RESUMO
Detecting and concentrating cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrode pattern can be dynamically changed by varied light patterns. By changing the projected light pattern, it is demonstrated to separate high-purity gastric cancer cell lines. Traditionally, the purity of cancer cell isolation by negative selection is 0.9% to 10%; by positive selection it is 50% to 62%. An ODEP technology is proposed to enhance the purity of cancer cell isolation to about 77%.
Assuntos
Separação Celular/métodos , Eletroforese/métodos , Técnicas Analíticas Microfluídicas/instrumentação , Imagem Óptica/métodos , Linhagem Celular Tumoral , Separação Celular/instrumentação , Eletroforese/instrumentação , Desenho de Equipamento , Humanos , Células MCF-7 , Neoplasias , Imagem Óptica/instrumentaçãoRESUMO
The detection and concentration of cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically-induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrodes pattern can be dynamically changed by varied light pattern. In this paper, a special lens is used to project the entire image to the ODEP chip to achieve 2.6 × 2 mm(2) manipulating area. By changing projected light pattern, it is demonstrated to separate 10, 20, and 40 µm PS (polystyrene) beads; HT-29, 20 µm PS beads. The MCF-7 cells concentrated experiments are also demo at 100 µm/sec velocity.
Assuntos
Neoplasias/diagnóstico , Células Neoplásicas Circulantes/patologia , Separação Celular/métodos , Detecção Precoce de Câncer , Eletroforese/métodos , Células HT29 , Humanos , Células MCF-7 , Técnicas Analíticas MicrofluídicasRESUMO
Metal-dielectric multiple-band high-reflection coatings are designed as induced filters and fabricated by reactive deposition. Ta(2)O(5) and SiO(2) are used as high- and low-refractive-index layers, and Cr and Al are used as bonding and reflective layers, respectively, for constructing the filters. The metal-dielectric coatings are deposited on a light-shaping flexible plastic substrate for use as a screen with high-contrast enhancement performance. This screen was suitable for miniprojectors with red, green, and blue LEDs as light sources. Mechanical properties such as stress, hardness, and adhesive strength of these multilayer films are investigated also.
