.