RESUMO
Systemic injury plays a central role in severe acute pancreatitis (SAP). Retrograde biliopancreatic duct infusion of sodium taurocholate (NaT) is commonly used to establish SAP animal models. To better characterize the systemic injury in this model, SAP was induced in Sprague-Dawley rats by NaT administration (3.5 or 5%), followed by sacrifice at 3, 6, 9, 12, 24, 48 and 72 h. Normal saline was used as a control in Sham-operated rats. The mortality rate, ascites volume, and serum and ascitic fluid amylase and lipase activities were assessed. Multiple organ dysfunction, including dysfunction of the pancreas, lung, ileum, liver, and kidney, was investigated using hematoxylin and eosin staining. The interleukin (IL)-1ß, IL-6, and tumor necrosis factor-α levels in the ascitic fluid, serum, and ileum tissues were evaluated using an enzyme-linked immunosorbent assay (ELISA). Tight junction proteins, zonula occludens-1 (ZO-1) and occludin, in ileum tissues were studied using immunofluorescence. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (CRE) and urea levels were measured using an automatic biochemical analyzer. The results of the present study indicated that both 3.5 and 5% NaT could induce a stable elevation of pancreatitis indices, with histopathological injury of the pancreas, lungs and ileum (5% NaT). The ascitic fluid levels of IL-6 and IL-1ß were increased in the 5% NaT group. ALT and AST levels increased temporarily and recovered in 72 h, without a significant increase in CRE and urea levels or apparent hepatic and renal pathological injury. In conclusion, rats with NaT-induced SAP have characteristics of necrotizing hemorrhagic pancreatitis with multiple organ injuries, including inflammatory lung injury, ischemic intestinal injury and slight liver and kidney injuries.
RESUMO
Objective To investigate the clinical effect of different doses of levothyroxine in treatment of subclinical hypothyroidism and its effect on blood lipid level.Methods 98 cases of subclinical hypothyroidism elderly patients accepted in our hospital from March 2013 to May 2016 were divided into group A and B with 49 patients in each.The two groups were treated with different doses of levothyroxine treatment for 6 months.Then the clinical efficacy,thyroid function,blood lipid levels and adverse reactions of two groups were compared.Results The cure rate and total effective rate of group A were 59.18％ and 87.76％ respectively.The cure rate and total effective rate of group B were 61.22％ and 91.84％ respectively.There was no significant differencebetween two groups.After treatment,thethyroid function and blood lipid levels of two groups were better than before treatment.After 4 weeks of treatment,the thyroid function and blood lipid level in group B were better than those in group A (P ＜0.05).There was no significant difference in thyroid function and blood lipid between the two groups after 12 weeks of treatment.The incidence of total adverse reactions in group A was 4.08％,which was significantly lower than that in group B (16.33％),the difference was statistically significant (P ＜ 0.05).Conclusion Different doses of levothyroxine in the treatment ofsubclinical hypothyroidism were similar,and the thyroid function and blood lipids levels were improved after treatment,but the adverse reaction rate of low dose group was less.
RESUMO
Objective To investigate the clinical effect of different doses of levothyroxine in treatment of subclinical hypothyroidism and its effect on blood lipid level.Methods 98 cases of subclinical hypothyroidism elderly patients accepted in our hospital from March 2013 to May 2016 were divided into group A and B with 49 patients in each.The two groups were treated with different doses of levothyroxine treatment for 6 months.Then the clinical efficacy,thyroid function,blood lipid levels and adverse reactions of two groups were compared.Results The cure rate and total effective rate of group A were 59.18％ and 87.76％ respectively.The cure rate and total effective rate of group B were 61.22％ and 91.84％ respectively.There was no significant differencebetween two groups.After treatment,thethyroid function and blood lipid levels of two groups were better than before treatment.After 4 weeks of treatment,the thyroid function and blood lipid level in group B were better than those in group A (P ＜0.05).There was no significant difference in thyroid function and blood lipid between the two groups after 12 weeks of treatment.The incidence of total adverse reactions in group A was 4.08％,which was significantly lower than that in group B (16.33％),the difference was statistically significant (P ＜ 0.05).Conclusion Different doses of levothyroxine in the treatment ofsubclinical hypothyroidism were similar,and the thyroid function and blood lipids levels were improved after treatment,but the adverse reaction rate of low dose group was less.
